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Study Information
Study Attribute Value
CEBS Accession Number: 002-01254-0015-0000-0
Chemical Name: 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride)
CASRN: 15481-70-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 612221
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 94 101 79 28 26
    105# 105 85 27
    104 91 73 24
    86# 102 64 25
Test Chemical 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
125         
 106# 97 82 26 31
    84 86 74 29
    63 79 72 38
  
250         
 97# 79 73 25 21
    81 74 48 20
    98 81 54 18
  
500         
 91 84 135 49 39*
    80# 86 82 34
    80 73 78 33
  
750         
 101 73 122 40 41*
    87# 67 128 49
    104# 70 100 32
  
1000         
 83 32 223 90 76*
    76 43 161 71
    93 44 186 67
  
1500         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 80 47 485 202 177*
    86 58 459 179
    102 70 463 151

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 90 92 52 19 22
    60 65 50 28
    107 139 38 12
    83 104 69 28
Test Chemical 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
200         
 38 48 34 30 41*
    38 56 61 53
    41# 46 50 41
  
400         
 50# 50 92 62 57*
    46# 56 55 40
    56 45 116 69
  
500         
 37# 47 75 68 64*
    39 50 72 62
    41 33 76 62
  
600         
 32 39 61 64 73*
    35 43 89 84
    51 49 107 70
  
800         
 47# 32 135 96 81*
    46# 36 112 81
    51 42 101 66
Positive Control Methyl Methane Sulfonate 
5         
 20# 13 296 493 413*
    20 12 319 532
    35 14 222 213

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 103 99 76 25 32
    95 97 107 37
    106 100 110 34
    106 104 94 30
Test Chemical 2,6-Toluenediamine dihydrochloride (2,6-diaminotoluene dihydrochloride) 
500         
 74 49 126 57 57*
    82 59 144 59
    86 53 142 55
  
600         
 113 37 187 55 66*
    89 42 162 61
    77 40 189 82
  
700         
 91 41 146 54 59*
    81 35 143 59
    75 28 146 65
  
800         
 74 18 184 83 104*
    80 18 265 111
    60 19 214 120
  
1000         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 59 44 414 235 308*
    57# 37 477 281
    49 31 600 410

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination