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Study Information
Study Attribute Value
CEBS Accession Number: 002-01304-0001-0000-1
Chemical Name: 2-Butoxyethanol (ethylene glycol monobutyl ether)
CASRN: 111-76-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 029931_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Test Failure 
No Activation  2  Questionable 
No Activation  3  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 10/22/1985    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1017 491 0.483 9.82 26.0 0.000
Test Chemical 2-Butoxyethanol (ethylene glycol monobutyl ether) 
167         
 50 1013 473 0.467 9.46 26.0 -3.286 
500         
 50 1026 488 0.476 9.76 26.0 -1.483 
1670         
 50 1024 474 0.463 9.48 26.0 -4.122 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1005 576 0.573 11.52 26.0 18.712
Mitomycin-C 
 0.01      
 5 103 144 1.398 28.80 26.0 189.577
Trend: -0.535        
Probability: 0.704        

Trial #: 2    Activation: No Activation    Date: 01/07/1986    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1016 418 0.411 8.36 26.0 0.000
Test Chemical 2-Butoxyethanol (ethylene glycol monobutyl ether) 
1510         
 50 1004 410 0.408 8.20 31.0 -0.742 
2220         
 50 998 453 0.454 9.06 31.0 10.328 
3000         
 50 1013 496 0.490 9.92 31.0 19.012 
4000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1017 568 0.559 11.36 26.0 35.752
Mitomycin-C 
 0.01      
 5 103 150 1.456 30.00 26.0 253.974
Trend: 3.010        
Probability: 0.001        

Trial #: 3    Activation: No Activation    Date: 02/04/1986    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1027 485 0.472 9.70 26.0 0.000
Test Chemical 2-Butoxyethanol (ethylene glycol monobutyl ether) 
2500         
 50 1007 531 0.527 10.62 36.0 11.659 
3000         
 50 1009 541 0.536 10.82 36.0 13.536 
3500         
 50 1007 551 0.547 11.02 36.0 15.865 
4000         
 0 0 0 0.000 0.00 36.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1015 626 0.617 12.52 26.0 30.598
Mitomycin-C 
 0.01      
 5 102 202 1.980 40.40 26.0 319.353
Trend: 2.303        
Probability: 0.011        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/22/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1006 491 0.488 9.82 26.0 0.000
Test Chemical 2-Butoxyethanol (ethylene glycol monobutyl ether) 
500         
 50 1019 485 0.476 9.70 26.0 -2.482 
1670         
 50 1015 479 0.472 9.58 26.0 -3.309 
5000         
 50 1026 497 0.484 9.94 26.0 -0.751
Positive Control Cyclophosphamide 
 0.4       
 50 1038 705 0.679 14.10 26.0 39.158
Cyclophosphamide 
2         
 5 102 128 1.255 25.60 26.0 157.114
Trend: -0.158        
Probability: 0.563