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Study Information
Study Attribute Value
CEBS Accession Number: 002-01313-0003-0000-3
Chemical Name: 2-Chloroethyltrimethylammonium chloride
CASRN: 999-81-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 564198_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Test Failure 
No Activation  2  Positive 
No Activation  3  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 03/12/1985    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1023 443 0.433 8.86 26.0 0.000
Test Chemical 2-Chloroethyltrimethylammonium chloride 
500         
 50 996 397 0.399 7.94 26.0 -7.954 
1670         
 50 1023 441 0.431 8.82 26.0 -0.451 
5000         
 50 1024 437 0.427 8.74 26.0 -1.451
Positive Control Mitomycin-C 
 0.001     
 50 1031 526 0.510 10.52 26.0 17.815
Mitomycin-C 
 0.01      
 5 105 207 1.971 41.40 26.0 355.253
Trend: 0.152        
Probability: 0.440        

Trial #: 2    Activation: No Activation    Date: 10/15/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1040 401 0.386 8.02 26.0 0.000
Test Chemical 2-Chloroethyltrimethylammonium chloride 
1675         
 50 1028 486 0.473 9.72 26.0 22.612 
3350         
 50 1022 529 0.518 10.58 26.0 34.244 
5000         
 50 1030 502 0.487 10.04 26.0 26.402
Positive Control Mitomycin-C 
 0.001     
 50 1011 577 0.571 11.54 26.0 48.018
Mitomycin-C 
 0.01      
 5 104 185 1.779 37.00 26.0 361.347
Trend: 3.866        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 10/24/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1010 421 0.417 8.42 26.0 0.000
Test Chemical 2-Chloroethyltrimethylammonium chloride 
1675         
 50 1013 468 0.462 9.36 26.0 10.835 
3350         
 50 1033 412 0.399 8.24 26.0 -4.317 
5000         
 50 1029 450 0.437 9.00 26.0 4.915
Positive Control Mitomycin-C 
 0.001     
 50 1017 522 0.513 10.44 26.0 23.137
Mitomycin-C 
 0.01      
 5 104 161 1.548 32.20 26.0 271.391
Trend: -0.192        
Probability: 0.576        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 03/12/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1037 433 0.418 8.66 26.0 0.000
Test Chemical 2-Chloroethyltrimethylammonium chloride 
500         
 50 1010 397 0.393 7.94 26.0 -5.863 
1700         
 50 1034 399 0.386 7.98 26.0 -7.585 
5000         
 50 1028 425 0.413 8.50 26.0 -0.988
Positive Control Cyclophosphamide 
 0.4       
 50 1018 608 0.597 12.16 26.0 43.036
Cyclophosphamide 
2         
 5 104 200 1.923 40.00 26.0 360.561
Trend: -0.250        
Probability: 0.599