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Study Information
Study Attribute Value
CEBS Accession Number: 002-01254-0001-0000-5
Chemical Name: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride)
CASRN: 15481-70-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 717361_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 04/23/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1046 426 0.407 8.52 26.5 0.000
Test Chemical 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
5         
 50 1049 416 0.397 8.32 26.5 -2.627 
16         
 50 1049 463 0.441 9.26 26.5 8.375 
50         
 50 1048 552 0.527 11.04 26.5 29.330 
160         
 50 1048 721 0.688 14.42 26.5 68.926 
500         
 0 0 0 0.000 0.00 26.5 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1050 577 0.550 11.54 26.5 34.930
Mitomycin-C 
 0.005     
 10 210 232 1.105 23.20 26.5 171.263
Trend: 10.095        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 06/04/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1049 429 0.409 8.58 26.0 0.000
Test Chemical 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
5         
 50 1050 453 0.431 9.06 26.0 5.494 
16         
 50 1047 479 0.457 9.58 26.0 11.868 
50         
 50 1044 604 0.579 12.08 26.0 41.467 
160         
 50 1049 692 0.660 13.84 26.0 61.305
Positive Control Mitomycin-C 
 0.001     
 50 1050 550 0.524 11.00 26.0 28.083
Mitomycin-C 
 0.005     
 10 209 242 1.158 24.20 26.0 183.131
Trend: 9.320        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/23/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1044 410 0.393 8.20 26.0 0.000
Test Chemical 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
16         
 50 1050 477 0.454 9.54 26.0 15.677 
50         
 50 1049 482 0.459 9.64 26.0 17.001 
160         
 50 1049 443 0.422 8.86 26.0 7.534 
500         
 50 1050 600 0.571 12.00 26.0 45.505
Positive Control Cyclophosphamide 
 0.3       
 50 1044 898 0.860 17.96 26.0 119.024
Cyclophosphamide 
 0.6       
 10 210 275 1.310 27.50 26.0 233.449
Trend: 4.904        
Probability: 0.000