Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01368-0007-0000-7
Chemical Name:	2-Nitro-p-phenylenediamine
CASRN:	5307-14-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G41276
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	104	102	59	19	25
			112	114	79	24
			71#	84	72	34
Test Chemical	2-Nitro-p-phenylenediamine	31.3	85	61	208	82	90*
			76	65	225	99	90*
		62.5	111	61	262	79	91*
			100#	48	310	104	91*
		125	95	35	349	122	124*
			90	33	342	127	124*
		250	84	13	369	147	144*
			75	15	318	141	144*
		500	48	3	230	160	149*
			56	5	234	138	149*
		1000

Positive Control	Ethyl Methane Sulfonate	500	52	28	752	482	519*
Positive Control			49	29	813	557	519*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	94	81	31	27
			85	95	72	28
			79	115	63	27
			85	96	61	24
Test Chemical	2-Nitro-p-phenylenediamine	31.3	76	81	125	55	49*
			91	89	117	43	49*
		62.5	91	62	207	76	78*
			72	80	171	79	78*
		125	108	29	330	102	106*
			64	40	212	110	106*
		250	92	16	269	97	108*
			76	16	273	119	108*
		375	71	8	250	117	123*
			73	15	283	130	123*
Positive Control	Ethyl Methane Sulfonate	500	50	39	886	595	617*
Positive Control			47	38	907	639	617*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	115	112	145	42	50
			106	103	170	53
			92	92	140	51
			90	93	141	52
Test Chemical	2-Nitro-p-phenylenediamine	3.75	272r	148	174	21
			93	41	165	59
		5	121r	59	208	57
			70	41	196	93
		7.5	102	38	269	88	83*
			98	36	230	78	83*
		10	81	28	194	80	87*
			81	27	229	95	87*
		15	78	15	198	84	86*
			98	24	257	88	86*
Positive Control	3-Methylcholanthrene	5	40	12	491	406	407*
Positive Control			35	17	422	408	407*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	101	33	13	18
			105	103	61	19
			80	95	44	18
			98	101	61	21
Test Chemical	2-Nitro-p-phenylenediamine	2.5	95	59	68	24	23
			95	60	61	21
			126r	66	108	29
		5	89	49	87	33	27*
			98	57	83	28
			112	57	71	21
		10	108	43	89	28	25
			125r	58	88	24
			100	46	69	23
		15	103#	39	70.5	23	28*
			116	47	84	24
			102	44	117	38
		20	111	23	134	40	37*
			107	33	101	32
			83	26	93	38
		25	99	20	139	47	43*
			83	25	102	41
			90	26	114	42
Positive Control	3-Methylcholanthrene	5	59	16	433	245	236*
			52	20	354	225
			58	28	416	238

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	73	60	31	31
			86	114	86	33
			68	93	62	30
			81	119	71	29
Test Chemical	2-Nitro-p-phenylenediamine	5	77	61	99	43	34
			88	66	69	26
			81	67	83	34
		10	74	30	96	43	39
			70	40	76	36
			66	30	75	38
		15	43	13	73	57	45
			90	25	119	44
			95	22	100	35
		20	58	9	88	51	58*
			65	9	123	63
			45	11	81	60
		25	47	5	126	89	80*
			61	8	101	55
			36	5	105	97
		30	56	6	81	49	57*
			72	11	122	56
			54	5	110	67
		35


Positive Control	3-Methylcholanthrene	5	47	19	530	377	341*
			36	21	311	291
			36	21	386	354

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination