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Study Information
Study Attribute Value
CEBS Accession Number: 002-01255-0001-0000-6
Chemical Name: 2,6-Xylidine
CASRN: 87-62-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 852885_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 05/23/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1032 483 0.468 9.66 25.5 0.000
Test Chemical 2,6-Xylidine 
33         
 50 1039 505 0.486 10.10 25.5 3.850 
98         
 50 1024 537 0.524 10.74 25.5 12.049 
327         
 50 1025 654 0.638 13.08 25.5 36.328
Positive Control Mitomycin-C 
 0.005     
 25 523 774 1.480 30.96 25.5 216.207
Trend: 5.438        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 07/22/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1032 420 0.407 8.40 25.7 0.000
Test Chemical 2,6-Xylidine 
301         
 50 1039 621 0.598 12.42 34.3 46.861 
348         
 50 1040 662 0.637 13.24 34.3 56.407 
400         
 50 1025 713 0.696 14.26 34.3 70.921 
451         
 0 0 0 0.000 0.00 34.3 N/A 
500         
 0 0 0 0.000 0.00 34.3 N/A
Positive Control Mitomycin-C 
 0.005     
 25 522 586 1.123 23.44 25.7 175.840
Trend: 8.408        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 05/23/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1033 468 0.453 9.36 25.8 0.000
Test Chemical 2,6-Xylidine 
33         
 50 1049 498 0.475 9.96 25.8 4.787 
98         
 0 0 0 0.000 0.00 0.0 N/A 
327         
 50 1033 437 0.423 8.74 25.8 -6.624 
980         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
1.5       
 25 519 867 1.671 34.68 25.8 268.728
Trend: -1.276        
Probability: 0.899        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 06/08/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1040 481 0.463 9.62 25.8 0.000
Test Chemical 2,6-Xylidine 
1000         
 50 1033 550 0.532 11.00 25.8 15.120 
1100         
 50 1027 571 0.556 11.42 25.8 20.214 
1200         
 50 1035 592 0.572 11.84 25.8 23.671
Positive Control Cyclophosphamide 
1.5       
 25 517 936 1.810 37.44 25.8 291.448
Trend: 3.488        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 07/22/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 397 0.380 7.94 25.7 0.000
Test Chemical 2,6-Xylidine 
1290         
 50 1044 578 0.554 11.56 34.3 45.731 
1390         
 50 1034 555 0.537 11.10 34.3 41.286 
1510         
 50 1037 574 0.554 11.48 34.3 45.700
Positive Control Cyclophosphamide 
1.5       
 25 521 686 1.317 27.44 25.7 246.587
Trend: 5.112        
Probability: 0.000