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Study Information
Study Attribute Value
CEBS Accession Number: 002-01284-0008-0000-5
Chemical Name: 2-Amino-5-nitrothiazole
CASRN: 121-66-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G59371
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 114 92 68 20 25
    73 89 67 30
    97 119 59 20
    96 100 81 28
Test Chemical 2-Amino-5-nitrothiazole 
62.5       
 52 7 313 202 191*
    56 13 305 180
  
125         
 50 5 354 236 216*
    59 8 348 196
  
250         
   
Positive Control Ethyl Methane Sulfonate 
500         
 15 6 446 991 927*
    18 9 457 862

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 103 101 73 24 30
    94 102 92 33
    89 96 92 35
Test Chemical 2-Amino-5-nitrothiazole 
15.6       
 100 32 328 110 126*
    87 29 372 143
  
31.3       
 92 25 439 160 166*
    95 22 494 173
  
62.5       
 83 23 382 154 169*
    75 16 411 184
  
125         
 77 32 347 151 153*
    85 34 397 156
  
250         
 65 19 395 202 176*
    76 30 343 151
  
500         
 93 22 505 181 246*
    64 12 598 310
Positive Control Ethyl Methane Sulfonate 
500         
 67 38 1112 553 479*
    78 53 946 404

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 81 107 55 23 23
    96 87 63 22
    83 110 53 21
    105 96 76 24
Test Chemical 2-Amino-5-nitrothiazole 
7.8       
 96 97 73 25 25
    103 99 75 24
  
15.6       
 97 95 75 26 28
    77 94 68 29
  
31.3       
 100 85 122 41 45*
    101 81 147 49
  
62.5       
 89 51 228 85 75*
    84 79 162 64
  
125         
 87 35 386 148 146*
    72 33 310 144
  
250         
 71 20 429 201 195*
    68 19 382 188
  
500         
 61 12 416 228 213*
    68 14 406 199
Positive Control Ethyl Methane Sulfonate 
500         
 43 24 796 612 907*
    25 13 840 1105
    27 16 823 1004

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 96 108 51 58
    65 87 100 51
    49r 80 139 95
    67 117 143 71
Test Chemical 2-Amino-5-nitrothiazole 
31.3       
 71 57 120 56 56
    57 57 96 56
  
62.5       
 79# 31 187.5 79 75
    52 22 112 71
  
125         
 74 24 145 66 81
    61 22 175 96
  
250         
 58 16 200 115 124*
    64 16 259 134
  
500         
 38 7 274 240 228*
    50 10 325 216
  
1000         
 35 5 519 490 562*
    29 3 548 634
Positive Control 3-Methylcholanthrene 
5         
 39 21 550 476 506*
    36 19 575 535

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73# 83 77 35 34
    84 66 89 35
    110 151 99 30
Test Chemical 2-Amino-5-nitrothiazole 
31.3       
 100 99 114 38 34
    80 77 73 31
  
62.5       
 71 72 71 33
  
125         
 70 45 119 57
  
250         
 89 40 139 52 48
    78 36 103 44
  
500         
 85 32 162 64 84*
    70 29 218 104
  
1000         
 71 21 352 165 175*
    67 18 372 185
Positive Control 3-Methylcholanthrene 
5         
 50 24 396 266 264*
    55 28 386 235
    43 28 380 292

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 105 81 38 42
    80 94 88 37
    86 114 70 27
    97 87 188 65
Test Chemical 2-Amino-5-nitrothiazole 
250         
 84 25 116 46 74*
    62 20 190 103
  
500         
 79 23 228 97 104*
    66 21 219 111
  
750         
 81 15 350 144 132*
    70 18 255 121
  
1000         
 65 14 644 332 319*
    60 11 548 306
  
1250         
 45 9 614 453 323*
    65 10 374 193
  
1500         
 38 5 527 468 364*
    47 5 363 260
Positive Control 3-Methylcholanthrene 
5         
 49 21 445 303 371*
    37 15 420 380
    37 15 477 432

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination