COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01246-0001-0000-6
Chemical Name: 2,6-Dichloro-p-phenylenediamine
CASRN: 609-20-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 402554_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 03/28/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1046 402 0.384 8.04 26.5 -2.657
Dimethyl Sulfoxide 
0         
 50 1041 411 0.395 8.22 26.5 0.000
Test Chemical 2,6-Dichloro-p-phenylenediamine 
1.6       
 50 1027 392 0.382 7.84 26.5 -3.323 
5         
 50 1027 393 0.383 7.86 26.5 -3.076 
16         
 50 1039 530 0.510 10.60 26.5 29.202 
50         
 50 1024 515 0.503 10.30 26.5 27.384 
160         
 0 0 0 0.000 0.00 26.5 N/A 
500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1047 1448 1.383 28.96 26.5 250.292
Trend: 5.318        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 04/07/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1034 374 0.362 7.48 27.0 -7.042
Dimethyl Sulfoxide 
0         
 50 1046 407 0.389 8.14 27.0 0.000
Test Chemical 2,6-Dichloro-p-phenylenediamine 
100         
 50 1040 677 0.651 13.54 32.0 67.299 
200         
 50 1051 839 0.798 16.78 42.0 105.162 
300         
 50 1047 1235 1.180 24.70 42.0 203.150 
400         
 50 1039 1284 1.236 25.68 42.0 217.605 
500         
 50 1041 1331 1.279 26.62 42.0 228.598 
750         
 0 0 0 0.000 0.00 42.0 N/A 
1000         
 0 0 0 0.000 0.00 42.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1048 1481 1.413 29.62 27.0 263.188
Trend: 26.890        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 03/28/1983    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1026 399 0.389 7.98 26.5 -7.661
Dimethyl Sulfoxide 
0         
 50 1040 438 0.421 8.76 26.5 0.000
Test Chemical 2,6-Dichloro-p-phenylenediamine 
50         
 50 1042 449 0.431 8.98 26.5 2.315 
160         
 50 1034 453 0.438 9.06 26.5 4.025 
500         
 50 1031 476 0.462 9.52 26.5 9.624 
1600         
 3 63 39 0.619 13.00 26.5 46.988
Positive Control Cyclophosphamide 
1.5       
 50 1040 1369 1.316 27.38 26.5 212.557
Trend: 1.913        
Probability: 0.028        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 04/07/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1040 416 0.400 8.32 26.0 -2.600
Dimethyl Sulfoxide 
0         
 50 1030 423 0.411 8.46 26.0 0.000
Test Chemical 2,6-Dichloro-p-phenylenediamine 
500         
 50 1032 474 0.459 9.48 26.0 11.840 
750         
 50 1045 553 0.529 11.06 26.0 28.856 
1000         
 50 1044 563 0.539 11.26 32.0 31.312 
2000         
 0 0 0 0.000 0.00 41.5 N/A 
3000         
 0 0 0 0.000 0.00 41.5 N/A 
4000         
 0 0 0 0.000 0.00 41.5 N/A
Positive Control Cyclophosphamide 
1.5       
 50 1040 932 0.896 18.64 26.0 118.212
Trend: 4.786        
Probability: 0.000