Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01277-0011-0000-1
Chemical Name:	2-Amino-4-nitrophenol
CASRN:	99-57-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	705388
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	111	101	122	37	43
			90	103	144	53
			75	101	94	42
			110	94	126	38
Test Chemical	2-Amino-4-nitrophenol	25	70	53	198	94	98*
			61	53	209	114
			52	62	134	85
		50	72	53	233	109	108*
			64	54	193	100
			61	50	211	115
		100	65	27	349	179	194*
			54	20	320	198
			48	20	296	206
		150	63	4	501	266	271*
			47	4	422	299
			50	10	370	248
		200					273*
			46	4	366	267
			52	3	433	278
		300


Positive Control	Methyl Methane Sulfonate	5	65	60	508	260	258*
			77	26	598	258
			65	60	503	257

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	90	98	35	29
			121r	126	92	25
			109	117	84	26
			111	93	89	27
Test Chemical	2-Amino-4-nitrophenol	50	69	43	141	69	71*
			77	42	177	77
			93	51	192	69
		75	114	57	251	74	73*
			79	44	165	70
			73	40	164	75
		100	92	24	206	75	82*
			89	36	243	91
			87	32	208	80
		150	93	17	365	131	118*
			86	20	280	108
			67	20	230	115
		200	86	18	287	111	133*
			68	6	315	156
			91	10	360	133
		300	100	8	393	131	143*
			67	5	295	148
			76	5	344	152
Positive Control	Methyl Methane Sulfonate	5	57	46	605	357	335*
			58	34	670	382
			75	62	592	265

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination