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Study Information
Study Attribute Value
CEBS Accession Number: 002-01246-0002-0000-7
Chemical Name: 2,6-Dichloro-p-phenylenediamine
CASRN: 609-20-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 402554_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Weakly Positive* 
Induced Rat Liver S9  2_S9  Weakly Positive* 

Trial #:1   Activation: No Activation    Date: 04/26/1983    Harvest Time: 15 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: 2,6-Dichloro-p-phenylenediamine 
100         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
100         
 100 7 0.070 6.0 3 0.030 3.0 4 0.040 4.0 0 0.000 0.0 
250         
 100 15 0.150 14.0 8 0.080 8.0 6 0.060 6.0 1 0.010 1.0 
250         
 100 5 0.050 5.0 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 
500         
 100 11 0.110 9.0 7 0.070 6.0 4 0.040 3.0 0 0.000 0.0 
750         
 100 10 0.100 8.0 0 0.000 0.0 10 0.100 8.0 0 0.000 0.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.5       
 100 129 1.290 68.0 69 0.690 52.0 60 0.600 44.0 0 0.000 0.0
Trend: 2.112 0.791 1.883  
Probability: 0.017 0.214 0.030  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/26/1983    Harvest Time: 15.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,6-Dichloro-p-phenylenediamine 
1000         
 100 31 0.310 24.0 23 0.230 18.0 8 0.080 8.0 0 0.000 0.0 
1000         
 100 40 0.400 25.0 32 0.320 22.0 6 0.060 5.0 2 0.020 2.0 
2000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
3000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
4000         
 100 41 0.410 29.0 18 0.180 14.0 23 0.230 20.0 0 0.000 0.0 
5000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 55 0.550 38.0 27 0.270 24.0 28 0.280 28.0 0 0.000 0.0
Trend: 3.589 0.979 5.217  
Probability: 0.000 0.164 0.000  


Trial #:2   Activation: No Activation    Date: 03/30/1982    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 2,6-Dichloro-p-phenylenediamine 
50         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
160         
 100 5 0.050 5.0 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 
500         
 100 11 0.110 10.0 7 0.070 7.0 3 0.030 3.0 1 0.010 1.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1600         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 37 0.370 26.0 15 0.150 14.0 22 0.220 18.0 0 0.000 0.0
Trend: 3.797 3.545 1.568  
Probability: 0.000 0.000 0.058  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 03/30/1983    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 4 0.040 3.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: 2,6-Dichloro-p-phenylenediamine 
160         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 12 0.120 9.0 8 0.080 6.0 4 0.040 3.0 0 0.000 0.0 
1600         
 100 24 0.240 19.0 11 0.110 10.0 11 0.110 10.0 2 0.020 2.0 
5000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 79 0.790 34.0 30 0.300 22.0 19 0.190 15.0 30 0.300 3.0
Trend: 4.621 3.050 3.185  
Probability: 0.000 0.001 0.001