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Study Information
Study Attribute Value
CEBS Accession Number: 002-01265-0002-0000-8
Chemical Name: 2-Acetylaminofluorene
CASRN: 53-96-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 223461_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 04/01/1987    Harvest Time: 12.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: 2-Acetylaminofluorene 
300         
 200 81 0.405 26.0 31 0.155 14.5 50 0.250 16.5 0 0.000 0.0 
500         
 200 104 0.520 32.0 41 0.205 15.5 63 0.315 22.5 0 0.000 0.0 
1000         
 200 80 0.400 24.5 33 0.165 14.5 47 0.235 15.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 53 0.265 23.0 34 0.170 15.5 19 0.095 8.5 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 31 0.620 38.0 12 0.240 18.0 19 0.380 28.0 0 0.000 0.0
Trend: 5.768 4.402 4.221  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/01/1987    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: 2-Acetylaminofluorene 
1000         
 200 14 0.070 6.5 5 0.025 2.5 9 0.045 4.0 0 0.000 0.0 
1600         
 200 16 0.080 7.0 4 0.020 2.0 12 0.060 5.5 0 0.000 0.0 
2500         
 200 12 0.060 6.0 4 0.020 2.0 8 0.040 4.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
2.5       
 200 45 0.225 20.0 6 0.030 3.0 39 0.195 17.5 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 20 0.400 34.0 6 0.120 12.0 14 0.280 24.0 0 0.000 0.0
Trend: 2.475 1.377 2.077  
Probability: 0.007 0.084 0.019  


Trial #:2   Activation: No Activation    Date: 06/04/1987    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: 2-Acetylaminofluorene 
300         
 200 57 0.285 22.5 27 0.135 11.5 30 0.150 14.0 0 0.000 0.0 
500         
 200 77 0.385 28.5 38 0.190 16.5 39 0.195 14.5 0 0.000 0.0 
1000         
 200 77 0.385 29.5 28 0.140 13.0 49 0.245 20.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.0625    
 200 37 0.185 18.0 17 0.085 8.5 20 0.100 10.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 28 0.560 38.0 11 0.220 18.0 17 0.340 26.0 0 0.000 0.0
Trend: 7.219 4.450 5.702  
Probability: 0.000 0.000 0.000