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Study Information
Study Attribute Value
CEBS Accession Number: 002-01250-0001-0000-1
Chemical Name: 2,6-Diisocyanatotoluene
CASRN: 91-08-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 618809_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 04/24/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 384 0.366 7.68 26.5 0.000
Test Chemical 2,6-Diisocyanatotoluene 
5         
 50 1053 434 0.412 8.68 26.5 12.484 
16         
 50 1047 451 0.431 9.02 26.5 17.560 
50         
 50 1051 447 0.425 8.94 26.5 16.074 
160         
 50 1047 572 0.546 11.44 26.5 49.101 
500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1046 582 0.556 11.64 26.5 51.852
Mitomycin-C 
 0.005     
 10 207 260 1.256 26.00 26.5 242.794
Trend: 5.783        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 06/04/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 426 0.406 8.52 26.0 0.000
Test Chemical 2,6-Diisocyanatotoluene 
100         
 50 1050 628 0.598 12.56 26.0 47.418 
160         
 50 1049 627 0.598 12.54 26.0 47.323 
200         
 50 1047 582 0.556 11.64 26.0 37.011 
300         
 50 1049 697 0.664 13.94 26.0 63.771
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 550 0.524 11.00 26.0 29.108
Mitomycin-C 
 0.005     
 10 209 242 1.158 24.20 26.0 185.397
Trend: 6.666        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 07/02/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 472 0.451 9.44 26.0 0.000
Test Chemical 2,6-Diisocyanatotoluene 
50         
 50 1047 589 0.563 11.78 26.0 24.788 
160         
 50 1046 700 0.669 14.00 26.0 48.447 
200         
 50 1048 765 0.730 15.30 26.0 61.922 
300         
 50 1046 618 0.591 12.36 31.0 31.057
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 727 0.692 14.54 26.0 53.585
Mitomycin-C 
 0.005     
 10 210 272 1.295 27.20 26.0 187.312
Trend: 6.875        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/24/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 411 0.391 8.22 26.5 0.000
Test Chemical 2,6-Diisocyanatotoluene 
16         
 50 1048 446 0.426 8.92 26.5 8.723 
50         
 50 1047 403 0.385 8.06 26.5 -1.666 
160         
 50 1043 383 0.367 7.66 26.5 -6.187 
1600         
 50 1044 432 0.414 8.64 26.5 5.714 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1051 808 0.769 16.16 26.5 96.407
Cyclophosphamide 
 0.6       
 10 210 255 1.214 25.50 26.5 210.219
Trend: 0.063        
Probability: 0.475