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Study Information
Study Attribute Value
CEBS Accession Number: 002-01294-0002-0000-0
Chemical Name: 2-Biphenylamine hydrochloride
CASRN: 2185-92-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 404342_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Questionable 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
No Activation  3  Weakly Positive 
No Activation  4  Negative 
No Activation  5  Weakly Positive* 
No Activation  6  Positive 

Trial #:1   Activation: No Activation    Date: 06/05/1985    Harvest Time: 12.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2-Biphenylamine hydrochloride 
50         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
75         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
100         
 100 8 0.080 7.0 4 0.040 4.0 4 0.040 3.0 0 0.000 0.0 
150         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 23 0.230 19.0 15 0.150 14.0 8 0.080 8.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 15 0.300 22.0 11 0.220 18.0 4 0.080 8.0 0 0.000 0.0
Trend: 1.937 1.318 1.420  
Probability: 0.026 0.094 0.078  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 03/19/1985    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2-Biphenylamine hydrochloride 
75         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
100         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0 
150         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
200         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 100 33 0.330 31.0 23 0.230 22.0 10 0.100 10.0 0 0.000 0.0
Cyclophosphamide 
15         
 50 30 0.600 50.0 23 0.460 40.0 7 0.140 14.0 0 0.000 0.0
Trend: 1.117 0.857 0.757  
Probability: 0.132 0.196 0.225  


Trial #:2   Activation: No Activation    Date: 10/16/1985    Harvest Time: 17.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 2.0 0 0.000 0.0 3 0.015 2.0 0 0.000 0.0
Test Chemical: 2-Biphenylamine hydrochloride 
50         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
100         
 200 7 0.035 4.0 0 0.000 0.0 7 0.035 4.0 0 0.000 0.0 
150         
 200 7 0.035 4.0 1 0.005 1.0 6 0.030 3.0 0 0.000 0.0 
200         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 114 0.570 36.0 56 0.280 21.0 58 0.290 24.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 38 0.760 50.0 18 0.360 32.0 20 0.400 34.0 0 0.000 0.0
Trend: 1.852 1.796 1.365  
Probability: 0.032 0.036 0.086  


Trial #:3   Activation: No Activation    Date: 10/16/1985    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: 2-Biphenylamine hydrochloride 
50         
 200 3 0.015 2.0 0 0.000 0.0 3 0.015 2.0 0 0.000 0.0 
100         
 200 3 0.015 2.0 0 0.000 0.0 3 0.015 2.0 0 0.000 0.0 
150         
 200 12 0.060 5.0 0 0.000 0.0 12 0.060 5.0 0 0.000 0.0 
200         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 29 0.145 14.0 9 0.045 4.0 20 0.100 10.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 16 0.320 28.0 5 0.100 10.0 11 0.220 22.0 0 0.000 0.0
Trend: 2.320 0.000 2.320  
Probability: 0.010 0.000 0.010  


Trial #:4   Activation: No Activation    Date: 07/17/1985    Harvest Time: 18 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2-Biphenylamine hydrochloride 
50         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
75         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
100         
 100 5 0.050 5.0 0 0.000 0.0 5 0.050 5.0 0 0.000 0.0 
150         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
200         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 100 55 0.550 40.0 30 0.300 24.0 25 0.250 22.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 55 1.100 58.0 30 0.600 44.0 25 0.500 32.0 0 0.000 0.0
Trend: -0.160 0.000 0.435  
Probability: 0.564 0.000 0.332  


Trial #:5   Activation: No Activation    Date: 03/19/1985    Harvest Time: 13 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2-Biphenylamine hydrochloride 
75         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
100         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
150         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
200         
 100 19 0.190 11.0 8 0.080 6.0 11 0.110 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 13 0.130 13.0 7 0.070 7.0 6 0.060 6.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 13 0.260 18.0 6 0.120 10.0 7 0.140 12.0 0 0.000 0.0
Trend: 3.701 2.610 2.879  
Probability: 0.000 0.005 0.002  


Trial #:6   Activation: No Activation    Date: 03/19/1985    Harvest Time: 18 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2-Biphenylamine hydrochloride 
100         
 100 5 0.050 5.0 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 
150         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
200         
 100 6 0.060 6.0 0 0.000 0.0 6 0.060 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 13 0.130 13.0 7 0.070 7.0 6 0.060 6.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 13 0.260 18.0 6 0.120 10.0 7 0.140 12.0 0 0.000 0.0
Trend: 1.714 -0.441 2.401  
Probability: 0.043 0.670 0.008