Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01391-0013-0000-0 |
Chemical Name: | 3,3'-Dimethylbenzidine |
CASRN: | 119-93-7 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 576643 |
Study Result: | Positive |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 93 | 104 | 103 | 37 | 37 |
85 | 105 | 94 | 37 | ||||
82 | 112 | 97 | 39 | ||||
69 | 78 | 74 | 36 | ||||
Test Chemical | 3,3'-Dimethylbenzidine | 13 | 63 | 90 | 113 | 60 | 47 |
95 | 123 | 97 | 34 | ||||
26 | 77 | 105 | 113 | 49 | |||
51 | 79 | 43 | 152 | 64 | 81* | ||
80 | 61 | 236 | 98 | ||||
64 | 47 | 8 | 286 | 201 | |||
80 | |||||||
100 | |||||||
Positive Control | Ethyl Methane Sulfonate | 500 | 32 | 23 | 957 | 992 | 1086* |
32 | 24 | 1088 | 1133 | ||||
30 | 25 | 1026 | 1134 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 63 | 104 | 56 | 29 | 25 |
58 | 106 | 48 | 27 | ||||
57 | 94 | 31 | 18 | ||||
58 | 97 | 41 | 24 | ||||
Test Chemical | 3,3'-Dimethylbenzidine | 26 | 53 | 84 | 81 | 51 | 44* |
58 | 78 | 63 | 36 | ||||
33 | 59 | 95 | 79 | 44 | 44* | ||
66 | 102 | 86 | 43 | ||||
41 | 36 | 27 | 107 | 100 | 75* | ||
39 | 54 | 58 | 50 | ||||
51 | 40 | 17 | 108 | 91 | 95* | ||
31 | 18 | 91 | 99 | ||||
64 | 18 | 3 | 60 | 112 | 94* | ||
27 | 6 | 61 | 75 | ||||
80 | |||||||
100 | |||||||
Positive Control | Ethyl Methane Sulfonate | 500 | 45 | 57 | 915 | 673 | 687* |
40 | 48 | 772 | 643 | ||||
38 | 41 | 838 | 745 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 72 | 103 | 64 | 30 | 29 |
75 | 92 | 80 | 36 | ||||
79 | 105 | 50 | 21 | ||||
Test Chemical | 3,3'-Dimethylbenzidine | 26 | 73 | 94 | 64 | 29 | 37 |
80# | 104 | 105 | 44 | ||||
33 | 82 | 100 | 83 | 34 | 41 | ||
73 | 100 | 105 | 48 | ||||
41 | 72 | 79 | 126 | 58 | 54* | ||
73 | 78 | 109 | 50 | ||||
51 | 62 | 40 | 231 | 124 | 104* | ||
74 | 65 | 188 | 84 | ||||
64 | 58 | 34 | 186 | 108 | 140* | ||
48 | 18 | 245 | 172 | ||||
80 | 28 | 4 | 162 | 195 | 228* | ||
25 | 3 | 195 | 262 | ||||
100 | |||||||
Positive Control | Ethyl Methane Sulfonate | 500 | 50 | 44 | 821 | 544 | 537* |
44 | 48 | 736 | 564 | ||||
49 | 54 | 742 | 503 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 94 | 104 | 138 | 49 | 44 |
82 | 89 | 140 | 57 | ||||
104 | 112 | 114 | 37 | ||||
87 | 95 | 89 | 34 | ||||
Test Chemical | 3,3'-Dimethylbenzidine | 13 | 66 | 77 | 92 | 47 | 47 |
72 | 90 | 80 | 37 | ||||
67 | 81 | 112 | 56 | ||||
26 | 89 | 84 | 141 | 53 | 50 | ||
90 | 79 | 118 | 44 | ||||
91 | 96 | 148 | 54 | ||||
51 | 88 | 68 | 147 | 56 | 62 | ||
106 | 89 | 237 | 74 | ||||
80 | 72 | 134 | 56 | ||||
102 | 88 | 90 | 93 | 35 | 49 | ||
85 | 79 | 131 | 52 | ||||
81 | 69 | 145 | 60 | ||||
205 | 63 | 32 | 203 | 108 | 106* | ||
66 | 33 | 173 | 87 | ||||
66 | 35 | 240 | 122 | ||||
256 | 49 | 16 | 319 | 218 | 171* | ||
48 | 14 | 203 | 142 | ||||
45 | 13 | 206 | 152 | ||||
320 | 331* | ||||||
15 | 1 | 160 | 348 | ||||
15 | 1 | 143 | 314 | ||||
400 | |||||||
Positive Control | 3-Methylcholanthrene | 5 | 77 | 80 | 281 | 122 | 140* |
79 | 89 | 413 | 175 | ||||
81 | 87 | 297 | 122 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 65 | 127 | 85 | 44 | 41 |
55 | 101 | 76 | 46 | ||||
61 | 72 | 61 | 34 | ||||
Test Chemical | 3,3'-Dimethylbenzidine | 105 | 61 | 51 | 122 | 67 | 71* |
59 | 98 | 135 | 76 | ||||
131 | 62# | 47 | 179 | 97 | 75* | ||
52# | 70 | 82 | 52 | ||||
164 | 46# | 38 | 155 | 112 | 115* | ||
53# | 48 | 186 | 117 | ||||
205 | 39# | 28 | 108 | 92 | 87* | ||
39# | 13 | 96 | 82 | ||||
256 | 53# | 30 | 127 | 80 | 83* | ||
46# | 31 | 118 | 86 | ||||
320 | |||||||
12# | 2 | 33 | 92 | ||||
400 | |||||||
Positive Control | 3-Methylcholanthrene | 5 | 57 | 85 | 387 | 226 | 221* |
54 | 83 | 348 | 216 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination