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Study Information
Study Attribute Value
CEBS Accession Number: 002-01368-0006-0000-6
Chemical Name: 2-Nitro-p-phenylenediamine
CASRN: 5307-14-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G10538
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 107 127 187 58 51
    92 130 113 41
    97 86 128 44
    89 57 159 60
Test Chemical 2-Nitro-p-phenylenediamine 
94         
 122r 151 214 58
    178r 173 334 63
    112 142 197 59
  
118         
 116 126 221 64 75
    127r 138 315 83
    106 121 272 86
  
148         
 103 106 345 112 111*
    75 77 286 127
    108 95 307 94
  
184         
 84 59 382 151 155*
    80 48 390 163
    83 39 373 149
  
230         
 88 39 406 153 139*
    75 36 314 139
    91 41 340 124
  
288         
 80 25 439 184 169*
    84 23 431 171
    81 20 371 153
Positive Control Ethyl Methane Sulfonate 
500         
 63 47 1413 754 797*
    57 46 1374 808
    53 44 1316 828

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 105 208 78 70
    81 91 181 74
    91 103 185 68
    95 102 176 62
Test Chemical 2-Nitro-p-phenylenediamine 
131         
 89 43 411 155 185*
    62 29 399 215
  
164         
 68 28 488 238 224*
    72 27 454 210
  
205         
 67 22 525 263 242*
    75 24 498 221
  
256         
 65 17 588 301 307*
    62 17 582 313
  
320         
 55 12 541 327 330*
    56 11 556 334
  
400         
 56 8 544 327 336*
    58 8 596 346
  
500         
 46 3 552 404 384*
    46 3 497 363
Positive Control Ethyl Methane Sulfonate 
500         
 36# 28 958 887 807*
    29 11 754 857
    53 42 1072 678

Non-Induced S9 Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 76 91 129 57 53
    76 99 112 49
    87 107 141 54
    87 103 137 52
Test Chemical 2-Nitro-p-phenylenediamine 
1.7       
 96 109 214 74 77
    69 78 163 79
  
2.1       
 81 96 167 68 59
    73 86 107 49
  
2.6       
 75 81 121 54 57
    86 101 152 59
  
3.3       
 72 78 124 58 59
    76 86 139 61
  
4.1       
 83 90 166 67 66
    81 88 157 65
  
5.1       
 82 101 191 78 63
    74# 77 107 48
  
6.4       
 73 83 140 64 67
    73 83 155 71
  
8         
 80 91 158 66 65
    78 89 150 64
Positive Control 3-Methylcholanthrene 
5         
 60 64 422 236 233*
    68 69 473 234
    65 65 448 229

Non-Induced S9 Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 105 177 68 63
    81 98 115 47
    72 91 157 73
    95 106 185 65
Test Chemical 2-Nitro-p-phenylenediamine 
4.2       
 96 103 220 76 76
    85 95 190 75
  
5.2       
 89 101 190 71 67
    113 135 210 62
  
6.6       
 91 102 159 58 73
    74 83 196 89
  
8.2       
 85 98 177 70 69
    87 102 179 69
  
10.2       
 83 91 137 55 66
    83 99 193 77
  
12.8       
 61 86 187 102 83
    74 94 145 65
  
16         
 74 78 175 79 81
    97 108 238 82
  
20         
 103 115 226 73 72
    85 92 180 71
  
25         
 114 124 323 95 89
    106 113 261 82
Positive Control 3-Methylcholanthrene 
5         
 94 105 783 278 308*
    72 78 693 323
    84 84 810 322

Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 63 92 231 122 109
    85 108 247 97
Test Chemical 2-Nitro-p-phenylenediamine 
1.03      
 61 18 179 99 88
    104 26 243 78
  
1.48      
 84 18 230 92 95
    90 17 262 97
  
2.11      
 81 19 223 92 92
    93 20 256 92
  
3.01      
 87 16 270 104 102
    85 12 252 99
  
4.3       
 55 10 244 147 136
    98 11 364 124
  
6.14      
 64 9 275 143 152
    64 7 310 161
  
7.68      
  
9.6       
   
Positive Control 3-Methylcholanthrene 
5         
 44 21 549 414 413*
    36 19 448 411

Induced S9 Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 65 98 129 66 62
    58 95 109 62
    67 112 133 66
    63# 95 102 54
Test Chemical 2-Nitro-p-phenylenediamine 
3.6       
 86 142 153 59 70
    81 162 168 69
    77 125 190 82
  
4.2       
 80 146 252 105 97*
    80 138 238 99
    73 141 190 86
  
4.9       
 72 123 240 112 80
    74# 159 133.5 61
    61 111 124 68
  
5.8       
 71# 125 217.5 102 90
    86 163 202 78
    74# 134 198 89
  
6.8       
 79 130 167 71 74
    78 123 161 69
    69 118 167 81
  
8         
 77# 121 120 52 65
    71# 119 136.5 64
    72# 105 171 79
Positive Control 3-Methylcholanthrene 
5         
 69# 93 451.5 218 266*
    70# 109 520.5 248
    55 74 545 330

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 104 254 98 102
    77 92 253 110
    88 104 216 82
    89 100 316 119
Test Chemical 2-Nitro-p-phenylenediamine 
2         
 91 106 275 101 100
    94 113 278 99
  
3.9       
 81 91 321 132
  
4.9       
 86 92 378 146 160*
    77 83 403 174
  
6.1       
 85 89 428 168 150
    105 98 417 133
  
7.7       
 78 66 451 193 173*
    91 79 417 152
  
9.6       
 72 32 523 243
  
12         
 45 7 748 552 469*
    54 10 625 386
Positive Control 3-Methylcholanthrene 
5         
 68 65 754 370 382*
    67 58 805 401
    71 72 801 374

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination