Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01364-0005-0000-1
Chemical Name:	2-Naphthylamine
CASRN:	91-59-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	700736
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	104	103	37	37
			85	105	94	37
			82	112	97	39
			69	78	74	36
Test Chemical	2-Naphthylamine	32	69	100	91	44	47
			75	103	114	51	47
		64	70	87	122	58
		128	69	67	105	51	47
			98	69	130	44	47
		256	101	25	153	50	64*
			68	11	160	78	64*
		320

Positive Control	Ethyl Methane Sulfonate	500	32	23	957	992	1086*
			32	24	1088	1133
			30	25	1026	1134

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	93	108	37	27
			119#	94	85	24
			110	109	79	24
			113#	105	76.5	23
Test Chemical	2-Naphthylamine	158	91	43	219	81	66*
			98	48	148	51	66*
		186	83	35	209	84	70*
			90	37	153	56	70*
		219	108#	26	176	54	57*
			100	29	181	60	57*
		243	88	12	219	83	78*
			81	8	179	74	78*
		270	82	5	191	78	74*
			83	5	172	69	74*
		300

Positive Control	Ethyl Methane Sulfonate	500	65	38	1197	614	650*
			59	32	1236	696
			61	36	1173	639

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	104	70	32	35
			56	89	71	43
			85	107	77	30
			124r	149	103	28
Test Chemical	2-Naphthylamine	2.1	59	75	135	76	47
			73	84	41	19	47
		2.6	76	81	95	42	38
			69	78	72	35	38
		3.3	50	44	70	47	50
			59	51	95	54	50
		4.1	42	36	84	66	55
			56	40	75	44	55
		5.1	36	18	71	65	80*
			49	21	141	95	80*
		6.4	50	8	111	74	88*
			46	5	143	103	88*
		8

Positive Control	3-Methylcholanthrene	6	40	32	122	103	244*
			49	35	331	225
			38	29	455	404

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	85	135	65	78
			95	120	236	83
			69	97	152	74
			72	98	195	91
Test Chemical	2-Naphthylamine	2.36	78	79	223	96	88
			100	104	238	80	88
		4.72	90	31	360	133	147*
			81	30	395	162	147*
		5.25	87	24	493	188	178*
			75	21	374	167	178*
		5.83	79	14	508	214	232*
			64	14	480	249	232*
		6.48	69	8	657	319	287*
			53	6	403	254	287*
		7.2

Positive Control	3-Methylcholanthrene	5	55	41	758	457	512*
			44	25	831	625
			72	54	980	455

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination