Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01246-0011-0000-7
Chemical Name:	2,6-Dichloro-p-phenylenediamine
CASRN:	609-20-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	789062
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	96	18	9	19
			78	97	48	20
			77	114	35	15
			73	93	68	31
Test Chemical	2,6-Dichloro-p-phenylenediamine	12.5	75	110	46	21	23
			71	98	54	26	23
		25	81	106	86	36	34*
			80	102	77	32	34*
		50	44	18	234	177	169*
			52	15	253	162	169*
		100	37	7	213	192	180*
			39	8	194	167	180*
		200	17	2	105	212	207*
			19	2	113	202	207*
		400

Positive Control	Methyl Methane Sulfonate	15	55	42	141	85	98*
			48	48	160	111	98*
	Methyl Methane Sulfonate	250	66	89	324	164	144*
			84	85	311	123	144*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	91	59	31	35
			75	114	84	37
			71	102	81	38
			64	93	63	33
Test Chemical	2,6-Dichloro-p-phenylenediamine	25	78	107	72	31	32
			66	105	65	33	32
		50	64	72	116	61	71*
			55	73	134	81	71*
		100	38	10	211	186	194*
			45	12	272	203	194*
		150	37	7	201	182	167*
			40	8	182	152	167*
		200	30	5	132	147	148*
			27	5	121	149	148*
		250

		300

Positive Control	Methyl Methane Sulfonate	15	38	23	152	133	134*
			35	26	141	135	134*
	Methyl Methane Sulfonate	250	60	61	333	186	175*
			64	77	313	164	175*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination