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Study Information
Study Attribute Value
CEBS Accession Number: 002-01368-0007-0000-7
Chemical Name: 2-Nitro-p-phenylenediamine
CASRN: 5307-14-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G41276
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 104 102 59 19 25
    112 114 79 24
    71# 84 72 34
Test Chemical 2-Nitro-p-phenylenediamine 
31.3       
 85 61 208 82 90*
    76 65 225 99
  
62.5       
 111 61 262 79 91*
    100# 48 310 104
  
125         
 95 35 349 122 124*
    90 33 342 127
  
250         
 84 13 369 147 144*
    75 15 318 141
  
500         
 48 3 230 160 149*
    56 5 234 138
  
1000         
   
Positive Control Ethyl Methane Sulfonate 
500         
 52 28 752 482 519*
    49 29 813 557

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 94 81 31 27
    85 95 72 28
    79 115 63 27
    85 96 61 24
Test Chemical 2-Nitro-p-phenylenediamine 
31.3       
 76 81 125 55 49*
    91 89 117 43
  
62.5       
 91 62 207 76 78*
    72 80 171 79
  
125         
 108 29 330 102 106*
    64 40 212 110
  
250         
 92 16 269 97 108*
    76 16 273 119
  
375         
 71 8 250 117 123*
    73 15 283 130
Positive Control Ethyl Methane Sulfonate 
500         
 50 39 886 595 617*
    47 38 907 639

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 115 112 145 42 50
    106 103 170 53
    92 92 140 51
    90 93 141 52
Test Chemical 2-Nitro-p-phenylenediamine 
3.75      
 272r 148 174 21
    93 41 165 59
  
5         
 121r 59 208 57
    70 41 196 93
  
7.5       
 102 38 269 88 83*
    98 36 230 78
  
10         
 81 28 194 80 87*
    81 27 229 95
  
15         
 78 15 198 84 86*
    98 24 257 88
Positive Control 3-Methylcholanthrene 
5         
 40 12 491 406 407*
    35 17 422 408

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 101 33 13 18
    105 103 61 19
    80 95 44 18
    98 101 61 21
Test Chemical 2-Nitro-p-phenylenediamine 
2.5       
 95 59 68 24 23
    95 60 61 21
    126r 66 108 29
  
5         
 89 49 87 33 27*
    98 57 83 28
    112 57 71 21
  
10         
 108 43 89 28 25
    125r 58 88 24
    100 46 69 23
  
15         
 103# 39 70.5 23 28*
    116 47 84 24
    102 44 117 38
  
20         
 111 23 134 40 37*
    107 33 101 32
    83 26 93 38
  
25         
 99 20 139 47 43*
    83 25 102 41
    90 26 114 42
Positive Control 3-Methylcholanthrene 
5         
 59 16 433 245 236*
    52 20 354 225
    58 28 416 238

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 66 73 60 31 31
    86 114 86 33
    68 93 62 30
    81 119 71 29
Test Chemical 2-Nitro-p-phenylenediamine 
5         
 77 61 99 43 34
    88 66 69 26
    81 67 83 34
  
10         
 74 30 96 43 39
    70 40 76 36
    66 30 75 38
  
15         
 43 13 73 57 45
    90 25 119 44
    95 22 100 35
  
20         
 58 9 88 51 58*
    65 9 123 63
    45 11 81 60
  
25         
 47 5 126 89 80*
    61 8 101 55
    36 5 105 97
  
30         
 56 6 81 49 57*
    72 11 122 56
    54 5 110 67
  
35         
   
   
Positive Control 3-Methylcholanthrene 
5         
 47 19 530 377 341*
    36 21 311 291
    36 21 386 354

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination