Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01313-0011-0000-2
Chemical Name:	2-Chloroethyltrimethylammonium chloride
CASRN:	999-81-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	899913
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	92	64	63	23	21
			106	98	52	16
			113	117	65	19
			108	121	82	25
Test Chemical	2-Chloroethyltrimethylammonium chloride	500	99	73	74	25	24
			115	50	83	24
			110	64	76	23
		1000	123r	120	81	22	23
			114	87	95	28
			114	130	61	18
		2000	112	123	87	26	26
			97	55	79	27
			79	65	62	26
		3000	106	94	99	31	28
			121r	93	101	28
			109	143	82	25
		4000	114	86	90	26	24
			103#	29	60	19
			109	61	83	25
		5000	99	44	74	25	22
			92	6	57	21
			118	67	72	20
Positive Control	Methyl Methane Sulfonate	5					117*
			108	74	264	81
			78	16	359	153

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	109	123	210	65	54
			121r	131	182	50
			107	79	168	52
			110	98	145	44
Test Chemical	2-Chloroethyltrimethylammonium chloride	500	108	100	130	40	40
			109	112	127	39
			112	124	136	40
		1000	128r	134	79	21	42
			109	107	151	46
			117	118	131	37
		2000	104	103	157	50	51
			103	118	158	51
			75#	84	116	52
		3000	113	127	158	47	49
			100	112	161	54
			105	109	149	47
		4000	105	77	162	51	56
			107	90	191	59
			103	93	174	57
		5000	117	112	133	38	42
			106	132	165	52
			117	115	128	36
Positive Control	Methyl Methane Sulfonate	5	107	60	896	279	366*
			66	44	916	463
			82	53	878	355

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	94	74	83	29	30
			112	119	101	30
			119	98	112	31
			86	109	71	28
Test Chemical	2-Chloroethyltrimethylammonium chloride	500	118	104	83	23	26
			104	85	92	30
			106	98	84	26
		1000	116	109	85	25	28
			126r	102	98	26
			99	85	92	31
		2000	87	89	87	33	29
			109	111	102	31
			116	101	82	24
		3000	109	90	100	31	32
			116	131	114	33
			115	77	109	32
		4000	123r	93	155	42
			129r	83	126	33
			114#	77	144	42
		5000	112	68	126	38	33
			107	93	90	28
			97	89	98	34
Positive Control	3-Methylcholanthrene	2.5	89	39	484	182	328*
			65	27	852	440
			76	33	825	361

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	112	63	80	24	28
			109	129	109	33
			112	119	78	23
			107	106	95	30
Test Chemical	2-Chloroethyltrimethylammonium chloride	500	114	84	90	26	24
			118	142	72	20
			102	159	78	25
		1000	141r	66	67	16
			113	113	102	30
			121r	66	82	23
		2000	109	103	67	21	27
			109	115	123	38
			115	83	80	23
		3000	120	111	79	22	24
			100	84	76	25
			115	108	85	25
		4000	111	79	74	22	25
			102	62	74	24
			105	94	86	27
		5000	111	64	143	43	33
			110	65	91	28
			108	66	89	27
Positive Control	3-Methylcholanthrene	2.5	70	15	527	250	259*
			105	41	1008	320
			107	55	669	209

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination