| Study Attribute | Value | 
|---|---|
| CEBS Accession Number: | 002-01254-0015-0000-0 | 
| Chemical Name: | 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) | 
| CASRN: | 15481-70-6 | 
| NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity | 
| NTP Study ID: | 612221 | 
| Study Result: | Positive | 
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Water | 0 | 94 | 101 | 79 | 28 | 26 | 
| 105# | 105 | 85 | 27 | ||||
| 104 | 91 | 73 | 24 | ||||
| 86# | 102 | 64 | 25 | ||||
| Test Chemical | 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) | 125 | 106# | 97 | 82 | 26 | 31 | 
| 84 | 86 | 74 | 29 | ||||
| 63 | 79 | 72 | 38 | ||||
| 250 | 97# | 79 | 73 | 25 | 21 | ||
| 81 | 74 | 48 | 20 | ||||
| 98 | 81 | 54 | 18 | ||||
| 500 | 91 | 84 | 135 | 49 | 39* | ||
| 80# | 86 | 82 | 34 | ||||
| 80 | 73 | 78 | 33 | ||||
| 750 | 101 | 73 | 122 | 40 | 41* | ||
| 87# | 67 | 128 | 49 | ||||
| 104# | 70 | 100 | 32 | ||||
| 1000 | 83 | 32 | 223 | 90 | 76* | ||
| 76 | 43 | 161 | 71 | ||||
| 93 | 44 | 186 | 67 | ||||
| 1500 | |||||||
| Positive Control | Methyl Methane Sulfonate | 5 | 80 | 47 | 485 | 202 | 177* | 
| 86 | 58 | 459 | 179 | ||||
| 102 | 70 | 463 | 151 | ||||
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Water | 0 | 90 | 92 | 52 | 19 | 22 | 
| 60 | 65 | 50 | 28 | ||||
| 107 | 139 | 38 | 12 | ||||
| 83 | 104 | 69 | 28 | ||||
| Test Chemical | 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) | 200 | 38 | 48 | 34 | 30 | 41* | 
| 38 | 56 | 61 | 53 | ||||
| 41# | 46 | 50 | 41 | ||||
| 400 | 50# | 50 | 92 | 62 | 57* | ||
| 46# | 56 | 55 | 40 | ||||
| 56 | 45 | 116 | 69 | ||||
| 500 | 37# | 47 | 75 | 68 | 64* | ||
| 39 | 50 | 72 | 62 | ||||
| 41 | 33 | 76 | 62 | ||||
| 600 | 32 | 39 | 61 | 64 | 73* | ||
| 35 | 43 | 89 | 84 | ||||
| 51 | 49 | 107 | 70 | ||||
| 800 | 47# | 32 | 135 | 96 | 81* | ||
| 46# | 36 | 112 | 81 | ||||
| 51 | 42 | 101 | 66 | ||||
| Positive Control | Methyl Methane Sulfonate | 5 | 20# | 13 | 296 | 493 | 413* | 
| 20 | 12 | 319 | 532 | ||||
| 35 | 14 | 222 | 213 | ||||
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Water | 0 | 103 | 99 | 76 | 25 | 32 | 
| 95 | 97 | 107 | 37 | ||||
| 106 | 100 | 110 | 34 | ||||
| 106 | 104 | 94 | 30 | ||||
| Test Chemical | 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) | 500 | 74 | 49 | 126 | 57 | 57* | 
| 82 | 59 | 144 | 59 | ||||
| 86 | 53 | 142 | 55 | ||||
| 600 | 113 | 37 | 187 | 55 | 66* | ||
| 89 | 42 | 162 | 61 | ||||
| 77 | 40 | 189 | 82 | ||||
| 700 | 91 | 41 | 146 | 54 | 59* | ||
| 81 | 35 | 143 | 59 | ||||
| 75 | 28 | 146 | 65 | ||||
| 800 | 74 | 18 | 184 | 83 | 104* | ||
| 80 | 18 | 265 | 111 | ||||
| 60 | 19 | 214 | 120 | ||||
| 1000 | |||||||
| Positive Control | Methyl Methane Sulfonate | 5 | 59 | 44 | 414 | 235 | 308* | 
| 57# | 37 | 477 | 281 | ||||
| 49 | 31 | 600 | 410 | ||||
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination
 
                        