Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01246-0011-0000-7 |
Chemical Name: | 2,6-Dichloro-p-phenylenediamine |
CASRN: | 609-20-1 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 789062 |
Study Result: | Positive |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 69 | 96 | 18 | 9 | 19 |
78 | 97 | 48 | 20 | ||||
77 | 114 | 35 | 15 | ||||
73 | 93 | 68 | 31 | ||||
Test Chemical | 2,6-Dichloro-p-phenylenediamine | 12.5 | 75 | 110 | 46 | 21 | 23 |
71 | 98 | 54 | 26 | ||||
25 | 81 | 106 | 86 | 36 | 34* | ||
80 | 102 | 77 | 32 | ||||
50 | 44 | 18 | 234 | 177 | 169* | ||
52 | 15 | 253 | 162 | ||||
100 | 37 | 7 | 213 | 192 | 180* | ||
39 | 8 | 194 | 167 | ||||
200 | 17 | 2 | 105 | 212 | 207* | ||
19 | 2 | 113 | 202 | ||||
400 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 55 | 42 | 141 | 85 | 98* |
48 | 48 | 160 | 111 | ||||
Methyl Methane Sulfonate | 250 | 66 | 89 | 324 | 164 | 144* | |
84 | 85 | 311 | 123 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 64 | 91 | 59 | 31 | 35 |
75 | 114 | 84 | 37 | ||||
71 | 102 | 81 | 38 | ||||
64 | 93 | 63 | 33 | ||||
Test Chemical | 2,6-Dichloro-p-phenylenediamine | 25 | 78 | 107 | 72 | 31 | 32 |
66 | 105 | 65 | 33 | ||||
50 | 64 | 72 | 116 | 61 | 71* | ||
55 | 73 | 134 | 81 | ||||
100 | 38 | 10 | 211 | 186 | 194* | ||
45 | 12 | 272 | 203 | ||||
150 | 37 | 7 | 201 | 182 | 167* | ||
40 | 8 | 182 | 152 | ||||
200 | 30 | 5 | 132 | 147 | 148* | ||
27 | 5 | 121 | 149 | ||||
250 | |||||||
300 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 38 | 23 | 152 | 133 | 134* |
35 | 26 | 141 | 135 | ||||
Methyl Methane Sulfonate | 250 | 60 | 61 | 333 | 186 | 175* | |
64 | 77 | 313 | 164 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination