| Study Attribute | Value | 
|---|---|
| CEBS Accession Number: | 002-01385-0003-0000-2 | 
| Chemical Name: | 3,3',5,5'-Tetramethylbenzidine | 
| CASRN: | 54827-17-7 | 
| NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity | 
| NTP Study ID: | 831590 | 
| Study Result: | Positive | 
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Solvent | 0 | 99 | 110 | 139 | 47 | 51 | 
| 86 | 97 | 121 | 47 | ||||
| 95 | 102 | 122 | 43 | ||||
| 84 | 91 | 164 | 65 | ||||
| Test Chemical | 3,3',5,5'-Tetramethylbenzidine | 15.4 | 91 | 82 | 242 | 89 | 79 | 
| 78# | 62 | 160 | 69 | ||||
| 30.7 | 99 | 98 | 250 | 84 | 79* | ||
| 73 | 74 | 163 | 74 | ||||
| 51.2 | 70 | 30 | 278 | 133 | 133* | ||
| 61 | 14 | 243 | 133 | ||||
| 100 | 58 | 18 | 504 | 289 | 208* | ||
| 72 | 33 | 275 | 128 | ||||
| 125 | 67 | 19 | 438 | 217 | 181* | ||
| 74 | 27 | 320 | 144 | ||||
| Positive Control | Ethyl Methane Sulfonate | 500 | 42 | 36 | 990 | 783 | 617* | 
| 46 | 39 | 1125 | 821 | ||||
| 98 | 120 | 726 | 248 | ||||
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Dimethyl Sulfoxide | 0 | 58 | 89 | 75 | 43 | 49 | 
| 55 | 74 | 111 | 68 | ||||
| 72# | 137 | 93 | 43 | ||||
| 58 | 101 | 73 | 42 | ||||
| Test Chemical | 3,3',5,5'-Tetramethylbenzidine | 42 | 45 | 12 | 96 | 72 | 62 | 
| 34 | 10 | 52 | 51 | ||||
| 53 | 15 | 5 | 40 | 88 | 79 | ||
| 30 | 6 | 63 | 69 | ||||
| 66 | |||||||
| 25 | 3 | 69 | 91 | ||||
| 82 | |||||||
| Positive Control | Ethyl Methane Sulfonate | 500 | 40 | 50 | 955 | 803 | 843* | 
| 30 | 29 | 813 | 908 | ||||
| 37 | 41 | 903 | 817 | ||||
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Dimethyl Sulfoxide | 0 | 85 | 106 | 54 | 21 | 24 | 
| 86 | 81 | 74 | 29 | ||||
| 83 | 116 | 41 | 17 | ||||
| 84 | 97 | 75 | 30 | ||||
| Test Chemical | 3,3',5,5'-Tetramethylbenzidine | 26 | 64 | 93 | 82 | 43 | 37* | 
| 89 | 111 | 73 | 27 | ||||
| 74 | 100 | 91 | 41 | ||||
| 33 | 71 | 92 | 68 | 32 | 23 | ||
| 120 | 163 | 53 | 15 | ||||
| 124r | 161 | 116 | 31 | ||||
| 41 | 81 | 98 | 74 | 30 | 32 | ||
| 76 | 89 | 102 | 45 | ||||
| 85 | 94 | 54 | 21 | ||||
| 51 | 73 | 53 | 101 | 46 | 59* | ||
| 66 | 28 | 151 | 76 | ||||
| 73 | 60 | 118 | 54 | ||||
| 64 | |||||||
| Positive Control | Ethyl Methane Sulfonate | 500 | 59 | 50 | 1163 | 655 | 702* | 
| 49 | 45 | 1110 | 750 | ||||
| 64 | 59 | 1354 | 702 | ||||
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Dimethyl Sulfoxide | 0 | 75 | 111 | 47 | 21 | 26 | 
| 53 | 77 | 46 | 29 | ||||
| 73 | 112 | 61 | 28 | ||||
| Test Chemical | 3,3',5,5'-Tetramethylbenzidine | 41 | 78 | 138 | 55 | 24 | 21 | 
| 83 | 146 | 48 | 19 | ||||
| 51 | 89 | 142 | 69 | 26 | 27 | ||
| 60 | 103 | 52 | 29 | ||||
| 64 | 72 | 118 | 22 | 10 | 17 | ||
| 68 | 116 | 47 | 23 | ||||
| 80 | 68 | 114 | 47 | 23 | 25 | ||
| 69 | 117 | 56 | 27 | ||||
| 100 | 72 | 118 | 51 | 24 | 24 | ||
| 70 | 106 | 53 | 25 | ||||
| 125 | 60 | 84 | 46 | 26 | 30 | ||
| 61 | 83 | 64 | 35 | ||||
| Positive Control | 3-Methylcholanthrene | 5 | 43 | 54 | 217 | 168 | 162* | 
| 47 | 56 | 247 | 176 | ||||
| 54 | 60 | 230 | 143 | ||||
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Dimethyl Sulfoxide | 0 | 100 | 116 | 331 | 110 | 102 | 
| 112 | 99 | 399 | 119 | ||||
| 104 | 85 | 236 | 76 | ||||
| Test Chemical | 3,3',5,5'-Tetramethylbenzidine | 52 | 85# | 100 | 225 | 88 | 83 | 
| 81# | 135 | 163 | 67 | ||||
| 55 | 74 | 155 | 94 | ||||
| 66 | 80 | 79 | 276 | 116 | 85 | ||
| 80 | 127 | 150 | 62 | ||||
| 78 | 105 | 179 | 76 | ||||
| 82 | 82 | 78 | 212 | 86 | 85 | ||
| 93 | 99 | 213 | 77 | ||||
| 88 | 81 | 240 | 91 | ||||
| 102 | 86 | 81 | 204 | 79 | 74 | ||
| 82 | 74 | 172 | 70 | ||||
| 91 | 95 | 199 | 73 | ||||
| 128 | 105 | 90 | 154 | 49 | 54 | ||
| 118 | 106 | 209 | 59 | ||||
| 97 | 87 | 161 | 55 | ||||
| Positive Control | 3-Methylcholanthrene | 5 | 61 | 33 | 814 | 445 | 460* | 
| 77 | 38 | 1002 | 437 | ||||
| 60 | 32 | 900 | 497 | ||||
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination
 
                        