Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01389-0014-0000-8 |
Chemical Name: | 3,3'-Dimethoxybenzidine dihydrochloride |
CASRN: | 20325-40-0 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 948661 |
Study Result: | Positive |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Solvent | 0 | 97 | 93 | 60 | 21 | 25 |
93 | 105 | 83 | 30 | ||||
80 | 97 | 65 | 27 | ||||
91 | 104 | 64 | 24 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 40 | 75 | 56 | 49 | 22 | 20 |
57 | 84 | 26 | 15 | ||||
75 | 62 | 55 | 24 | ||||
50 | 86 | 58 | 48 | 19 | 19 | ||
53# | 49 | 27 | 17 | ||||
90 | 76 | 55 | 20 | ||||
60 | 79 | 56 | 48 | 20 | 19 | ||
70# | 49 | 30 | 14 | ||||
62 | 54 | 41 | 22 | ||||
70 | 62 | 26 | 47 | 25 | 29 | ||
63 | 25 | 63 | 33 | ||||
80 | 56 | 13 | 54 | 32 | 35 | ||
52# | 10 | 66 | 42 | ||||
48 | 10 | 45 | 32 | ||||
90 | 51 | 6 | 71 | 46 | 43* | ||
63 | 10 | 56 | 29 | ||||
55 | 8 | 86 | 52 | ||||
Positive Control | Ethyl Methane Sulfonate | 500 | 27 | 11 | 660 | 810 | 953* |
34 | 18 | 908 | 886 | ||||
20 | 9 | 680 | 1162 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Solvent | 0 | 84 | 109 | 67 | 27 | 27 |
94 | 91 | 77 | 27 | ||||
165r | 178 | 54 | 11 | ||||
125r | 150 | 51 | 14 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 40 | 61 | 58 | 42 | 23 | 28 |
90 | 54 | 91 | 34 | ||||
83 | 90 | 69 | 28 | ||||
50 | 87 | 59 | 47 | 18 | 29 | ||
51 | 32 | 65 | 43 | ||||
70 | 60 | 57 | 27 | ||||
60 | 96 | 32 | 120 | 42 | 61* | ||
73 | 28 | 119 | 55 | ||||
42 | 7 | 108 | 85 | ||||
70 | 59 | 10 | 131 | 74 | 74* | ||
59 | 16 | 116 | 66 | ||||
70 | 10 | 176 | 84 | ||||
80 | 50# | 13 | 92 | 61 | 72* | ||
74 | 12 | 139 | 63 | ||||
53 | 7 | 146 | 92 | ||||
Positive Control | Ethyl Methane Sulfonate | 250 | 79 | 69 | 743 | 314 | 283* |
109 | 77 | 761 | 233 | ||||
89# | 70 | 807 | 303 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Solvent | 0 | 9r | 9 | 37.5 | 134 | 13 |
85 | 107 | 25 | 10 | ||||
71 | 93 | 33 | 16 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 40 | 82 | 48 | 35 | 14 | 14 |
91 | 60 | 30 | 11 | ||||
82 | 54 | 42 | 17 | ||||
50 | 70 | 29 | 38 | 18 | 17 | ||
72# | 34 | 36 | 17 | ||||
68 | 48 | 31 | 15 | ||||
60 | 61 | 20 | 48 | 26 | 22 | ||
76 | 26 | 40 | 17 | ||||
70 | 58 | 3 | 136 | 78 | 59* | ||
78# | 8 | 94.5 | 40 | ||||
80 | |||||||
68 | 5 | 101 | 50 | ||||
90 | |||||||
Positive Control | Ethyl Methane Sulfonate | 250 | 60 | 61 | 617 | 346 | 674* |
20 | 16 | 781 | 1302 | ||||
55 | 47 | 622 | 375 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Water | 0 | 103 | 90 | 53 | 17 | 22 |
94 | 98 | 70 | 25 | ||||
96 | 103 | 72 | 25 | ||||
88 | 110 | 60 | 23 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 40 | 83 | 70 | 62 | 25 | 26 |
100 | 74 | 45 | 15 | ||||
53 | 47 | 62 | 39 | ||||
50 | 97 | 59 | 75 | 26 | 22 | ||
85 | 64 | 41 | 16 | ||||
92 | 77 | 65 | 24 | ||||
60 | 75 | 43 | 75 | 33 | 37* | ||
77 | 26 | 104 | 45 | ||||
94 | 44 | 89 | 32 | ||||
70 | 93 | 45 | 94 | 34 | 30 | ||
81 | 38 | 72 | 30 | ||||
70 | 45 | 54 | 26 | ||||
80 | 52 | 7 | 96 | 62 | 61* | ||
49 | 5 | 99 | 67 | ||||
71 | 9 | 116 | 54 | ||||
90 | 37 | 2 | 85 | 76 | 66* | ||
43 | 4 | 101 | 79 | ||||
60 | 14 | 76 | 42 | ||||
Positive Control | Ethyl Methane Sulfonate | 250 | 64 | 55 | 842 | 439 | 380* |
80 | 57 | 808 | 339 | ||||
80 | 64 | 868 | 363 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Solvent | 0 | 82 | 97 | 95 | 39 | 36 |
86 | 105 | 76 | 30 | ||||
92 | 88 | 115 | 42 | ||||
102 | 110 | 103 | 34 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 4.7 | 98 | 103 | 120 | 41 | 36 |
87 | 86 | 80 | 31 | ||||
9.4 | 85 | 95 | 108 | 42 | 39 | ||
87 | 85 | 93 | 36 | ||||
18.8 | 63 | 77 | 84 | 44 | 48 | ||
86 | 81 | 133 | 52 | ||||
37.5 | 91 | 90 | 126 | 46 | 46 | ||
90 | 103 | 122 | 45 | ||||
75 | 65 | 73 | 130 | 66 | 63* | ||
87 | 105 | 157 | 60 | ||||
150 | 118 | 90 | 149 | 42 | 64* | ||
79 | 75 | 203 | 85 | ||||
Positive Control | 3-Methylcholanthrene | 5 | 24 | 2 | 256 | 353 | 338* |
30 | 3 | 277 | 304 | ||||
25 | 2 | 267 | 356 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Solvent | 0 | 89 | 113 | 87 | 33 | 29 |
70 | 114 | 69 | 33 | ||||
87 | 91 | 51 | 19 | ||||
70 | 82 | 62 | 30 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 100 | 65 | 74 | 102 | 52 | 55* |
62 | 61 | 102 | 55 | ||||
70 | 71 | 121 | 58 | ||||
125 | 52 | 83 | 71 | 46 | 53* | ||
57 | 76 | 88 | 52 | ||||
55 | 70 | 101 | 62 | ||||
150 | 67 | 66 | 104 | 52 | 49* | ||
68 | 62 | 126 | 62 | ||||
54 | 62 | 53 | 33 | ||||
200 | 63 | 56 | 90 | 48 | 45* | ||
89 | 32 | 118 | 44 | ||||
62 | 58 | 79 | 42 | ||||
250 | 76 | 64 | 143 | 63 | 62* | ||
60 | 70 | 100 | 56 | ||||
54 | 48 | 107 | 66 | ||||
Positive Control | 3-Methylcholanthrene | 5 | 23 | 5 | 167 | 244 | 272* |
24 | 4 | 203 | 282 | ||||
26 | 4 | 224 | 289 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Solvent | 0 | 93 | 107 | 57 | 20 | 17 |
77 | 130 | 38 | 16 | ||||
76 | 89 | 25 | 11 | ||||
72 | 73 | 41 | 19 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 50 | 72 | 67 | 79 | 37 | 33* |
78 | 80 | 80 | 34 | ||||
74# | 96 | 60 | 27 | ||||
100 | 69# | 59 | 94.5 | 46 | 37* | ||
82 | 50 | 72 | 29 | ||||
63# | 66 | 66 | 35 | ||||
150 | 87 | 95 | 92 | 35 | 37* | ||
93 | 70 | 120 | 43 | ||||
63 | 72 | 62 | 33 | ||||
200 | 61 | 57 | 84 | 46 | 36* | ||
50 | 64 | 56 | 37 | ||||
86 | 90 | 67 | 26 | ||||
250 | 66 | 80 | 60 | 30 | 37* | ||
65 | 74 | 73 | 38 | ||||
56 | 45 | 71 | 42 | ||||
300 | 52 | 29 | 57 | 36 | 32* | ||
60 | 63 | 46 | 25 | ||||
64 | 44 | 69 | 36 | ||||
60 | 47 | 58 | 32 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 54 | 44 | 299 | 183 | 188* |
69 | 41 | 405 | 196 | ||||
61# | 52 | 342 | 186 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Water | 0 | 94 | 83 | 99 | 35 | 34 |
133r | 113 | 114 | 29 | ||||
108 | 112 | 101 | 31 | ||||
110 | 105 | 115 | 35 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 50 | 98 | 94 | 164 | 56 | 60* |
96 | 89 | 168 | 58 | ||||
103 | 73 | 204 | 66 | ||||
100 | 130r | 97 | 203 | 52 | 59* | ||
113# | 100 | 193.5 | 57 | ||||
96 | 71 | 176 | 61 | ||||
200 | 80 | 42 | 171 | 71 | |||
250 | 128r | 98 | 187 | 49 | 62* | ||
109# | 77 | 234 | 71 | ||||
118 | 82 | 189 | 53 | ||||
300 | 85# | 65 | 217.5 | 86 | 82* | ||
98 | 57 | 235 | 80 | ||||
104# | 59 | 253.5 | 81 | ||||
400 | 132* | ||||||
72 | 10 | 365 | 169 | ||||
82 | 13 | 232 | 95 | ||||
500 | |||||||
Positive Control | 3-Methylcholanthrene | 2.5 | 85 | 17 | 628 | 247 | 440* |
34 | 4 | 398 | 394 | ||||
27# | 3 | 546 | 678 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Water | 0 | 87# | 96 | 75 | 29 | 29 |
110 | 124 | 63 | 19 | ||||
97 | 93 | 91 | 31 | ||||
78# | 87 | 85.5 | 37 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 50 | 90 | 87 | 183 | 68 | 62* |
97 | 90 | 150 | 51 | ||||
95 | 92 | 186 | 66 | ||||
100 | 84 | 97 | 149 | 59 | 66* | ||
90 | 93 | 184 | 69 | ||||
74 | 76 | 155 | 70 | ||||
200 | 102 | 103 | 176 | 58 | 67* | ||
90 | 71 | 204 | 76 | ||||
120r | 104 | 169 | 47 | ||||
250 | 101 | 79 | 162 | 53 | 58* | ||
96 | 75 | 162 | 56 | ||||
92 | 63 | 181 | 66 | ||||
300 | 90 | 48 | 149 | 55 | 64* | ||
63 | 46 | 122 | 65 | ||||
64 | 57 | 138 | 72 | ||||
83 | 58 | 154 | 62 | ||||
400 | 102 | 30 | 237 | 78 | 100* | ||
52 | 12 | 195 | 126 | ||||
74 | 12 | 253 | 114 | ||||
72 | 18 | 176 | 82 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 52 | 34 | 388 | 250 | 257* |
59 | 33 | 519 | 293 | ||||
64# | 40 | 435 | 228 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Water | 0 | 72# | 71 | 52 | 24 | 20 |
87 | 129 | 42 | 16 | ||||
87 | 96 | 55 | 21 | ||||
85 | 105 | 46 | 18 | ||||
Test Chemical | 3,3'-Dimethoxybenzidine dihydrochloride | 50 | 57# | 64 | 63 | 37 | 35* |
60 | 74 | 67 | 37 | ||||
43 | 43 | 40 | 31 | ||||
100 | 69 | 50 | 110 | 54 | 48* | ||
53 | 60 | 83 | 52 | ||||
65 | 64 | 76 | 39 | ||||
200 | 45 | 50 | 69 | 51 | 52* | ||
51# | 45 | 67 | 44 | ||||
43 | 45 | 78 | 60 | ||||
250 | 67 | 61 | 81 | 40 | 38* | ||
74 | 72 | 95 | 43 | ||||
90 | 70 | 81 | 30 | ||||
300 | 57 | 58 | 73 | 43 | 46* | ||
56 | 53 | 71 | 42 | ||||
75# | 57 | 112.5 | 50 | ||||
57 | 60 | 84 | 49 | ||||
400 | 72 | 55 | 127 | 59 | 49* | ||
74 | 50 | 86 | 39 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 76# | 70 | 349.5 | 154 | 159* |
55# | 55 | 282 | 171 | ||||
92 | 54 | 425 | 153 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination