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Study Information
Study Attribute Value
CEBS Accession Number: 002-01308-0008-0000-2
Chemical Name: 2-Chloro-p-phenylenediamine sulfate
CASRN: 61702-44-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 962361
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 58# 86 79 45 27
    76 82 70 31
    113 116 53 16
    107 115 46 14
Test Chemical 2-Chloro-p-phenylenediamine sulfate 
1.25      
 63 50 76 40 28
    120r 48 95 26
    102 86 47 15
  
2.5       
 66 56 71 36 39
    71 61 94 44
    80 53 91 38
  
5         
 65 46 113 58 52*
    83 56 152 61
    80 70 90 38
  
7.5       
 72 41 107 50 41*
    106 63 106 33
    78 57 91 39
  
10         
 94 53 87 31 31
    87# 39 75 29
    106 61 104 33
  
15         
 84 13 125 50 48*
    72 16 88 41
    68 13 108 53
Positive Control Methyl Methane Sulfonate 
5         
 61# 40 470 255 252*
    68 39 554 271
    60 34 412 231

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 113 94 73 22 24
    113 92 66 19
    112 115 97 29
    82 100 65 26
Test Chemical 2-Chloro-p-phenylenediamine sulfate 
2.5       
 62 60 67 36 32
    81 58 76 31
    117 60 101 29
  
5         
 94 55 80 28 35
    98 57 78 27
    69 71 101 49
  
7.5       
 104 67 87 28 37*
    82 52 102 41
    89 47 110 41
  
10         
 79 44 131 56 50*
    74 51 103 47
    84 37 123 49
  
15         
 66 29 138 69 65*
    79 31 147 62
    83 33 157 63
  
20         
 108 21 219 67 107*
    91 20 400 146
    147r 47 425 96
  
30         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 57 36 743 433 423*
    57 36 674 394
    57 36 759 443

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 102 129 129 42 42
    116 131 117 34
    124r 174 160 43
    93 41 141 51
Test Chemical 2-Chloro-p-phenylenediamine sulfate 
2.5       
 111 75 144 43 43
    101 77 131 43
  
5         
 120r 65 127 35
    122r 67 159 43
    103 68 201 65
  
7.5       
 118 70 150 42 49
    120r 59 192 53
    117 49 192 55
  
10         
 116 47 237 68 56
    96 66 128 44
    123r 54 262 71
  
15         
 124r 33 336 90
    120r 52 423 117
    119# 23 499 140
  
20         
 74 4 422 189 163*
    99 12 348 118
    96 5 524 181
  
30         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 87# 60 621 238 298*
    83 57 781 315
    75 35 760 339

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 117 117 96 27 48
    123r 92 77 21
    83 94 118 48
    65 89 136 70
Test Chemical 2-Chloro-p-phenylenediamine sulfate 
5         
 115 87 180 52 56
    106 86 190 60
  
7.5       
 103 81 171 55 49
    100 78 140 47
    120 96 164 46
  
10         
 78 58 168 71 60
    90 59 166 61
    112 76 155 46
  
15         
 109 68 203 62 65
    102 76 233 76
    116 65 200 58
  
20         
 97 56 167 57 60
    104# 80 175 56
    101 71 201 67
  
30         
 112 55 200 60 109*
    103 52 196 63
    27 11 163 204
Positive Control Methyl Methane Sulfonate 
5         
 86# 50 701 272 314*
    71 58 743 350
    87 51 831 320

Nonactivation Trial: 5 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 113 123 47 56
    70 92 153 73
    60 112 94 53
    67# 84 101 50
Test Chemical 2-Chloro-p-phenylenediamine sulfate 
7.5       
 111 81 222 67 49
    117 108 194 55
    111 95 85 25
  
10         
 86 51 256 99 125*
    83 34 371 150
    123r 52 335 91
  
15         
 101 25 583 193 240*
    43 3 428 336
    76 5 438 191
  
20         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 90 71 1061 394 391*
    67 60 772 387
    77 98 904 392

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination