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Study Information
Study Attribute Value
CEBS Accession Number: 002-01391-0004-0000-0
Chemical Name: 3,3'-Dimethylbenzidine dihydrochloride
CASRN: 612-82-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 979364_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 04/18/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3,3'-Dimethylbenzidine dihydrochloride 
5         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
16         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
50         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
160         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 30 0.300 24.0 18 0.180 16.0 10 0.100 10.0 2 0.020 2.0
Trend: 0.007 0.325 -0.498  
Probability: 0.497 0.373 0.691  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/18/1982    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3,3'-Dimethylbenzidine dihydrochloride 
5         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
16         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
50         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
160         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
500         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 104 1.040 60.0 51 0.510 40.0 37 0.370 30.0 16 0.160 15.0
Trend: 1.006 0.000 1.006  
Probability: 0.157 0.000 0.157  


Trial #:2   Activation: No Activation    Date: 09/20/1982    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3,3'-Dimethylbenzidine dihydrochloride 
5         
 100 5 0.050 5.0 0 0.000 0.0 4 0.040 4.0 1 0.010 1.0 
10         
 100 7 0.070 6.0 4 0.040 4.0 3 0.030 3.0 0 0.000 0.0 
20         
 100 14 0.140 12.0 6 0.060 6.0 8 0.080 7.0 0 0.000 0.0 
40         
 100 22 0.220 17.0 9 0.090 8.0 10 0.100 9.0 3 0.030 2.0 
60         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
80         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
100         
 100 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 1 0.010 0.0
Positive Control: Mitomycin-C 
 0.5       
 100 37 0.370 31.0 15 0.150 15.0 15 0.150 14.0 7 0.070 7.0
Trend: -0.081 0.417 -0.646  
Probability: 0.532 0.338 0.741