Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01265-0003-0000-9
Chemical Name:	2-Acetylaminofluorene
CASRN:	53-96-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G09551
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	87	277	114	108
Vehicle Control			67	90	203	102	108
Test Chemical	2-Acetylaminofluorene	15.6	77	109	146	63	74
			74	93	189	85	74
		31.25	107	102	301	94	91
			112	98	295	88	91
		62.5	60	72	218	121	115
			76#	86	246	108	115
		125	81	63	306	126	115
			73	67	228	104	115
		250	67	67	239	118	116
			74	50	253	113	116
Positive Control	Ethyl Methane Sulfonate	500
Positive Control
Negative Control	Negative (Not Specified)	0	115	123	281	81
Negative Control

Nonactivation Trial: 2 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	112	19	10	12
Vehicle Control			68	122	29	14	12
Test Chemical	2-Acetylaminofluorene	15.6	82#	78	29	12	17
			45	58	30	22	17
		31.25	56	61	77	46	34*
			77	75	49	21	34*
		62.5	47	40	142	102	86*
			54	43	113	70	86*
		125	56	53	111	66	67*
			52	39	108	69	67*
		250	64	42	142	74	69*
			63	37	119	63	69*
Positive Control	Ethyl Methane Sulfonate	500	22	19	472	715	605*
Positive Control			26	19	389	496	605*
Negative Control	Negative (Not Specified)	0	53	65	24	15

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	91	88	49	18	20
Vehicle Control			101	98	69	23	20
Test Chemical	2-Acetylaminofluorene	15.6	80	89	54	23	24
			79	81	59	25	24
		31.25	79#	59	61.5	26	29
			77	86	75	32	29
		62.5	55	50	107	65	55*
			84	63	110	44	55*
		125	70	61	106	50	52*
			95	75	153	54	52*
		250	83	38	122	49	51*
			71	36	114	53	51*
Positive Control	Ethyl Methane Sulfonate	500	47	24	958	675	641*
Positive Control			56	31	1011	607	641*
Negative Control	Negative (Not Specified)	0	90	92	38	14	18*
Negative Control			89	121	58	22	18*

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	51	84	28	18	16
			49r	97	13	9
			65	115	32	16
			57	101	24	14
Test Chemical	2-Acetylaminofluorene	20	51	89	57	37	31*
			61	103	44	24	31*
		40	46#	65	105	76	68*
			43	86	76	59	68*
		50	45	58	152	112	102*
			47	70	129	91	102*
		60	60	54	188	105	104*
			40	48	123	102	104*
		80	52	31	214	138	132*
			32	30	121	125	132*
		100	47	47	142	101	104*
			54	49	173	107	104*
Positive Control	Ethyl Methane Sulfonate	500	27	24	402	493	606*
Positive Control			21	16	442	719	606*

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	81	31	16	19
			82	95	48	20
			61	80	36	20
			102	144	65	21
Test Chemical	2-Acetylaminofluorene	20	60	93	85	47	56*
			67	95	129	65	56*
		40	60	80	155	87	92*
			54	51	157	97	92*
		50	66	61	219	111	111*
			56	65	186	110	111*
		60	69	66	119	57	59*
			71	62	130	61	59*
		80	67	61	105	52	56*
			51	65	91	59	56*
		100	49	41	138	94	82*
			71	61	148	70	82*
Positive Control	Ethyl Methane Sulfonate	500	24	11	462	655	708*
			25	15	447	608
			20	11	516	860

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	115	83	32	27
Vehicle Control			92	112	63	23	27
Test Chemical	2-Acetylaminofluorene	50	106	64	116	37	45
			83	71	133	53	45
		75	64	53	141	73	70*
			84#	71	166.5	66	70*
		100	79	56	138	58	59*
			77	57	137	60	59*
		150	67	55	134	66	65*
			59	49	113	64	65*
		200	72	47	160	74	66*
			94	51	163	58	66*
Positive Control	3-Methylcholanthrene	7	70	68	280	134	141*
Positive Control			61	35	271	148	141*
Negative Control	Negative (Not Specified)	0	90	88	84	31	29*
Negative Control			78	85	65	28	29*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	66	105	54	61
Vehicle Control			76	65	157	69	61
Test Chemical	2-Acetylaminofluorene	20	61	18	198	108	109*
			68	19	224	110	109*
		30	61	14	391	213	188*
			61	18	298	162	188*
		40	48	17	276	192	205*
			49	13	322	218	205*
		60	44	15	251	192	208*
			39	14	265	225	208*
		100	45	5	366	273	305*
			27	2	271	337	305*
Positive Control	Dimethylnitrosoamine	100	16	4	145	296	335*
Positive Control			14	4	161	374	335*
Negative Control	Negative (Not Specified)	0	99	85	148	50	52*
Negative Control			89	184	147	55	52*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	70	150	68	62
Vehicle Control			91	97	150	55	62
Test Chemical	2-Acetylaminofluorene	15	87	14	288	111	133*
			85	16	394	155	133*
		30	67	10	394	196	188*
			68	10	371	181	188*
		60	50	8	375	253	246*
			45	8	322	239	246*
		80	30	4	252	277	269*
			31	3	238	260	269*
		100	20	2	220	364

		125

Positive Control	Dimethylnitrosoamine	100	20	3	193	327	348*
Positive Control			34	3	370	368	348*
Negative Control	Negative (Not Specified)	0	103	116	173	56	55*
Negative Control			92	116	148	54	55*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination