Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01283-0008-0000-4
Chemical Name:	2-Amino-5-nitrophenol
CASRN:	121-88-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	210466
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	99	76	31	31
			103	99	92	30
			85	102	84	33
Test Chemical	2-Amino-5-nitrophenol	12.5	73	88	131	60	61*
			71#	74	133	62	61*
		25	71	76	127	59	60*
			67#	79	124	62	60*
		50	69	46	160	77	76*
			63	44	143	76	76*
		100	61	28	152	84	77*
			73	24	153	70	77*
		200	50#	14	122	81	92*
			53	12	116	73
			61	7	222	122
		300	58	7	233	134	135*
			60	5	245	137	135*
		400


Positive Control	Methyl Methane Sulfonate	5	62	47	562	305	305*
Positive Control	Methyl Methane Sulfonate	10	33	12	517	520	520*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	112	88	76	23	31
			88	110	86	33
			90	98	92	34
			102	104	106	35
Test Chemical	2-Amino-5-nitrophenol	12.5	95	87	113	40	40
			95	87	95	33
			64	67	91	48
		25	96	52	158	55	65*
			81	69	177	73
			79	60	160	67
		50	94	41	184	65	80*
			66	35	216	109
			68	39	137	67
		100	73	24	142	65	69*
			70	29	131	63
			86	26	202	78
		200	89	12	212	79	86*
			75	12	192	85
			75	10	210	94
		300


Positive Control	Methyl Methane Sulfonate	5	71	68	347	163	163*
			86	72	412	160
			68	45	336	166

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination