Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01284-0008-0000-5
Chemical Name:	2-Amino-5-nitrothiazole
CASRN:	121-66-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G59371
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	114	92	68	20	25
			73	89	67	30
			97	119	59	20
			96	100	81	28
Test Chemical	2-Amino-5-nitrothiazole	62.5	52	7	313	202	191*
			56	13	305	180	191*
		125	50	5	354	236	216*
			59	8	348	196	216*
		250

Positive Control	Ethyl Methane Sulfonate	500	15	6	446	991	927*
Positive Control			18	9	457	862	927*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	101	73	24	30
			94	102	92	33
			89	96	92	35
Test Chemical	2-Amino-5-nitrothiazole	15.6	100	32	328	110	126*
			87	29	372	143	126*
		31.3	92	25	439	160	166*
			95	22	494	173	166*
		62.5	83	23	382	154	169*
			75	16	411	184	169*
		125	77	32	347	151	153*
			85	34	397	156	153*
		250	65	19	395	202	176*
			76	30	343	151	176*
		500	93	22	505	181	246*
			64	12	598	310	246*
Positive Control	Ethyl Methane Sulfonate	500	67	38	1112	553	479*
Positive Control			78	53	946	404	479*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	107	55	23	23
			96	87	63	22
			83	110	53	21
			105	96	76	24
Test Chemical	2-Amino-5-nitrothiazole	7.8	96	97	73	25	25
			103	99	75	24	25
		15.6	97	95	75	26	28
			77	94	68	29	28
		31.3	100	85	122	41	45*
			101	81	147	49	45*
		62.5	89	51	228	85	75*
			84	79	162	64	75*
		125	87	35	386	148	146*
			72	33	310	144	146*
		250	71	20	429	201	195*
			68	19	382	188	195*
		500	61	12	416	228	213*
			68	14	406	199	213*
Positive Control	Ethyl Methane Sulfonate	500	43	24	796	612	907*
			25	13	840	1105
			27	16	823	1004

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	96	108	51	58
			65	87	100	51
			49r	80	139	95
			67	117	143	71
Test Chemical	2-Amino-5-nitrothiazole	31.3	71	57	120	56	56
			57	57	96	56	56
		62.5	79#	31	187.5	79	75
			52	22	112	71	75
		125	74	24	145	66	81
			61	22	175	96	81
		250	58	16	200	115	124*
			64	16	259	134	124*
		500	38	7	274	240	228*
			50	10	325	216	228*
		1000	35	5	519	490	562*
			29	3	548	634	562*
Positive Control	3-Methylcholanthrene	5	39	21	550	476	506*
Positive Control			36	19	575	535	506*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73#	83	77	35	34
			84	66	89	35
			110	151	99	30
Test Chemical	2-Amino-5-nitrothiazole	31.3	100	99	114	38	34
			80	77	73	31	34
		62.5	71	72	71	33
		125	70	45	119	57
		250	89	40	139	52	48
			78	36	103	44	48
		500	85	32	162	64	84*
			70	29	218	104	84*
		1000	71	21	352	165	175*
			67	18	372	185	175*
Positive Control	3-Methylcholanthrene	5	50	24	396	266	264*
			55	28	386	235
			43	28	380	292

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	105	81	38	42
			80	94	88	37
			86	114	70	27
			97	87	188	65
Test Chemical	2-Amino-5-nitrothiazole	250	84	25	116	46	74*
			62	20	190	103	74*
		500	79	23	228	97	104*
			66	21	219	111	104*
		750	81	15	350	144	132*
			70	18	255	121	132*
		1000	65	14	644	332	319*
			60	11	548	306	319*
		1250	45	9	614	453	323*
			65	10	374	193	323*
		1500	38	5	527	468	364*
			47	5	363	260	364*
Positive Control	3-Methylcholanthrene	5	49	21	445	303	371*
			37	15	420	380
			37	15	477	432

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination