COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01254-0002-0000-6
Chemical Name: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride)
CASRN: 15481-70-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 717361_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Weakly Positive* 
Induced Rat Liver S9  2_S9  Negative 
No Activation  3  Weakly Positive 
No Activation  4  Positive 

Trial #:1   Activation: No Activation    Date: 07/03/1985    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0
Test Chemical: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
500         
 200 8 0.040 3.5 2 0.010 1.0 6 0.030 2.5 0 0.000 0.0 
1000         
 200 9 0.045 4.0 1 0.005 0.5 8 0.040 3.5 0 0.000 0.0 
1600         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0 
3000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 37 0.185 14.5 14 0.070 6.5 23 0.115 9.5 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 8 0.160 14.0 1 0.020 2.0 6 0.120 12.0 1 0.020 1.0
Trend: 0.180 -0.217 0.277  
Probability: 0.429 0.586 0.391  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 07/03/1985    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
500         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0 
1000         
 200 8 0.040 2.5 0 0.000 0.0 8 0.040 2.5 0 0.000 0.0 
1600         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
3000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 50 0.250 20.5 13 0.065 6.5 37 0.185 16.5 0 0.000 0.0
Cyclophosphamide 
10         
 50 19 0.380 36.0 5 0.100 10.0 14 0.280 28.0 0 0.000 0.0
Trend: 0.785 -0.470 0.973  
Probability: 0.216 0.681 0.165  


Trial #:2   Activation: No Activation    Date: 10/16/1985    Harvest Time: 17.5 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Medium 
0         
 200 5 0.025 2.5 2 0.010 1.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
100         
 200 6 0.030 2.5 0 0.000 0.0 6 0.030 2.5 0 0.000 0.0 
250         
 200 8 0.040 4.0 2 0.010 1.0 6 0.030 3.0 0 0.000 0.0 
500         
 200 24 0.120 12.0 2 0.010 1.0 22 0.110 11.0 0 0.000 0.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 94 0.470 33.5 51 0.255 20.5 43 0.215 17.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 47 0.940 58.0 32 0.640 42.0 15 0.300 22.0 0 0.000 0.0
Trend: 4.163 0.400 4.312  
Probability: 0.000 0.345 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 08/21/1985    Harvest Time: 18 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.5 2 0.010 1.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
1000         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
1500         
 200 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
2000         
 200 6 0.030 2.5 3 0.015 1.5 3 0.015 1.5 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 293 1.465 77.5 98 0.490 39.5 185 0.925 60.0 10 0.050 1.0
Cyclophosphamide 
15         
 50 164 3.280 98.0 36 0.720 58.0 78 1.560 76.0 50 1.000 5.0
Trend: 0.899 0.847 0.901  
Probability: 0.184 0.198 0.184  


Trial #:3   Activation: No Activation    Date: 08/21/1985    Harvest Time: 22 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
250         
 200 4 0.020 2.0 1 0.005 0.5 3 0.015 1.5 0 0.000 0.0 
500         
 200 7 0.035 3.5 0 0.000 0.0 7 0.035 3.5 0 0.000 0.0 
1000         
 200 14 0.070 7.0 2 0.010 1.0 12 0.060 6.0 0 0.000 0.0 
1500         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 77 0.385 28.0 42 0.210 17.0 35 0.175 14.5 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 47 0.940 54.0 30 0.600 42.0 17 0.340 26.0 0 0.000 0.0
Trend: 3.130 1.293 2.852  
Probability: 0.001 0.098 0.002  


Trial #:4   Activation: No Activation    Date: 08/21/1985    Harvest Time: 17 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
250         
 200 15 0.075 7.5 0 0.000 0.0 15 0.075 7.5 0 0.000 0.0 
500         
 200 10 0.050 5.0 1 0.005 0.5 9 0.045 4.5 0 0.000 0.0 
1000         
 200 35 0.175 12.0 10 0.050 4.0 25 0.125 9.5 0 0.000 0.0 
1500         
 104 50 0.481 28.8 4 0.038 3.8 46 0.442 26.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 68 0.340 28.0 18 0.090 9.0 50 0.250 22.5 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 46 0.920 54.0 12 0.240 20.0 24 0.480 40.0 10 0.200 1.0
Trend: 7.108 4.012 6.302  
Probability: 0.000 0.000 0.000