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Study Information
Study Attribute Value
CEBS Accession Number: 002-01277-0011-0000-1
Chemical Name: 2-Amino-4-nitrophenol
CASRN: 99-57-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 705388
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 111 101 122 37 43
    90 103 144 53
    75 101 94 42
    110 94 126 38
Test Chemical 2-Amino-4-nitrophenol 
25         
 70 53 198 94 98*
    61 53 209 114
    52 62 134 85
  
50         
 72 53 233 109 108*
    64 54 193 100
    61 50 211 115
  
100         
 65 27 349 179 194*
    54 20 320 198
    48 20 296 206
  
150         
 63 4 501 266 271*
    47 4 422 299
    50 10 370 248
  
200         
 273*
    46 4 366 267
    52 3 433 278
  
300         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 65 60 508 260 258*
    77 26 598 258
    65 60 503 257

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 93 90 98 35 29
    121r 126 92 25
    109 117 84 26
    111 93 89 27
Test Chemical 2-Amino-4-nitrophenol 
50         
 69 43 141 69 71*
    77 42 177 77
    93 51 192 69
  
75         
 114 57 251 74 73*
    79 44 165 70
    73 40 164 75
  
100         
 92 24 206 75 82*
    89 36 243 91
    87 32 208 80
  
150         
 93 17 365 131 118*
    86 20 280 108
    67 20 230 115
  
200         
 86 18 287 111 133*
    68 6 315 156
    91 10 360 133
  
300         
 100 8 393 131 143*
    67 5 295 148
    76 5 344 152
Positive Control Methyl Methane Sulfonate 
5         
 57 46 605 357 335*
    58 34 670 382
    75 62 592 265

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination