COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01404-0001-0000-2
Chemical Name: 3,4-Dihydrocoumarin
CASRN: 119-84-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 733791_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 05/29/1986    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 491 0.468 9.82 26.5 0.000
Test Chemical 3,4-Dihydrocoumarin 
5         
 50 1051 414 0.394 8.28 26.5 -15.763 
16         
 50 1048 415 0.396 8.30 26.5 -15.317 
50         
 50 1048 506 0.483 10.12 26.5 3.252 
160         
 50 1048 589 0.562 11.78 26.5 20.188 
500         
 0 0 0 0.000 0.00 26.5 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1049 574 0.547 11.48 26.5 17.016
Mitomycin-C 
 0.005     
 10 210 329 1.567 32.90 26.5 235.031
Trend: 4.187        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 07/10/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 427 0.407 8.54 26.0 0.000
Test Chemical 3,4-Dihydrocoumarin 
50         
 50 1046 523 0.500 10.46 26.0 22.717 
100         
 50 1048 528 0.504 10.56 26.0 23.653 
160         
 50 1049 563 0.537 11.26 26.0 31.724 
300         
 50 1051 780 0.742 15.60 31.0 82.148
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 569 0.542 11.38 26.0 33.001
Mitomycin-C 
 0.005     
 10 209 329 1.574 32.90 26.0 286.352
Trend: 9.827        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 05/29/1986    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 518 0.495 10.36 26.0 0.000
Test Chemical 3,4-Dihydrocoumarin 
50         
 50 1049 468 0.446 9.36 26.0 -9.911 
160         
 50 1049 526 0.501 10.52 26.0 1.254 
500         
 50 1042 520 0.499 10.40 26.0 0.771 
1600         
 50 1037 667 0.643 13.34 26.0 29.882
Positive Control Cyclophosphamide 
 0.1       
 50 1046 629 0.601 12.58 26.0 21.429
Cyclophosphamide 
 0.6       
 10 209 274 1.311 27.40 26.0 164.732
Trend: 4.949        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 07/10/1986    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 514 0.491 10.28 26.0 0.000
Test Chemical 3,4-Dihydrocoumarin 
500         
 50 1048 536 0.511 10.72 26.0 4.081 
1000         
 50 1049 544 0.519 10.88 26.0 5.534 
1600         
 50 1048 593 0.566 11.86 26.0 15.149 
2000         
 36 754 551 0.731 15.31 31.0 48.713
Positive Control Cyclophosphamide 
 0.15      
 50 1051 731 0.696 14.62 26.0 41.541
Cyclophosphamide 
 0.6       
 10 210 238 1.133 23.80 26.0 130.636
Trend: 5.703        
Probability: 0.000