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Study Information
Study Attribute Value
CEBS Accession Number: 002-01250-0002-0000-2
Chemical Name: 2,6-Diisocyanatotoluene
CASRN: 91-08-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 618809_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Weakly Positive* 
No Activation  3  Negative 

Trial #:1   Activation: No Activation    Date: 09/04/1985    Harvest Time: 18 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,6-Diisocyanatotoluene 
400         
 200 1 0.005 1.0 1 0.005 1.0 0 0.000 0.0 0 0.000 0.0 
600         
 200 5 0.025 3.0 2 0.010 1.0 3 0.015 2.0 0 0.000 0.0 
800         
 200 7 0.035 4.0 1 0.005 1.0 6 0.030 3.0 0 0.000 0.0 
1000         
 158 34 0.215 20.0 11 0.070 6.0 23 0.146 14.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 52 0.260 21.0 28 0.140 13.0 24 0.120 12.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 39 0.780 50.0 21 0.420 36.0 18 0.360 32.0 0 0.000 0.0
Trend: 7.412 3.495 6.499  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 06/26/1985    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 1.0 0 0.000 0.0 1 0.005 1.0 0 0.000 0.0
Test Chemical: 2,6-Diisocyanatotoluene 
160         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
300         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 200 3 0.015 2.0 2 0.010 1.0 1 0.005 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 123 0.615 41.0 43 0.215 18.0 80 0.400 31.0 0 0.000 0.0
Cyclophosphamide 
10         
 50 29 0.580 52.0 10 0.200 20.0 19 0.380 34.0 0 0.000 0.0
Trend: 0.564 1.847 -0.419  
Probability: 0.286 0.032 0.662  


Trial #:2   Activation: No Activation    Date: 08/07/1985    Harvest Time: 17.5 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 2.0 1 0.005 1.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: 2,6-Diisocyanatotoluene 
600         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0 
800         
 200 5 0.025 2.0 1 0.005 1.0 4 0.020 2.0 0 0.000 0.0 
1000         
 200 23 0.115 11.0 11 0.055 5.0 12 0.060 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 76 0.380 28.0 43 0.215 19.0 33 0.165 16.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 35 0.700 44.0 13 0.260 18.0 22 0.440 38.0 0 0.000 0.0
Trend: 4.087 3.048 2.825  
Probability: 0.000 0.001 0.002  


Trial #:3   Activation: No Activation    Date: 06/26/1985    Harvest Time: 18 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: 2,6-Diisocyanatotoluene 
300         
 200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 
500         
 200 1 0.005 1.0 0 0.000 0.0 1 0.005 1.0 0 0.000 0.0 
1000         
 200 2 0.010 1.0 1 0.005 1.0 1 0.005 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 200 161 0.805 47.0 77 0.385 30.0 74 0.370 28.0 10 0.050 1.0
Mitomycin-C 
 0.25      
 50 84 1.680 70.0 44 0.880 54.0 40 0.800 48.0 0 0.000 0.0
Trend: -0.267 0.978 0.000  
Probability: 0.605 0.164 0.500