Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01250-0004-0000-4
Chemical Name:	2,6-Diisocyanatotoluene
CASRN:	91-08-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	429581
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	115	61	28	29
			65	81	42	22
			67	117	48	24
			53	86	69	43
Test Chemical	2,6-Diisocyanatotoluene	15.5625	76	96	74	33	27
			66	79	43	22	27
		31.125	56	67	52	31	35
			61	67	71	39	35
		62.5	69	61	86	41	50*
			71	50	127	59	50*
		125	55	24	127	77	77*
			53	19	123	78	77*
		250	34	5	227	224	177*
			61	10	238	130	177*
		500

Positive Control	Methyl Methane Sulfonate	15	27	18	120	150	146*
Positive Control			20	16	85	142	146*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	104	83	38	32
			63	103	61	33
			77	86	65	28
			75	107	63	28
Test Chemical	2,6-Diisocyanatotoluene	25	80	66	118	49	46
			77	57	97	42	46
		50	69	42	131	63	60*
			66	47	113	57	60*
		100	60	12	237	131	112*
			61	9	170	93	112*
		150

Positive Control	Methyl Methane Sulfonate	15	32	18	154	162	166*
Positive Control			32	23	164	171	166*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	99	74	33	38
			69	84	92	44
			71	113	71	33
			74	104	88	40
Test Chemical	2,6-Diisocyanatotoluene	25	76	74	119	53	42
			73	92	70	32	42
		50	73	44	138	63	54
			68	50	90	44	54
		100	75	52	142	63	75*
			66	37	171	87	75*
		150	56	28	154	91	90*
			59	27	157	89	90*
		200	57	11	142	84	96*
			51	15	163	107	96*
		250

Positive Control	3-Methylcholanthrene	2.5	54	30	388	240	284*
Positive Control			35	20	344	328	284*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	101	111	50	56
			49r	85	124	84
			57	96	91	53
			69	103	137	66
Test Chemical	2,6-Diisocyanatotoluene	10	57	99	106	62	66
			40	50	83	69	66
		25	33	43	116	118	95*
			53	66	114	72	95*
		50	38	30	140	123	132*
			40	34	169	141	132*
		100	29	8	222	251	229*
			34	9	213	207	229*
		150	34	6	258	250	227*
			36	10	219	203	227*
		200

Positive Control	3-Methylcholanthrene	2.5	39	29	674	571	521*
Positive Control			41	30	583	470	521*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination