Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01294-0002-0000-0 |
Chemical Name: | 2-Biphenylamine hydrochloride |
CASRN: | 2185-92-4 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 404342_CA |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Questionable |
Induced Rat Liver S9 | 1_S9 | Negative |
No Activation | 2 | Negative |
No Activation | 3 | Weakly Positive |
No Activation | 4 | Negative |
No Activation | 5 | Weakly Positive* |
No Activation | 6 | Positive |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Biphenylamine hydrochloride | 50 | 100 | 4 | 0.040 | 4.0 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
75 | 100 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
100 | 100 | 8 | 0.080 | 7.0 | 4 | 0.040 | 4.0 | 4 | 0.040 | 3.0 | 0 | 0.000 | 0.0 | ||
150 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.125 | 100 | 23 | 0.230 | 19.0 | 15 | 0.150 | 14.0 | 8 | 0.080 | 8.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 15 | 0.300 | 22.0 | 11 | 0.220 | 18.0 | 4 | 0.080 | 8.0 | 0 | 0.000 | 0.0 | |
Trend: | 1.937 | 1.318 | 1.420 | ||||||||||||
Probability: | 0.026 | 0.094 | 0.078 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Biphenylamine hydrochloride | 75 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
100 | 100 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | ||
150 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
200 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 10 | 100 | 33 | 0.330 | 31.0 | 23 | 0.230 | 22.0 | 10 | 0.100 | 10.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 15 | 50 | 30 | 0.600 | 50.0 | 23 | 0.460 | 40.0 | 7 | 0.140 | 14.0 | 0 | 0.000 | 0.0 | |
Trend: | 1.117 | 0.857 | 0.757 | ||||||||||||
Probability: | 0.132 | 0.196 | 0.225 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 3 | 0.015 | 2.0 | 0 | 0.000 | 0.0 | 3 | 0.015 | 2.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Biphenylamine hydrochloride | 50 | 200 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
100 | 200 | 7 | 0.035 | 4.0 | 0 | 0.000 | 0.0 | 7 | 0.035 | 4.0 | 0 | 0.000 | 0.0 | ||
150 | 200 | 7 | 0.035 | 4.0 | 1 | 0.005 | 1.0 | 6 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | ||
200 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.063 | 200 | 114 | 0.570 | 36.0 | 56 | 0.280 | 21.0 | 58 | 0.290 | 24.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.125 | 50 | 38 | 0.760 | 50.0 | 18 | 0.360 | 32.0 | 20 | 0.400 | 34.0 | 0 | 0.000 | 0.0 | |
Trend: | 1.852 | 1.796 | 1.365 | ||||||||||||
Probability: | 0.032 | 0.036 | 0.086 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 2 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Biphenylamine hydrochloride | 50 | 200 | 3 | 0.015 | 2.0 | 0 | 0.000 | 0.0 | 3 | 0.015 | 2.0 | 0 | 0.000 | 0.0 |
100 | 200 | 3 | 0.015 | 2.0 | 0 | 0.000 | 0.0 | 3 | 0.015 | 2.0 | 0 | 0.000 | 0.0 | ||
150 | 200 | 12 | 0.060 | 5.0 | 0 | 0.000 | 0.0 | 12 | 0.060 | 5.0 | 0 | 0.000 | 0.0 | ||
200 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.063 | 200 | 29 | 0.145 | 14.0 | 9 | 0.045 | 4.0 | 20 | 0.100 | 10.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.125 | 50 | 16 | 0.320 | 28.0 | 5 | 0.100 | 10.0 | 11 | 0.220 | 22.0 | 0 | 0.000 | 0.0 | |
Trend: | 2.320 | 0.000 | 2.320 | ||||||||||||
Probability: | 0.010 | 0.000 | 0.010 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Biphenylamine hydrochloride | 50 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
75 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
100 | 100 | 5 | 0.050 | 5.0 | 0 | 0.000 | 0.0 | 5 | 0.050 | 5.0 | 0 | 0.000 | 0.0 | ||
150 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
200 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.063 | 100 | 55 | 0.550 | 40.0 | 30 | 0.300 | 24.0 | 25 | 0.250 | 22.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.125 | 50 | 55 | 1.100 | 58.0 | 30 | 0.600 | 44.0 | 25 | 0.500 | 32.0 | 0 | 0.000 | 0.0 | |
Trend: | -0.160 | 0.000 | 0.435 | ||||||||||||
Probability: | 0.564 | 0.000 | 0.332 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Biphenylamine hydrochloride | 75 | 100 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
100 | 100 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
150 | 100 | 4 | 0.040 | 4.0 | 1 | 0.010 | 1.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | ||
200 | 100 | 19 | 0.190 | 11.0 | 8 | 0.080 | 6.0 | 11 | 0.110 | 6.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.125 | 100 | 13 | 0.130 | 13.0 | 7 | 0.070 | 7.0 | 6 | 0.060 | 6.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 13 | 0.260 | 18.0 | 6 | 0.120 | 10.0 | 7 | 0.140 | 12.0 | 0 | 0.000 | 0.0 | |
Trend: | 3.701 | 2.610 | 2.879 | ||||||||||||
Probability: | 0.000 | 0.005 | 0.002 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Biphenylamine hydrochloride | 100 | 100 | 5 | 0.050 | 5.0 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
150 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
200 | 100 | 6 | 0.060 | 6.0 | 0 | 0.000 | 0.0 | 6 | 0.060 | 6.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.125 | 100 | 13 | 0.130 | 13.0 | 7 | 0.070 | 7.0 | 6 | 0.060 | 6.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 13 | 0.260 | 18.0 | 6 | 0.120 | 10.0 | 7 | 0.140 | 12.0 | 0 | 0.000 | 0.0 | |
Trend: | 1.714 | -0.441 | 2.401 | ||||||||||||
Probability: | 0.043 | 0.670 | 0.008 |