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Study Information
Study Attribute Value
CEBS Accession Number: 002-01389-0004-0000-7
Chemical Name: 3,3'-Dimethoxybenzidine
CASRN: 119-90-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 014473_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 02/04/1981    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3,3'-Dimethoxybenzidine 
50         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
160         
 100 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 3 0.030 2.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
1600         
 100 6 0.060 6.0 1 0.010 1.0 5 0.050 5.0 0 0.000 0.0 
5000         
 100 7 0.070 6.0 3 0.030 2.0 4 0.040 4.0 0 0.000 0.0
Positive Control: Triethylenemelamine 
25         
 100 35 0.350 26.0 14 0.140 13.0 21 0.210 17.0 0 0.000 0.0
Trend: 2.148 0.690 2.216  
Probability: 0.016 0.245 0.013  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/04/1981    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 6 0.060 6.0 0 0.000 0.0 6 0.060 6.0 0 0.000 0.0
Test Chemical: 3,3'-Dimethoxybenzidine 
50         
 100 5 0.050 5.0 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 
160         
 100 5 0.050 5.0 0 0.000 0.0 5 0.050 5.0 0 0.000 0.0 
500         
 100 12 0.120 11.0 4 0.040 4.0 8 0.080 7.0 0 0.000 0.0 
1600         
 100 7 0.070 6.0 3 0.030 2.0 4 0.040 4.0 0 0.000 0.0 
5000         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
25         
 100 14 0.140 10.0 4 0.040 4.0 10 0.100 7.0 0 0.000 0.0
Trend: -0.561 0.096 -0.704  
Probability: 0.713 0.462 0.759  


Trial #:2   Activation: No Activation    Date: 09/09/1980    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3,3'-Dimethoxybenzidine 
 0.005     
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
 0.05      
 100 4 0.040 3.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
 0.5       
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
5         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
50         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Triethylenemelamine 
 0.25      
 100 46 0.460 30.0 21 0.210 17.0 25 0.250 20.0 0 0.000 0.0
Trend: 1.724 0.588 1.494  
Probability: 0.042 0.278 0.068  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 09/09/1980    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3,3'-Dimethoxybenzidine 
 0.005     
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
 0.05      
 100 4 0.040 3.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
 0.5       
 100 7 0.070 5.0 0 0.000 0.0 7 0.070 5.0 0 0.000 0.0 
5         
 100 6 0.060 6.0 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0 
50         
 100 6 0.060 5.0 1 0.010 1.0 5 0.050 4.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
25         
 100 90 0.900 55.0 40 0.400 35.0 47 0.470 33.0 3 0.030 3.0
Trend: 2.289 1.441 1.657  
Probability: 0.011 0.075 0.049