Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01254-0015-0000-0
Chemical Name:	2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride)
CASRN:	15481-70-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	612221
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	94	101	79	28	26
			105#	105	85	27
			104	91	73	24
			86#	102	64	25
Test Chemical	2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride)	125	106#	97	82	26	31
			84	86	74	29
			63	79	72	38
		250	97#	79	73	25	21
			81	74	48	20
			98	81	54	18
		500	91	84	135	49	39*
			80#	86	82	34
			80	73	78	33
		750	101	73	122	40	41*
			87#	67	128	49
			104#	70	100	32
		1000	83	32	223	90	76*
			76	43	161	71
			93	44	186	67
		1500


Positive Control	Methyl Methane Sulfonate	5	80	47	485	202	177*
			86	58	459	179
			102	70	463	151

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	90	92	52	19	22
			60	65	50	28
			107	139	38	12
			83	104	69	28
Test Chemical	2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride)	200	38	48	34	30	41*
			38	56	61	53
			41#	46	50	41
		400	50#	50	92	62	57*
			46#	56	55	40
			56	45	116	69
		500	37#	47	75	68	64*
			39	50	72	62
			41	33	76	62
		600	32	39	61	64	73*
			35	43	89	84
			51	49	107	70
		800	47#	32	135	96	81*
			46#	36	112	81
			51	42	101	66
Positive Control	Methyl Methane Sulfonate	5	20#	13	296	493	413*
			20	12	319	532
			35	14	222	213

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	103	99	76	25	32
			95	97	107	37
			106	100	110	34
			106	104	94	30
Test Chemical	2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride)	500	74	49	126	57	57*
			82	59	144	59
			86	53	142	55
		600	113	37	187	55	66*
			89	42	162	61
			77	40	189	82
		700	91	41	146	54	59*
			81	35	143	59
			75	28	146	65
		800	74	18	184	83	104*
			80	18	265	111
			60	19	214	120
		1000


Positive Control	Methyl Methane Sulfonate	5	59	44	414	235	308*
			57#	37	477	281
			49	31	600	410

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination