Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01244-0008-0000-1
Chemical Name:	2,5-Toluenediamine sulfate
CASRN:	6369-59-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	821795
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	101	89	106	35	40
			102	88	139	45
			108	129	141	43
			106	94	115	36
Test Chemical	2,5-Toluenediamine sulfate	0.625	87	58	156	60	56
			91	79	143	52	56
		1.25	107	90	178	56	53
			105	88	157	50	53
		2.5	88	83	204	77	66*
			87	73	143	55	66*
		5	85	66	335	132	99*
			80	68	160	67	99*
		10	62	10	168	91	136*
			65	12	350	180	136*
		20

Positive Control	Methyl Methane Sulfonate	15	45	23	238	176	157*
Positive Control			63	29	258	137	157*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	59	103	211	119	93
			83	93	206	83
			90	117	200	74
			74	87	209	94
Test Chemical	2,5-Toluenediamine sulfate	3	77	105	131	57	60
			85	90	159	62	60
		4	73	94	196	89	78
			92	90	185	67	78
		5	95	89	220	77	75
			83	95	179	72	75
		6	77	79	195	84	81
			68	86	158	78	81
		7	67	82	163	82	79
			71	86	160	75	79
Positive Control	Methyl Methane Sulfonate	15	30	22	209	234	250*
Positive Control			34#	23	271.5	266	250*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	79	88	64	27	24
			87	108	66	25
			96	95	66	23
			106	109	62	19
Test Chemical	2,5-Toluenediamine sulfate	2	99	67	97	33	31
			84	69	73	29	31
		3	85	115	79	31	30
			78	80	66	28	30
		4	75	28	152	68	59*
			72	27	108	50	59*
		5	74	9	167	76	75*
			56	5	123	74	75*
		6	58	4	147	85	83*
			71	5	173	81	83*
		7
		8

Positive Control	Methyl Methane Sulfonate	15	24	15	212	292	239*
Positive Control			42	27	236	186	239*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination