Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01368-0006-0000-6
Chemical Name:	2-Nitro-p-phenylenediamine
CASRN:	5307-14-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G10538
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	107	127	187	58	51
			92	130	113	41
			97	86	128	44
			89	57	159	60
Test Chemical	2-Nitro-p-phenylenediamine	94	122r	151	214	58
			178r	173	334	63
			112	142	197	59
		118	116	126	221	64	75
			127r	138	315	83
			106	121	272	86
		148	103	106	345	112	111*
			75	77	286	127
			108	95	307	94
		184	84	59	382	151	155*
			80	48	390	163
			83	39	373	149
		230	88	39	406	153	139*
			75	36	314	139
			91	41	340	124
		288	80	25	439	184	169*
			84	23	431	171
			81	20	371	153
Positive Control	Ethyl Methane Sulfonate	500	63	47	1413	754	797*
			57	46	1374	808
			53	44	1316	828

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	89	105	208	78	70
			81	91	181	74
			91	103	185	68
			95	102	176	62
Test Chemical	2-Nitro-p-phenylenediamine	131	89	43	411	155	185*
			62	29	399	215	185*
		164	68	28	488	238	224*
			72	27	454	210	224*
		205	67	22	525	263	242*
			75	24	498	221	242*
		256	65	17	588	301	307*
			62	17	582	313	307*
		320	55	12	541	327	330*
			56	11	556	334	330*
		400	56	8	544	327	336*
			58	8	596	346	336*
		500	46	3	552	404	384*
			46	3	497	363	384*
Positive Control	Ethyl Methane Sulfonate	500	36#	28	958	887	807*
			29	11	754	857
			53	42	1072	678

Non-Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	91	129	57	53
			76	99	112	49
			87	107	141	54
			87	103	137	52
Test Chemical	2-Nitro-p-phenylenediamine	1.7	96	109	214	74	77
			69	78	163	79	77
		2.1	81	96	167	68	59
			73	86	107	49	59
		2.6	75	81	121	54	57
			86	101	152	59	57
		3.3	72	78	124	58	59
			76	86	139	61	59
		4.1	83	90	166	67	66
			81	88	157	65	66
		5.1	82	101	191	78	63
			74#	77	107	48	63
		6.4	73	83	140	64	67
			73	83	155	71	67
		8	80	91	158	66	65
			78	89	150	64	65
Positive Control	3-Methylcholanthrene	5	60	64	422	236	233*
			68	69	473	234
			65	65	448	229

Non-Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	105	177	68	63
			81	98	115	47
			72	91	157	73
			95	106	185	65
Test Chemical	2-Nitro-p-phenylenediamine	4.2	96	103	220	76	76
			85	95	190	75	76
		5.2	89	101	190	71	67
			113	135	210	62	67
		6.6	91	102	159	58	73
			74	83	196	89	73
		8.2	85	98	177	70	69
			87	102	179	69	69
		10.2	83	91	137	55	66
			83	99	193	77	66
		12.8	61	86	187	102	83
			74	94	145	65	83
		16	74	78	175	79	81
			97	108	238	82	81
		20	103	115	226	73	72
			85	92	180	71	72
		25	114	124	323	95	89
			106	113	261	82	89
Positive Control	3-Methylcholanthrene	5	94	105	783	278	308*
			72	78	693	323
			84	84	810	322

Induced S9 Trial: 1 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	92	231	122	109
Vehicle Control			85	108	247	97	109
Test Chemical	2-Nitro-p-phenylenediamine	1.03	61	18	179	99	88
			104	26	243	78	88
		1.48	84	18	230	92	95
			90	17	262	97	95
		2.11	81	19	223	92	92
			93	20	256	92	92
		3.01	87	16	270	104	102
			85	12	252	99	102
		4.3	55	10	244	147	136
			98	11	364	124	136
		6.14	64	9	275	143	152
			64	7	310	161	152
		7.68
		9.6

Positive Control	3-Methylcholanthrene	5	44	21	549	414	413*
Positive Control			36	19	448	411	413*

Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	98	129	66	62
			58	95	109	62
			67	112	133	66
			63#	95	102	54
Test Chemical	2-Nitro-p-phenylenediamine	3.6	86	142	153	59	70
			81	162	168	69
			77	125	190	82
		4.2	80	146	252	105	97*
			80	138	238	99
			73	141	190	86
		4.9	72	123	240	112	80
			74#	159	133.5	61
			61	111	124	68
		5.8	71#	125	217.5	102	90
			86	163	202	78
			74#	134	198	89
		6.8	79	130	167	71	74
			78	123	161	69
			69	118	167	81
		8	77#	121	120	52	65
			71#	119	136.5	64
			72#	105	171	79
Positive Control	3-Methylcholanthrene	5	69#	93	451.5	218	266*
			70#	109	520.5	248
			55	74	545	330

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	104	254	98	102
			77	92	253	110
			88	104	216	82
			89	100	316	119
Test Chemical	2-Nitro-p-phenylenediamine	2	91	106	275	101	100
			94	113	278	99	100
		3.9	81	91	321	132
		4.9	86	92	378	146	160*
			77	83	403	174	160*
		6.1	85	89	428	168	150
			105	98	417	133	150
		7.7	78	66	451	193	173*
			91	79	417	152	173*
		9.6	72	32	523	243
		12	45	7	748	552	469*
			54	10	625	386	469*
Positive Control	3-Methylcholanthrene	5	68	65	754	370	382*
			67	58	805	401
			71	72	801	374

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination