Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01389-0013-0000-7 |
Chemical Name: | 3,3'-Dimethoxybenzidine dihydrochloride |
CASRN: | 20325-40-0 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 640872 |
Study Result: | Positive |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Ethanol | 0 | 45r | 101 | 15 | 11 | 29 |
42r | 96 | 43 | 34 | ||||
50 | 107 | 44 | 29 | ||||
52 | 93 | 45 | 29 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 26 | 27 | 37 | 27 | 33 | 38 |
42 | 70 | 63 | 51 | ||||
38 | 57 | 35 | 31 | ||||
33 | 27 | 44 | 26 | 32 | 44 | ||
39 | 56 | 59 | 50 | ||||
36 | 45 | 55 | 51 | ||||
41 | 111# | 136 | 48 | 14 | 45 | ||
46 | 49 | 70 | 51 | ||||
39 | 44 | 79 | 68 | ||||
51 | 41 | 62 | 62 | 51 | 60* | ||
35 | 14 | 74 | 70 | ||||
64 | 18 | 2 | 74 | 138 | 78* | ||
36 | 42 | 55 | 52 | ||||
34 | 25 | 46 | 45 | ||||
Positive Control | Ethyl Methane Sulfonate | 500 | 26 | 35 | 658 | 849 | 787* |
28 | 38 | 643 | 756 | ||||
28 | 43 | 639 | 756 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Ethanol | 0 | 61 | 98 | 103 | 56 | 58 |
77 | 103 | 121 | 53 | ||||
69 | 99 | 143 | 69 | ||||
70 | 100 | 110 | 52 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 26 | 70 | 80 | 122 | 58 | 57 |
69 | 82 | 118 | 57 | ||||
33 | 80 | 71 | 204 | 85 | 97* | ||
54 | 49 | 174 | 108 | ||||
41 | 60 | 63 | 186 | 103 | 114* | ||
43 | 39 | 159 | 124 | ||||
51 | 51 | 22 | 267 | 174 | 194* | ||
50 | 13 | 321 | 215 | ||||
64 | 39 | 4 | 221 | 191 | 217* | ||
30 | 2 | 221 | 243 | ||||
80 | |||||||
Positive Control | Ethyl Methane Sulfonate | 500 | 41 | 44 | 829 | 674 | 667* |
45 | 42 | 882 | 658 | ||||
41 | 43 | 824 | 670 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Ethanol | 0 | 61 | 96 | 80 | 43 | 45 |
57 | 98 | 76 | 45 | ||||
65 | 105 | 91 | 47 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 210 | 60# | 100 | 88.5 | 49 | 61 |
73 | 126 | 159 | 73 | ||||
262 | 71 | 107 | 91 | 43 | 75* | ||
63 | 85 | 203 | 108 | ||||
328 | 58 | 51 | 159 | 91 | 91* | ||
49 | 70 | 133 | 91 | ||||
Positive Control | 3-Methylcholanthrene | 5 | 57 | 72 | 387 | 226 | 221* |
54 | 70 | 348 | 216 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Ethanol | 0 | 99 | 114 | 110 | 37 | 44 |
88 | 98 | 136 | 52 | ||||
114 | 111 | 128 | 37 | ||||
80 | 78 | 117 | 49 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 211 | 87 | 75 | 225 | 87 | 69* |
88 | 105 | 151 | 57 | ||||
98 | 117 | 186 | 63 | ||||
354 | 96 | 96 | 217 | 76 | 71* | ||
80 | 81 | 192 | 80 | ||||
97 | 92 | 170 | 58 | ||||
394 | 93 | 77 | 205 | 74 | 68* | ||
88 | 62 | 189 | 72 | ||||
103 | 100 | 184 | 60 | ||||
437 | 84 | 62 | 275 | 109 | 93* | ||
88 | 78 | 228 | 87 | ||||
80 | 78 | 204 | 85 | ||||
486 | 74r | 64 | 196 | 88 | |||
540 | 96 | 76 | 233 | 81 | 73* | ||
103 | 96 | 204 | 66 | ||||
90 | 81 | 192 | 71 | ||||
600 | 87 | 89 | 191 | 73 | 83* | ||
97 | 90 | 208 | 72 | ||||
89 | 73 | 276 | 104 | ||||
Positive Control | 3-Methylcholanthrene | 5 | 69 | 41 | 787 | 378 | 429* |
60 | 34 | 630 | 353 | ||||
52 | 27 | 871 | 557 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination