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Study Information
Study Attribute Value
CEBS Accession Number: 002-01389-0003-0000-6
Chemical Name: 3,3'-Dimethoxybenzidine
CASRN: 119-90-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 014473_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Weakly Positive 
No Activation  Positive 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 09/09/1980    Trial Call: Weakly Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1048 424 0.405 8.48 26.0 0.000
Test Chemical 3,3'-Dimethoxybenzidine
 0.005     
50 1050 432 0.411 8.64 26.0 1.693
 0.05      
50 1046 394 0.377 7.88 26.0 -6.898
 0.5       
50 1048 415 0.396 8.30 26.0 -2.123
5         
50 43 474 11.023 9.48 26.0 2624.616
50         
50 1043 727 0.697 14.54 26.0 72.284
Positive Control Triethylenemelamine
 0.025     
50 1051 2429 2.311 48.58 26.0 471.242
Trend: 17.753        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 02/04/1981    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1050 422 0.402 8.44 26.0 0.000
Test Chemical 3,3'-Dimethoxybenzidine
 0.5       
50 1049 425 0.405 8.50 26.0 0.807
1.6       
50 1049 456 0.435 9.12 26.0 8.160
5         
50 1049 515 0.491 10.30 26.0 22.154
16         
50 1048 598 0.571 11.96 26.0 41.977
50         
50 1047 825 0.788 16.50 26.0 96.058
Positive Control Triethylenemelamine
 0.025     
50 1050 2661 2.534 53.22 26.0 530.569
Trend: 13.373        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/09/1980    Trial Call: Negative   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1052 368 0.350 7.36 26.0 0.000
Test Chemical 3,3'-Dimethoxybenzidine
 0.005     
50 1053 443 0.421 8.86 26.0 20.266
 0.05      
50 1047 406 0.388 8.12 26.0 10.853
 0.5       
50 1048 405 0.386 8.10 26.0 10.474
5         
50 1050 419 0.399 8.38 26.0 14.076
50         
50 1049 433 0.413 8.66 26.0 18.000
Positive Control Cyclophosphamide
1.5       
50 1049 1706 1.626 34.12 26.0 364.913
Trend: 1.536        
Probability: 0.062        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 02/04/1981    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1049 450 0.429 9.00 26.0 0.000
Test Chemical 3,3'-Dimethoxybenzidine
50         
50 1048 418 0.399 8.36 26.0 -7.022
160         
50 1048 461 0.440 9.22 26.0 2.542
500         
50 1049 465 0.443 9.30 26.0 3.333
1600         
50 1048 546 0.521 10.92 26.0 21.449
5000         
50 1049 719 0.685 14.38 26.0 59.778
Positive Control Cyclophosphamide
1.5       
50 1050 1957 1.864 39.14 26.0 334.475
Trend: 9.163        
Probability: 0.000