Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01244-0002-0000-5 |
Chemical Name: | 2,5-Toluenediamine sulfate |
CASRN: | 6369-59-1 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 951026_CA |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Weakly Positive |
Induced Rat Liver S9 | 1_S9 | Negative |
No Activation | 2 | Positive |
No Activation | 3 | Weakly Positive* |
No Activation | 4 | Weakly Positive |
No Activation | 5 | Negative |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 1.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,5-Toluenediamine sulfate | 10 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
20 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
40 | 100 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
60 | 100 | 9 | 0.090 | 7.0 | 2 | 0.020 | 2.0 | 7 | 0.070 | 6.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.125 | 100 | 20 | 0.200 | 19.0 | 15 | 0.150 | 15.0 | 5 | 0.050 | 5.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 31 | 0.620 | 44.0 | 16 | 0.320 | 26.0 | 15 | 0.300 | 24.0 | 0 | 0.000 | 0.0 | |
Trend: | 3.103 | 1.990 | 2.704 | ||||||||||||
Probability: | 0.001 | 0.023 | 0.003 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,5-Toluenediamine sulfate | 75 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
100 | 100 | 3 | 0.030 | 3.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
125 | 100 | 2 | 0.020 | 1.0 | 2 | 0.020 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
150 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 15 | 100 | 38 | 0.380 | 27.0 | 21 | 0.210 | 17.0 | 17 | 0.170 | 14.0 | 0 | 0.000 | 0.0 |
Trend: | -0.439 | 0.548 | -1.166 | ||||||||||||
Probability: | 0.670 | 0.292 | 0.878 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,5-Toluenediamine sulfate | 40 | 100 | 6 | 0.060 | 5.0 | 1 | 0.010 | 1.0 | 5 | 0.050 | 4.0 | 0 | 0.000 | 0.0 |
60 | 100 | 16 | 0.160 | 10.0 | 8 | 0.080 | 5.0 | 8 | 0.080 | 8.0 | 0 | 0.000 | 0.0 | ||
80 | 100 | 12 | 0.120 | 11.0 | 5 | 0.050 | 5.0 | 7 | 0.070 | 6.0 | 0 | 0.000 | 0.0 | ||
100 | 100 | 16 | 0.160 | 14.0 | 2 | 0.020 | 2.0 | 14 | 0.140 | 13.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.125 | 100 | 19 | 0.190 | 16.0 | 8 | 0.080 | 7.0 | 11 | 0.110 | 11.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 12 | 0.240 | 22.0 | 8 | 0.160 | 16.0 | 4 | 0.080 | 8.0 | 0 | 0.000 | 0.0 | |
Trend: | 3.724 | 1.359 | 3.626 | ||||||||||||
Probability: | 0.000 | 0.087 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,5-Toluenediamine sulfate | 25 | 200 | 4 | 0.020 | 2.0 | 1 | 0.005 | 0.5 | 3 | 0.015 | 1.5 | 0 | 0.000 | 0.0 |
50 | 200 | 91 | 0.455 | 25.0 | 21 | 0.105 | 10.0 | 50 | 0.250 | 17.5 | 20 | 0.100 | 2.0 | ||
Positive Control: | Mitomycin-C | 0.0625 | 200 | 114 | 0.570 | 35.5 | 56 | 0.280 | 20.5 | 58 | 0.290 | 23.5 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.125 | 50 | 38 | 0.760 | 50.0 | 18 | 0.360 | 32.0 | 20 | 0.400 | 34.0 | 0 | 0.000 | 0.0 | |
Trend: | 9.156 | 6.126 | 7.322 | ||||||||||||
Probability: | 0.000 | 0.000 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,5-Toluenediamine sulfate | 25 | 200 | 6 | 0.030 | 3.0 | 1 | 0.005 | 0.5 | 5 | 0.025 | 2.5 | 0 | 0.000 | 0.0 |
50 | 200 | 16 | 0.080 | 7.0 | 2 | 0.010 | 1.0 | 14 | 0.070 | 6.5 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.0625 | 200 | 29 | 0.145 | 13.5 | 9 | 0.045 | 4.0 | 20 | 0.100 | 10.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.125 | 50 | 16 | 0.320 | 28.0 | 5 | 0.100 | 10.0 | 11 | 0.220 | 22.0 | 0 | 0.000 | 0.0 | |
Trend: | 3.072 | 1.228 | 3.056 | ||||||||||||
Probability: | 0.001 | 0.110 | 0.001 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,5-Toluenediamine sulfate | 1 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
5 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
10 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
25 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.125 | 100 | 16 | 0.160 | 16.0 | 10 | 0.100 | 10.0 | 6 | 0.060 | 6.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 1 | 50 | 31 | 0.620 | 46.0 | 24 | 0.480 | 44.0 | 7 | 0.140 | 14.0 | 0 | 0.000 | 0.0 | |
Trend: | -0.555 | -0.781 | -0.126 | ||||||||||||
Probability: | 0.711 | 0.783 | 0.550 |