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Study Information
Study Attribute Value
CEBS Accession Number: 002-01313-0011-0000-2
Chemical Name: 2-Chloroethyltrimethylammonium chloride
CASRN: 999-81-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 899913
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 92 64 63 23 21
    106 98 52 16
    113 117 65 19
    108 121 82 25
Test Chemical 2-Chloroethyltrimethylammonium chloride 
500         
 99 73 74 25 24
    115 50 83 24
    110 64 76 23
  
1000         
 123r 120 81 22 23
    114 87 95 28
    114 130 61 18
  
2000         
 112 123 87 26 26
    97 55 79 27
    79 65 62 26
  
3000         
 106 94 99 31 28
    121r 93 101 28
    109 143 82 25
  
4000         
 114 86 90 26 24
    103# 29 60 19
    109 61 83 25
  
5000         
 99 44 74 25 22
    92 6 57 21
    118 67 72 20
Positive Control Methyl Methane Sulfonate 
5         
 117*
    108 74 264 81
    78 16 359 153

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 109 123 210 65 54
    121r 131 182 50
    107 79 168 52
    110 98 145 44
Test Chemical 2-Chloroethyltrimethylammonium chloride 
500         
 108 100 130 40 40
    109 112 127 39
    112 124 136 40
  
1000         
 128r 134 79 21 42
    109 107 151 46
    117 118 131 37
  
2000         
 104 103 157 50 51
    103 118 158 51
    75# 84 116 52
  
3000         
 113 127 158 47 49
    100 112 161 54
    105 109 149 47
  
4000         
 105 77 162 51 56
    107 90 191 59
    103 93 174 57
  
5000         
 117 112 133 38 42
    106 132 165 52
    117 115 128 36
Positive Control Methyl Methane Sulfonate 
5         
 107 60 896 279 366*
    66 44 916 463
    82 53 878 355

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 94 74 83 29 30
    112 119 101 30
    119 98 112 31
    86 109 71 28
Test Chemical 2-Chloroethyltrimethylammonium chloride 
500         
 118 104 83 23 26
    104 85 92 30
    106 98 84 26
  
1000         
 116 109 85 25 28
    126r 102 98 26
    99 85 92 31
  
2000         
 87 89 87 33 29
    109 111 102 31
    116 101 82 24
  
3000         
 109 90 100 31 32
    116 131 114 33
    115 77 109 32
  
4000         
 123r 93 155 42
    129r 83 126 33
    114# 77 144 42
  
5000         
 112 68 126 38 33
    107 93 90 28
    97 89 98 34
Positive Control 3-Methylcholanthrene 
2.5       
 89 39 484 182 328*
    65 27 852 440
    76 33 825 361

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 112 63 80 24 28
    109 129 109 33
    112 119 78 23
    107 106 95 30
Test Chemical 2-Chloroethyltrimethylammonium chloride 
500         
 114 84 90 26 24
    118 142 72 20
    102 159 78 25
  
1000         
 141r 66 67 16
    113 113 102 30
    121r 66 82 23
  
2000         
 109 103 67 21 27
    109 115 123 38
    115 83 80 23
  
3000         
 120 111 79 22 24
    100 84 76 25
    115 108 85 25
  
4000         
 111 79 74 22 25
    102 62 74 24
    105 94 86 27
  
5000         
 111 64 143 43 33
    110 65 91 28
    108 66 89 27
Positive Control 3-Methylcholanthrene 
2.5       
 70 15 527 250 259*
    105 41 1008 320
    107 55 669 209

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination