Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01364-0006-0000-2
Chemical Name:	2-Naphthylamine
CASRN:	91-59-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	914276
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	89	29	12	15
			87	113	43	16
			89	110	38	14
			91	88	50	18
Test Chemical	2-Naphthylamine	3.9	75	78	51	23	19
			79	83	36	15	19
		7.8	79	93	42	18	21
			69	73	49	24	21
		15.6	80	81	61	26	25
			75	85	55	25	25
		31.3	86	89	72	28	24
			76	80	44	19	24
		62.5	91	91	23	8	14
			73	60	43	20	14
		125	65	22	58	30	23
			88	27	44	17	23
		250

Positive Control	Ethyl Methane Sulfonate	500	7r	2	433	2138	1826*
			8r	2	457	1865
			11	5	621	1826

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	105	65	26	25
			88	89	67	25
			88#	106	63	24
Test Chemical	2-Naphthylamine	50	88#	66	100	38	34
			70#	50	66	32
			74#	52	70	31
		100	71#	65	55.5	26	27
			58	61	61	35
			95#	107	57	20
		125	96	82	89	31	31
			72#	97	66	31	31
		150	80#	98	63	26	31
			73	113	67	31
			61	79	65	36
		200	69	117	60	29	27
			90#	120	69	26
			89	135	68	25
		250	37	11	78	71	72*
			46#	10	101	74	72*
		300


Positive Control	Ethyl Methane Sulfonate	250	36	35	746	684	693*
			30	29	647	723
			35#	33	711	671

Nonactivation Trial: 3 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	104#	113	66	21	13
			101	89	21	7
			88	98	28	11
Test Chemical	2-Naphthylamine	50	90	81	49	18	17
			100	118	53	18
			111#	98	47	14
		100	108	55	68	21	16
			101	82	32	11
			90	65	46	17
		150	102	47	67	22	26*
			100	29	93	31	26*
		200	92#	14	81	30	30*
			101	23	79	26
			85	29	89	35
Positive Control	Ethyl Methane Sulfonate	250	82	68	975	396	372*
			96	36	993	344
			80	62	896	376

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	107	101	56	17	16
			99	105	57	19
			95	83	38	13
			117	110	51	15
Test Chemical	2-Naphthylamine	50	85	48	49	19	23
			126r	130	55	15
			93	71	73	26
		100	92	43	36	13	20
			87	52	45	17
			96#	48	88.5	31
		150	79	28	26	11	18
			87	30	58	22
			81	29	53	22
		200	87	8	81	31	31*
			84	14	72	29
			92	14	90	32
Positive Control	Ethyl Methane Sulfonate	250	74	49	800	360	410*
			62	50	882	477
			65	47	766	394

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	114	125	62	18	19
			99	93	50	17
			97	88	68	23
			87	93	49	19
Test Chemical	2-Naphthylamine	100	117	59	62	18	18
			88	54	51	19
			97	73	48	17
		150	78	25	55	23	22
			103	34	60	19
			67	25	49	24
		200	54	7	62	38	35*
			76	17	71	31	35*
		225					36*
			52	4	54	35
			67	5	77	38
			76	12	79	35
		250					38*

			87	3	86	33
			72	3	91	42
		275



Positive Control	Ethyl Methane Sulfonate	250	52#	44	822	530	468*
			65	64	945	486
			78	62	903	388

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79#	78	33	14	15
			85	95	49	19
			94	106	50	18
			94	121	31	11
Test Chemical	2-Naphthylamine	0.125	60	76	39	22	17
			72#	69	22.5	10
			74	84	40	18
		0.25	67	62	30	15	15
			77	68	34	15	15
		0.5	81	78	37	15	19
			90#	68	37.5	14
			58	63	49	28
		1	76	44	57	25	29*
			73	44	60	27
			69	39	73	35
		2	62	16	82	44	34*
			76	15	47	21
			81	13	92	38
		4	76	4	281	124	116*
			64	6	208	109	116*
Positive Control	3-Methylcholanthrene	2.5	65	74	319	164	151*
			66	71	293	149
			63#	49	268.5	141

Induced S9 Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	108	48	18	15
			79	90	42	18
			109	116	37	11
			88	87	34	13
Test Chemical	2-Naphthylamine	0.25	91	108	19	7	12
			74#	88	39	18
			75	74	28	12
		0.5	67	61	20	10	8
			82	68	18	7
			69	57	17	8
		1	78	31	46	20	14
			63	33	13	7
			65	49	28	14
		2	48	9	38	27	26*
			105	20	40	13
			58	9	67	38
		3	70	3	66	31	22
			54	5	30	19
			72	9	35	16
		4


Positive Control	3-Methylcholanthrene	2.5	55	32	295	178	180*
			68#	40	304.5	150
			54	40	344	211

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102	101	126	41	34
Vehicle Control			101	99	81	27	34
Test Chemical	2-Naphthylamine	0.25	74#	83	63	28	20
			103#	84	55	18
			86	72	39	15
		0.5	103	85	85	28	23
			110	97	59	18	23
		1	90#	60	69	25	25
			80	35	69	29
			80	67	52	22
		2	66	13	89	45	46
			85	16	106	42
			86	25	131	51
		3	68	17	59	29	46
			75#	5	114	51
			79	7	97	41
			74	5	141	64
Positive Control	3-Methylcholanthrene	2.5	83	56	459	184	171*
			104	66	508	162
			93	67	465	166

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination