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Study Information
Study Attribute Value
CEBS Accession Number: 002-01283-0008-0000-4
Chemical Name: 2-Amino-5-nitrophenol
CASRN: 121-88-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 210466
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 99 76 31 31
    103 99 92 30
    85 102 84 33
Test Chemical 2-Amino-5-nitrophenol 
12.5       
 73 88 131 60 61*
    71# 74 133 62
  
25         
 71 76 127 59 60*
    67# 79 124 62
  
50         
 69 46 160 77 76*
    63 44 143 76
  
100         
 61 28 152 84 77*
    73 24 153 70
  
200         
 50# 14 122 81 92*
    53 12 116 73
    61 7 222 122
  
300         
 58 7 233 134 135*
    60 5 245 137
  
400         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 62 47 562 305 305*
Methyl Methane Sulfonate 
10         
 33 12 517 520 520*

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 112 88 76 23 31
    88 110 86 33
    90 98 92 34
    102 104 106 35
Test Chemical 2-Amino-5-nitrophenol 
12.5       
 95 87 113 40 40
    95 87 95 33
    64 67 91 48
  
25         
 96 52 158 55 65*
    81 69 177 73
    79 60 160 67
  
50         
 94 41 184 65 80*
    66 35 216 109
    68 39 137 67
  
100         
 73 24 142 65 69*
    70 29 131 63
    86 26 202 78
  
200         
 89 12 212 79 86*
    75 12 192 85
    75 10 210 94
  
300         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 71 68 347 163 163*
    86 72 412 160
    68 45 336 166

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination