Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01284-0009-0000-6
Chemical Name:	2-Amino-5-nitrothiazole
CASRN:	121-66-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G77278
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99	117	109	37	49
			83	94	159	64
			92	90	127	46
Test Chemical	2-Amino-5-nitrothiazole	96	71	35	399	188	185*
			69	34	376	182	185*
		192	70	32	362	172	206*
			62	30	448	240	206*
		384	48	16	489	337	283*
			56	19	384	229	283*
		480	51	17	470	310	286*
			48	15	381	263	286*
		600	48	11	464	320	352*
			42	8	479	385	352*
		750	32	4	513	537	524*
			33	4	503	511	524*
Positive Control	Ethyl Methane Sulfonate	500	51	38	1039	686	728*
			45	39	1090	813
			60	42	1227	685

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	110	95	139	42	39
			128r	107	167	44
			87	113	123	47
			80	92	64	27
Test Chemical	2-Amino-5-nitrothiazole	100	48	17	334	230	204*
			82	23	438	178	204*
		134	52	24	372	240	237*
			54	23	379	235	237*
		178	57	27	274	162	177*
			54	23	313	192	177*
		237	76	34	313	138	133*
			71	37	272	127	133*
		316	55	34	240	145	169*
			50	26	289	192	169*
		422	51	26	295	195	200*
			47	26	285	204	200*
		563	51	24	363	236	236*
			50	23	352	235	236*
		750	51	18	278	183	217*
			49	16	366	251	217*
Positive Control	Ethyl Methane Sulfonate	500	49	51	940	644	600*
			57	56	931	544
			50	47	917	611

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	70	101	170	81	76
			73	95	158	72
			66	95	164	83
			78	110	158	68
Test Chemical	2-Amino-5-nitrothiazole	63	65	59	186	96	81
			63	61	123	65	81
		79	75	57	174	78	74
			83	61	177	71	74
		98	68	34	190	93	80
			90	59	180	66	80
		123	63	38	200	106	97
			87	53	231	89	97
		153	62	33	215	117	108
			66	36	196	99	108
		192	66	31	254	129	112
			64	28	183	95	112
		240	70	33	286	136	130*
			68	32	250	123	130*
		300	55	17	293	179	195*
			48	14	303	210	195*
Positive Control	3-Methylcholanthrene	5	35#	28	532	514	395*
			49	24	515	349
			50	38	484	322

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	98	110	57	43
			69	100	82	40
			69	102	76	37
			74#	100	88.5	40
Test Chemical	2-Amino-5-nitrothiazole	74	73	81	171	78	69*
			70	81	126	60	69*
		106	57	58	115	67
		152	66	57	161	82	86*
			64	60	174	91	86*
		217	89#	52	208	78	84*
			64	40	172	90	84*
		255	53	32	206	130	127*
			62	39	232	124	127*
		300	61	37	165	90	99*
			52	30	167	107	99*
		353	58	31	173	99	100*
			54	29	164	101	100*
Positive Control	3-Methylcholanthrene	5	51	67	421	273	244*
			60	59	343	190
			53	69	427	270

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination