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Study Information
Study Attribute Value
CEBS Accession Number: 002-01244-0008-0000-1
Chemical Name: 2,5-Toluenediamine sulfate
CASRN: 6369-59-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 821795
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 101 89 106 35 40
    102 88 139 45
    108 129 141 43
    106 94 115 36
Test Chemical 2,5-Toluenediamine sulfate 
 0.625     
 87 58 156 60 56
    91 79 143 52
  
1.25      
 107 90 178 56 53
    105 88 157 50
  
2.5       
 88 83 204 77 66*
    87 73 143 55
  
5         
 85 66 335 132 99*
    80 68 160 67
  
10         
 62 10 168 91 136*
    65 12 350 180
  
20         
   
Positive Control Methyl Methane Sulfonate 
15         
 45 23 238 176 157*
    63 29 258 137

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 59 103 211 119 93
    83 93 206 83
    90 117 200 74
    74 87 209 94
Test Chemical 2,5-Toluenediamine sulfate 
3         
 77 105 131 57 60
    85 90 159 62
  
4         
 73 94 196 89 78
    92 90 185 67
  
5         
 95 89 220 77 75
    83 95 179 72
  
6         
 77 79 195 84 81
    68 86 158 78
  
7         
 67 82 163 82 79
    71 86 160 75
Positive Control Methyl Methane Sulfonate 
15         
 30 22 209 234 250*
    34# 23 271.5 266

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 79 88 64 27 24
    87 108 66 25
    96 95 66 23
    106 109 62 19
Test Chemical 2,5-Toluenediamine sulfate 
2         
 99 67 97 33 31
    84 69 73 29
  
3         
 85 115 79 31 30
    78 80 66 28
  
4         
 75 28 152 68 59*
    72 27 108 50
  
5         
 74 9 167 76 75*
    56 5 123 74
  
6         
 58 4 147 85 83*
    71 5 173 81
  
7         
  
8         
   
Positive Control Methyl Methane Sulfonate 
15         
 24 15 212 292 239*
    42 27 236 186

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination