Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01246-0002-0000-7 |
Chemical Name: | 2,6-Dichloro-p-phenylenediamine |
CASRN: | 609-20-1 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 402554_CA |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Weakly Positive |
Induced Rat Liver S9 | 1_S9 | Positive |
No Activation | 2 | Weakly Positive* |
Induced Rat Liver S9 | 2_S9 | Weakly Positive* |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 4 | 0.040 | 4.0 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | 2,6-Dichloro-p-phenylenediamine | 100 | 100 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
100 | 100 | 7 | 0.070 | 6.0 | 3 | 0.030 | 3.0 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | ||
250 | 100 | 15 | 0.150 | 14.0 | 8 | 0.080 | 8.0 | 6 | 0.060 | 6.0 | 1 | 0.010 | 1.0 | ||
250 | 100 | 5 | 0.050 | 5.0 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
500 | 100 | 11 | 0.110 | 9.0 | 7 | 0.070 | 6.0 | 4 | 0.040 | 3.0 | 0 | 0.000 | 0.0 | ||
750 | 100 | 10 | 0.100 | 8.0 | 0 | 0.000 | 0.0 | 10 | 0.100 | 8.0 | 0 | 0.000 | 0.0 | ||
1000 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.5 | 100 | 129 | 1.290 | 68.0 | 69 | 0.690 | 52.0 | 60 | 0.600 | 44.0 | 0 | 0.000 | 0.0 |
Trend: | 2.112 | 0.791 | 1.883 | ||||||||||||
Probability: | 0.017 | 0.214 | 0.030 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | 2,6-Dichloro-p-phenylenediamine | 1000 | 100 | 31 | 0.310 | 24.0 | 23 | 0.230 | 18.0 | 8 | 0.080 | 8.0 | 0 | 0.000 | 0.0 |
1000 | 100 | 40 | 0.400 | 25.0 | 32 | 0.320 | 22.0 | 6 | 0.060 | 5.0 | 2 | 0.020 | 2.0 | ||
2000 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
3000 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
4000 | 100 | 41 | 0.410 | 29.0 | 18 | 0.180 | 14.0 | 23 | 0.230 | 20.0 | 0 | 0.000 | 0.0 | ||
5000 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 50 | 100 | 55 | 0.550 | 38.0 | 27 | 0.270 | 24.0 | 28 | 0.280 | 28.0 | 0 | 0.000 | 0.0 |
Trend: | 3.589 | 0.979 | 5.217 | ||||||||||||
Probability: | 0.000 | 0.164 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | 2,6-Dichloro-p-phenylenediamine | 50 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
160 | 100 | 5 | 0.050 | 5.0 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
500 | 100 | 11 | 0.110 | 10.0 | 7 | 0.070 | 7.0 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 | ||
1000 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
1600 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
2000 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.25 | 100 | 37 | 0.370 | 26.0 | 15 | 0.150 | 14.0 | 22 | 0.220 | 18.0 | 0 | 0.000 | 0.0 |
Trend: | 3.797 | 3.545 | 1.568 | ||||||||||||
Probability: | 0.000 | 0.000 | 0.058 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Negative (Not Specified) | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Dimethyl Sulfoxide | 0 | 100 | 4 | 0.040 | 3.0 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | |
Test Chemical: | 2,6-Dichloro-p-phenylenediamine | 160 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
500 | 100 | 12 | 0.120 | 9.0 | 8 | 0.080 | 6.0 | 4 | 0.040 | 3.0 | 0 | 0.000 | 0.0 | ||
1600 | 100 | 24 | 0.240 | 19.0 | 11 | 0.110 | 10.0 | 11 | 0.110 | 10.0 | 2 | 0.020 | 2.0 | ||
5000 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 50 | 100 | 79 | 0.790 | 34.0 | 30 | 0.300 | 22.0 | 19 | 0.190 | 15.0 | 30 | 0.300 | 3.0 |
Trend: | 4.621 | 3.050 | 3.185 | ||||||||||||
Probability: | 0.000 | 0.001 | 0.001 |