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Study Information
Study Attribute Value
CEBS Accession Number: 002-01257-0001-0000-8
Chemical Name: 2,7-Dinitro-9H-fluoren-9-one
CASRN: 31551-45-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 519842_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Questionable 
Induced Rat Liver S9  3_S9  Negative 
Induced Rat Liver S9  4_S9  Test Failure 
Induced Rat Liver S9  5_S9  Test Failure 

Trial #: 1    Activation: No Activation    Date: 10/18/1983    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1038 590 0.568 11.80 32.0 0.000
Test Chemical 2,7-Dinitro-9H-fluoren-9-one 
10         
 50 1039 767 0.738 15.34 25.8 29.875 
15         
 50 1050 843 0.803 16.86 25.8 41.248 
20         
 50 1042 896 0.860 17.92 32.0 51.281
Positive Control Mitomycin-C 
 0.0015    
 50 1043 717 0.687 14.34 25.8 20.943
Mitomycin-C 
 0.01      
 5 105 255 2.429 51.00 25.8 327.264
Trend: 7.881        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 01/17/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 524 344 0.656 13.76 32.3 0.000
Test Chemical 2,7-Dinitro-9H-fluoren-9-one 
10         
 25 524 488 0.931 19.52 26.0 41.860 
15         
 25 524 493 0.941 19.72 32.3 43.314 
20         
 25 519 548 1.056 21.92 32.3 60.837
Positive Control Mitomycin-C 
 0.0015    
 25 522 444 0.851 17.76 26.0 29.564
Mitomycin-C 
 0.01      
 5 105 302 2.876 60.40 26.0 338.117
Trend: 6.480        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/18/1983    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 448 0.430 8.96 25.8 0.000
Test Chemical 2,7-Dinitro-9H-fluoren-9-one 
10         
 50 1045 470 0.450 9.40 25.8 4.710 
15         
 50 1046 539 0.515 10.78 25.8 19.967 
20         
 50 1046 554 0.530 11.08 25.8 23.306
Positive Control Cyclophosphamide 
 0.4       
 50 1043 569 0.546 11.38 25.8 27.009
Cyclophosphamide 
2         
 5 104 114 1.096 22.80 25.8 155.198
Trend: 3.860        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 01/17/1984    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 596 0.568 11.92 26.0 0.000
Test Chemical 2,7-Dinitro-9H-fluoren-9-one 
10         
 50 1048 627 0.598 12.54 26.0 5.402 
15         
 50 1045 669 0.640 13.38 26.0 12.785 
20         
 50 1052 714 0.679 14.28 26.0 19.571
Positive Control Cyclophosphamide 
 0.4       
 50 1043 736 0.706 14.72 26.0 24.319
Cyclophosphamide 
2         
 5 105 174 1.657 34.80 26.0 191.946
Trend: 3.444        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 01/25/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 647 0.617 12.94 26.0 0.000
Test Chemical 2,7-Dinitro-9H-fluoren-9-one 
10         
 50 1034 718 0.694 14.36 26.0 12.476 
15         
 50 1043 693 0.664 13.86 26.0 7.623 
20         
 50 1040 728 0.700 14.56 26.0 13.385
Positive Control Cyclophosphamide 
 0.4       
 50 1042 837 0.803 16.74 26.0 30.111
Cyclophosphamide 
2         
 5 103 227 2.204 45.40 26.0 256.981
Trend: 1.881        
Probability: 0.030        

Trial #: 4_S9    Activation: Induced Rat Liver S9    Date: 02/15/1984    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1040 683 0.657 13.66 26.0 0.000
Negative (Not Specified) 
0         
 50 1050 630 0.600 12.60 26.0 -8.638
Test Chemical 2,7-Dinitro-9H-fluoren-9-one 
20         
 50 1048 732 0.698 14.64 26.0 6.356
Positive Control Cyclophosphamide 
 0.4       
 25 524 415 0.792 16.60 26.0 20.595
Cyclophosphamide 
2         
 5 105 208 1.981 41.60 26.0 201.638
Trend: 0.000        
Probability: 0.000        

Trial #: 5_S9    Activation: Induced Rat Liver S9    Date: 03/06/1984    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 525 220 0.419 8.80 26.0 0.000
Test Chemical 2,7-Dinitro-9H-fluoren-9-one 
20         
 25 525 288 0.549 11.52 26.0 30.909
Positive Control Cyclophosphamide 
 0.4       
 25 525 336 0.640 13.44 26.0 52.727
Cyclophosphamide 
2         
 5 105 188 1.790 37.60 26.0 327.273
Trend: 0.000        
Probability: 0.000