Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01265-0004-0000-0
Chemical Name:	2-Acetylaminofluorene
CASRN:	53-96-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G52274
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	92	110	234	85	96
Vehicle Control			81	90	261	108	96
Test Chemical	2-Acetylaminofluorene	31.3	56	49	229	138	140
			57	65	241	142	140
		62.5	43	32	228	177	204*
			48	42	332	232	204*
		125	48	31	377	260	250*
			48	29	344	240	250*
		250	21	9	400	640	599*
			24	11	399	558	599*
		500	22	4	303	463	488*
			21	9	318	513	488*
		1000	21#	10	459	717	665*
			19#	5	354	613	665*
Positive Control	Ethyl Methane Sulfonate	500	36	23	767	720	706*
Positive Control			41	28	859	693	706*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	92	125	58	51
Vehicle Control			76	108	100	44	51
Test Chemical	2-Acetylaminofluorene	31.3	78	100	116	49	51
			71	94	110	52	51
		62.5	62	71	246	133	145*
			65	79	306	157	145*
		125	63	63	339	180	156*
			65	63	259	133	156*
		250	36	22	340	318	426*
			28	19	444	535	426*
		500	33	17	306	312	367*
			28	14	359	422	367*
Positive Control	Ethyl Methane Sulfonate	500	27	26	543	662	541*
Positive Control			52#	49	661	420	541*

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	99	80	34	32
Vehicle Control			79	101	69	29	32
Test Chemical	2-Acetylaminofluorene	2.4	70	81	106	51	48
			78	93	107	46	48
		8.1	93	100	107	38	44
			74	94	113	51	44
		27	85	99	140	55	52
			72	90	105	48	52
		90	77	85	198	86	78*
			72	82	153	71	78*
		300	68	35	211	103	105*
			58	47	187	107	105*
		1000	64	50	187	97	102*
			69	28	222	107	102*
Positive Control	3-Methylcholanthrene	6	56	45	276	165	156*
Positive Control			55	49	242	146	156*

Non-Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	95	243	99	102
Vehicle Control			78	105	246	105	102
Test Chemical	2-Acetylaminofluorene	2.4	69	103	271	131	122
			72	108	245	113	122
		8.1	63#	94	236	126	124
			70	104	256	123	124
		27	74	109	281	126	134
			63	88	270	142	134
		90	63	87	336	178	172*
			58	72	290	165	172*
		300	62	55	312	167	172*
			60	71	321	177	172*
		1000	56	47	368	218	206*
			61	51	356	193	206*
Positive Control	3-Methylcholanthrene	6	55	54	360	218	226*
Positive Control			55	55	383	233	226*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75#	115	168	75	66
Vehicle Control			69	85	117	57	66
Test Chemical	2-Acetylaminofluorene	23.5	74	59	219	98	93
			70	60	184	88	93
		33.6	49	20	299	204	193*
			51	25	279	181	193*
		48	41	10	330	268	302*
			30	6	304	336	302*
		68.6	36	11	408	383	375*
			31	8	339	366	375*
		98	28	7	372	438	448*
			32	6	435	458	448*
		140	15	2	406	892	810*
			18	2	382	728	810*
		200

Positive Control	Dimethylnitrosoamine	100	14	15	141	332	349*
Positive Control			14	14	152	366	349*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	106	79	37	49
Vehicle Control			66	94	119	60	49
Test Chemical	2-Acetylaminofluorene	6.9	55	99	193	117	92*
			68	131	138	67	92*
		12.3	61	106	220	121	117*
			38	65	128	113	117*
		22	64	99	255	132	124*
			60	80	209	116	124*
		39.2	35	18	217	209	256*
			37	19	339	304	256*
		70	20	7	231	388	439*
			20	8	299	490	439*
Positive Control	3-Methylcholanthrene	5	43	44	257	199	218*
			39	48	277	238	218*
	3-Methylcholanthrene	100	1r	1	48	1371
			3r	2	45	562

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination