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Study Information
Study Attribute Value
CEBS Accession Number: 002-01402-0001-0000-0
Chemical Name: 3,4-Dichloronitrobenzene
CASRN: 99-54-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 759890_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Test Failure 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 01/24/1985    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1022 432 0.423 8.64 26.0 0.000
Test Chemical 3,4-Dichloronitrobenzene 
5         
 50 1014 451 0.445 9.02 26.0 5.222 
16.7       
 50 1031 509 0.494 10.18 34.5 16.796 
50         
 50 1023 502 0.491 10.04 34.5 16.090 
167         
 0 0 0 0.000 0.00 34.5 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1032 512 0.496 10.24 26.0 17.370
Mitomycin-C 
 0.01      
 5 103 170 1.650 34.00 26.0 290.462
Trend: 2.677        
Probability: 0.004        

Trial #: 2    Activation: No Activation    Date: 02/27/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1017 527 0.518 10.54 26.0 0.000
Test Chemical 3,4-Dichloronitrobenzene 
25         
 50 1024 541 0.528 10.82 26.0 1.955 
50         
 50 1012 691 0.683 13.82 32.5 31.767 
100         
 50 1004 768 0.765 15.36 32.5 47.617 
150         
 0 0 0 0.000 0.00 32.5 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1032 612 0.593 12.24 26.0 14.441
Mitomycin-C 
 0.01      
 5 101 214 2.119 42.80 26.0 308.886
Trend: 8.132        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/24/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1036 406 0.392 8.12 26.0 0.000
Test Chemical 3,4-Dichloronitrobenzene 
16.7       
 50 1021 381 0.373 7.62 26.0 -4.779 
50         
 50 999 422 0.422 8.44 26.0 7.791 
167         
 50 1037 542 0.523 10.84 26.0 33.369 
500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 993 523 0.527 10.46 26.0 34.396
Cyclophosphamide 
2         
 5 103 168 1.631 33.60 26.0 316.204
Trend: 4.898        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 02/27/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1018 553 0.543 11.06 26.0 0.000
Test Chemical 3,4-Dichloronitrobenzene 
50         
 50 1022 529 0.518 10.58 26.0 -4.714 
100         
 50 1015 536 0.528 10.72 26.0 -2.788 
150         
 50 1013 664 0.655 13.28 32.5 20.665 
250         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1022 765 0.749 15.30 26.0 37.795
Cyclophosphamide 
2         
 5 104 189 1.817 37.80 26.0 234.542
Trend: 3.056        
Probability: 0.001