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Study Information
Study Attribute Value
CEBS Accession Number: 002-01368-0002-0000-2
Chemical Name: 2-Nitro-p-phenylenediamine
CASRN: 5307-14-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 186397_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Positive 

Trial #:1   Activation: No Activation    Date: 06/12/1985    Harvest Time: 20.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Acetone 
0         
 100 6 0.060 4.0 1 0.010 1.0 5 0.050 3.0 0 0.000 0.0
Test Chemical: 2-Nitro-p-phenylenediamine 
300         
 100 7 0.070 6.0 2 0.020 2.0 5 0.050 4.0 0 0.000 0.0 
400         
 81 8 0.099 6.2 2 0.025 1.2 6 0.074 6.2 0 0.000 0.0 
500         
 76 10 0.132 9.2 0 0.000 0.0 10 0.132 9.2 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 100 22 0.220 15.0 9 0.090 8.0 13 0.130 9.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 11 0.440 32.0 3 0.120 8.0 8 0.320 24.0 0 0.000 0.0
Trend: 1.335 -0.674 1.893  
Probability: 0.091 0.750 0.029  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/24/1985    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Acetone 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: 2-Nitro-p-phenylenediamine 
300         
 100 11 0.110 8.0 3 0.030 3.0 8 0.080 6.0 0 0.000 0.0 
400         
 100 12 0.120 9.0 7 0.070 6.0 5 0.050 4.0 0 0.000 0.0 
500         
 100 25 0.250 17.0 13 0.130 11.0 12 0.120 8.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 100 10 0.100 8.0 1 0.010 1.0 9 0.090 8.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 14 0.560 36.0 5 0.200 16.0 9 0.360 24.0 0 0.000 0.0
Trend: 3.269 3.282 1.594  
Probability: 0.001 0.001 0.055  


Trial #:2   Activation: No Activation    Date: 05/21/1985    Harvest Time: 20.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 10 0.050 4.5 6 0.030 3.0 4 0.020 2.0 0 0.000 0.0
Acetone 
0         
 200 11 0.055 4.5 6 0.030 3.0 5 0.025 2.0 0 0.000 0.0
Test Chemical: 2-Nitro-p-phenylenediamine 
300         
 114 15 0.132 10.5 4 0.035 3.5 11 0.096 7.9 0 0.000 0.0 
400         
 200 20 0.100 9.0 6 0.030 2.5 14 0.070 6.5 0 0.000 0.0 
500         
 200 20 0.100 7.5 2 0.010 1.0 18 0.090 7.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 35 0.175 15.0 11 0.055 5.5 24 0.120 10.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 11 0.440 32.0 2 0.080 4.0 9 0.360 32.0 0 0.000 0.0
Trend: 1.092 -1.346 2.037  
Probability: 0.137 0.911 0.021  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 05/29/1985    Harvest Time: 12.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 12 0.060 5.0 6 0.030 2.5 6 0.030 3.0 0 0.000 0.0
Acetone 
0         
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0
Test Chemical: 2-Nitro-p-phenylenediamine 
300         
 200 23 0.115 8.5 15 0.075 6.5 8 0.040 2.5 0 0.000 0.0 
400         
 200 27 0.135 12.0 13 0.065 5.5 14 0.070 6.5 0 0.000 0.0 
500         
 200 28 0.140 11.5 11 0.055 5.5 17 0.085 7.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 29 0.145 13.0 8 0.040 4.0 21 0.105 9.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 24 0.960 64.0 10 0.400 40.0 14 0.560 44.0 0 0.000 0.0
Trend: 3.484 2.407 2.670  
Probability: 0.000 0.008 0.004