Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01294-0023-0000-3
Chemical Name:	2-Biphenylamine hydrochloride
CASRN:	2185-92-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	092229
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	86	137	70	52
			79	84	129	55
			81	106	119	49
			79	124	85	36
Test Chemical	2-Biphenylamine hydrochloride	12.5	73	81	193	89	64
			96	93	113	39	64
		25	70	81	127	60	62
			68	36	130	64	62
		50	75	90	98	44	57
			55	65	117	71	57
		100	53	45	89	56	53
			58	43	86	50	53
		200	72	11	143	66	67
			57	11	117	69	67
Positive Control	Methyl Methane Sulfonate	15	26	24	258	333	306*
Positive Control			27	25	225	280	306*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	98	29	11	14
			94	105	34	12
			82	96	44	18
			97	102	44	15
Test Chemical	2-Biphenylamine hydrochloride	20	103	103	58	19	19
			101	97	60	20	19
		60	93	74	55	20	18
			92	71	46	17	18
		100	105	54	47	15	17
			88	50	48	18	17
		140	132r	41	104	26
			117	30	67	19
		180

Positive Control	Methyl Methane Sulfonate	15	57	30	178	104	117*
Positive Control			35	26	136	129	117*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	100	50	22	23
			72	96	42	19
			74	95	59	27
			78	110	55	24
Test Chemical	2-Biphenylamine hydrochloride	80	71	70	72	34	31
			65	57	56	29	31
		110	48	34	55	38	38*
			58	44	65	37	38*
		140	62	39	50	27	29
			64	41	61	32	29
		170	69	25	73	35	41*
			54	21	77	47	41*
		200	61	17	68	37	40*
			65	18	81	42	40*
Positive Control	3-Methylcholanthrene	2.5	46	39	294	213	205*
Positive Control			50	41	293	196	205*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	95	40	20	23
			69	114	35	17
			74	111	63	29
			52	80	43	28
Test Chemical	2-Biphenylamine hydrochloride	60	95	68	44	15	19
			117	70	79	22	19
		90	119	65	53	15
			127r	60	69	18
		120	77	40	88	38	46*
			87	46	143	55	46*
		150	78	32	144	61	49*
			91	43	99	36	49*
		180	77	21	122	53	50*
			92	21	127	46	50*
		210

Positive Control	3-Methylcholanthrene	2.5	48	31	280	196	280*
Positive Control			34	24	368	364	280*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	92	167	70	71
			76	94	174	76
			92	124	158	57
			74	89	176	79
Test Chemical	2-Biphenylamine hydrochloride	120	61	31	90	49	54
			68	33	122	60	54
		140	60	30	121	68	63
			60	27	105	58	63
		160	65	25	137	70	70
			77	20	163	71	70
		180	70	15	210	100	91
			74	18	183	82	91
		200	56	10	261	154	123*
			53	10	145	91	123*
Positive Control	3-Methylcholanthrene	2.5	44	31	244	185	160*
Positive Control			40	33	162	134	160*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination