| Study Attribute | Value |
|---|---|
| CEBS Accession Number: | 002-01391-0004-0000-0 |
| Chemical Name: | 3,3'-Dimethylbenzidine dihydrochloride |
| CASRN: | 612-82-8 |
| NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
| NTP Study ID: | 979364_CA |
| Study Result: | Positive |
| Activation | Trial | Trial Call |
|---|---|---|
| No Activation | 1 | Negative |
| Induced Rat Liver S9 | 1_S9 | Negative |
| No Activation | 2 | Positive |
| Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
| Vehicle Control: | Negative (Not Specified) | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
| Dimethyl Sulfoxide | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | |
| Test Chemical: | 3,3'-Dimethylbenzidine dihydrochloride | 5 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
16 | 100 | 4 | 0.040 | 4.0 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
50 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
160 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| Positive Control: | Mitomycin-C | 0.25 | 100 | 30 | 0.300 | 24.0 | 18 | 0.180 | 16.0 | 10 | 0.100 | 10.0 | 2 | 0.020 | 2.0 |
| Trend: | 0.007 | 0.325 | -0.498 | ||||||||||||
| Probability: | 0.497 | 0.373 | 0.691 | ||||||||||||
| Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
| Vehicle Control: | Negative (Not Specified) | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
| Dimethyl Sulfoxide | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | |
| Test Chemical: | 3,3'-Dimethylbenzidine dihydrochloride | 5 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
16 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
50 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
160 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
500 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
| Positive Control: | Cyclophosphamide | 50 | 100 | 104 | 1.040 | 60.0 | 51 | 0.510 | 40.0 | 37 | 0.370 | 30.0 | 16 | 0.160 | 15.0 |
| Trend: | 1.006 | 0.000 | 1.006 | ||||||||||||
| Probability: | 0.157 | 0.000 | 0.157 | ||||||||||||
| Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
| Vehicle Control: | Negative (Not Specified) | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
| Dimethyl Sulfoxide | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | |
| Test Chemical: | 3,3'-Dimethylbenzidine dihydrochloride | 5 | 100 | 5 | 0.050 | 5.0 | 0 | 0.000 | 0.0 | 4 | 0.040 | 4.0 | 1 | 0.010 | 1.0 |
10 | 100 | 7 | 0.070 | 6.0 | 4 | 0.040 | 4.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | ||
20 | 100 | 14 | 0.140 | 12.0 | 6 | 0.060 | 6.0 | 8 | 0.080 | 7.0 | 0 | 0.000 | 0.0 | ||
40 | 100 | 22 | 0.220 | 17.0 | 9 | 0.090 | 8.0 | 10 | 0.100 | 9.0 | 3 | 0.030 | 2.0 | ||
60 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
80 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
100 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 0.0 | ||
| Positive Control: | Mitomycin-C | 0.5 | 100 | 37 | 0.370 | 31.0 | 15 | 0.150 | 15.0 | 15 | 0.150 | 14.0 | 7 | 0.070 | 7.0 |
| Trend: | -0.081 | 0.417 | -0.646 | ||||||||||||
| Probability: | 0.532 | 0.338 | 0.741 | ||||||||||||