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Study Information
Study Attribute Value
CEBS Accession Number: 002-01265-0001-0000-7
Chemical Name: 2-Acetylaminofluorene
CASRN: 53-96-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 223461_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 01/06/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 392 0.373 7.84 26.0 0.000
Test Chemical 2-Acetylaminofluorene 
 0.5       
 50 1050 418 0.398 8.36 26.0 6.633 
1.6       
 50 1050 425 0.405 8.50 26.0 8.418 
5         
 50 1050 520 0.495 10.40 26.0 32.653 
16         
 50 1050 552 0.526 11.04 26.0 40.816 
50         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 513 0.489 10.26 26.0 30.867
Mitomycin-C 
 0.005     
 10 209 246 1.177 24.60 26.0 215.277
Trend: 6.208        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/06/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 376 0.360 7.52 26.0 0.000
Test Chemical 2-Acetylaminofluorene 
160         
 50 1046 950 0.908 19.00 26.0 151.935 
500         
 50 1046 954 0.912 19.08 26.0 152.996 
1600         
 50 1044 992 0.950 19.84 26.0 163.577 
2500         
 50 1046 1055 1.009 21.10 26.0 179.780
Positive Control Cyclophosphamide 
 0.1       
 50 1042 545 0.523 10.90 26.0 45.086
Cyclophosphamide 
 0.6       
 10 210 185 0.881 18.50 26.0 144.371
Trend: 15.049        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 03/24/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 495 0.471 9.90 26.0 0.000
Test Chemical 2-Acetylaminofluorene 
160         
 50 1049 997 0.950 19.94 26.0 101.606 
500         
 50 1050 887 0.845 17.74 26.0 79.192 
1600         
 50 1050 1247 1.188 24.94 26.0 151.919 
2500         
 50 1048 1347 1.285 26.94 26.0 172.641
Positive Control Cyclophosphamide 
 0.1       
 50 1050 661 0.630 13.22 26.0 33.535
Cyclophosphamide 
 0.6       
 10 210 243 1.157 24.30 26.0 145.455
Trend: 19.487        
Probability: 0.000