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Study Information
Study Attribute Value
CEBS Accession Number: 002-01244-0002-0000-5
Chemical Name: 2,5-Toluenediamine sulfate
CASRN: 6369-59-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 951026_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Positive 
No Activation  3  Weakly Positive* 
No Activation  4  Weakly Positive 
No Activation  5  Negative 

Trial #:1   Activation: No Activation    Date: 06/05/1985    Harvest Time: 14 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 0 0.000 1.0 0 0.000 0.0
Test Chemical: 2,5-Toluenediamine sulfate 
10         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
20         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
40         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
60         
 100 9 0.090 7.0 2 0.020 2.0 7 0.070 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 20 0.200 19.0 15 0.150 15.0 5 0.050 5.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 31 0.620 44.0 16 0.320 26.0 15 0.300 24.0 0 0.000 0.0
Trend: 3.103 1.990 2.704  
Probability: 0.001 0.023 0.003  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/17/1984    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: 2,5-Toluenediamine sulfate 
75         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
100         
 100 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0 0 0.000 0.0 
125         
 100 2 0.020 1.0 2 0.020 1.0 0 0.000 0.0 0 0.000 0.0 
150         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 38 0.380 27.0 21 0.210 17.0 17 0.170 14.0 0 0.000 0.0
Trend: -0.439 0.548 -1.166  
Probability: 0.670 0.292 0.878  


Trial #:2   Activation: No Activation    Date: 03/13/1985    Harvest Time: 12.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,5-Toluenediamine sulfate 
40         
 100 6 0.060 5.0 1 0.010 1.0 5 0.050 4.0 0 0.000 0.0 
60         
 100 16 0.160 10.0 8 0.080 5.0 8 0.080 8.0 0 0.000 0.0 
80         
 100 12 0.120 11.0 5 0.050 5.0 7 0.070 6.0 0 0.000 0.0 
100         
 100 16 0.160 14.0 2 0.020 2.0 14 0.140 13.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 19 0.190 16.0 8 0.080 7.0 11 0.110 11.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 12 0.240 22.0 8 0.160 16.0 4 0.080 8.0 0 0.000 0.0
Trend: 3.724 1.359 3.626  
Probability: 0.000 0.087 0.000  


Trial #:3   Activation: No Activation    Date: 10/16/1985    Harvest Time: 17.5 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: 2,5-Toluenediamine sulfate 
25         
 200 4 0.020 2.0 1 0.005 0.5 3 0.015 1.5 0 0.000 0.0 
50         
 200 91 0.455 25.0 21 0.105 10.0 50 0.250 17.5 20 0.100 2.0
Positive Control: Mitomycin-C 
 0.0625    
 200 114 0.570 35.5 56 0.280 20.5 58 0.290 23.5 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 38 0.760 50.0 18 0.360 32.0 20 0.400 34.0 0 0.000 0.0
Trend: 9.156 6.126 7.322  
Probability: 0.000 0.000 0.000  


Trial #:4   Activation: No Activation    Date: 10/16/1985    Harvest Time: 12.5 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: 2,5-Toluenediamine sulfate 
25         
 200 6 0.030 3.0 1 0.005 0.5 5 0.025 2.5 0 0.000 0.0 
50         
 200 16 0.080 7.0 2 0.010 1.0 14 0.070 6.5 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 29 0.145 13.5 9 0.045 4.0 20 0.100 10.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 16 0.320 28.0 5 0.100 10.0 11 0.220 22.0 0 0.000 0.0
Trend: 3.072 1.228 3.056  
Probability: 0.001 0.110 0.001  


Trial #:5   Activation: No Activation    Date: 10/17/1984    Harvest Time: 13.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 2,5-Toluenediamine sulfate 
1         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
5         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
10         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
25         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 16 0.160 16.0 10 0.100 10.0 6 0.060 6.0 0 0.000 0.0
Mitomycin-C 
1         
 50 31 0.620 46.0 24 0.480 44.0 7 0.140 14.0 0 0.000 0.0
Trend: -0.555 -0.781 -0.126  
Probability: 0.711 0.783 0.550