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Study Information
Study Attribute Value
CEBS Accession Number: 002-01368-0001-0000-1
Chemical Name: 2-Nitro-p-phenylenediamine
CASRN: 5307-14-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 186397_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 04/10/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Acetone 
0         
 50 1042 485 0.465 9.70 31.5 0.000
Test Chemical 2-Nitro-p-phenylenediamine 
1.7       
 50 1039 600 0.577 12.00 25.5 24.069 
5         
 50 1031 778 0.755 15.56 31.5 62.124 
16.7       
 50 1040 1120 1.077 22.40 31.5 131.372 
50         
 0 0 0 0.000 0.00 31.5 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1029 589 0.572 11.78 25.5 22.978
Mitomycin-C 
 0.01      
 5 103 173 1.680 34.60 25.5 260.857
Trend: 17.187        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 05/09/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Acetone 
0         
 25 519 282 0.543 11.28 35.0 0.000
Test Chemical 2-Nitro-p-phenylenediamine 
8.6       
 25 523 419 0.801 16.76 35.0 47.445 
16.8       
 25 523 505 0.966 20.20 35.0 77.708 
25         
 25 514 501 0.975 20.04 35.0 79.388 
35.2       
 0 0 0 0.000 0.00 35.0 N/A
Positive Control Mitomycin-C 
 0.001     
 25 508 283 0.557 11.32 25.5 2.528
Mitomycin-C 
 0.01      
 5 102 225 2.206 45.00 25.5 305.976
Trend: 8.286        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 04/10/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Acetone 
0         
 50 1027 425 0.414 8.50 25.5 0.000
Test Chemical 2-Nitro-p-phenylenediamine 
50         
 50 1045 494 0.473 9.88 25.5 14.233 
166.7       
 50 1029 622 0.604 12.44 25.5 46.068 
500         
 50 1028 992 0.965 19.84 25.5 133.185
Positive Control Cyclophosphamide 
 0.4       
 50 1028 695 0.676 13.90 25.5 63.370
Cyclophosphamide 
2         
 5 102 163 1.598 32.60 25.5 286.161
Trend: 16.268        
Probability: 0.000