Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01425-0006-0000-0
Chemical Name:	3-Aminotriazole
CASRN:	61-82-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	074450
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	37r	65	135	121	66
			59	98	128	73
			44r	74	132	101
			57	102	101	59
Test Chemical	3-Aminotriazole	312.5	56	84	116	69	75
			70	87	168	80	75
		625	66#	94	117	59	62
			72	87	140	65	62
		1250	58	97	139	80	73
			69	84	135	65	73
		2500	61	95	150	82	77
			51#	76	111	73	77
		5000	64	84	153	79	84
			54	75	144	89	84
Positive Control	Ethyl Methane Sulfonate	250	53#	55	672	424	461*
Positive Control			40	52	594	497	461*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	79	89	50	21	24
			98	106	69	24
			85	97	66	26
			104	108	77	25
Test Chemical	3-Aminotriazole	1000	72	95	55	26	18
			57	92	18	11
			71	91	38	18
		2000	58	93	58	34	21
			76	121	21	9
			42	51	24	19
		3000	65	98	58	30	28
			60	109	61	34
			58	89	33	19
		4000	65	104	23	12	21
			60	79	45	25
			66	73	52	26
		5000	71	105	53	25	25
			69	120	42	20
			73	105	63	29
Positive Control	Ethyl Methane Sulfonate	250	85	75	395	156	141*
Positive Control			60	54	227	126	141*

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	96	103	145	51	50
			75	104	144	64
			88	88	81	31
			76	106	126	56
Test Chemical	3-Aminotriazole	312.5	66	95	137	70	62
			71	97	118	55	62
		625	92	98	151	55	45
			94	102	98	35	45
		1250	93	99	95	34	47
			92	94	163	59	47
		2500	80	95	94	39	37
			77	104	82	36	37
		5000	85	89	116	45	37
			83	79	73	29	37
Positive Control	3-Methylcholanthrene	2.5	66	73	235	119	131*
Positive Control			58	102	249	143	131*

Induced S9 Trial: 1 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	69	90	207	100	78
			65	111	116	59
			75	89	204	91
			83	110	152	61
Test Chemical	3-Aminotriazole	312.5	71	88	183	86	71
			77	103	130	56	71
		625	68	101	218	108	84
			85	134	152	60	84
		1250	70	85	164	78	68
			79	115	135	57	68
		2500	81	115	183	75	91
			68	94	217	106	91
		5000	100	124	246	82	108
			57	66	229	135	108
Positive Control	3-Methylcholanthrene	2.5	82	110	157	64	82
Positive Control			85	91	253	100	82

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	72	100	121	56	58
			80	110	123	51
			80	79	158	66
			94	111	167	59
Test Chemical	3-Aminotriazole	312.5	79	100	131	56	55
			93	129	149	54	55
		625	96	115	158	55	53
			92	104	140	51	53
		1250	78	104	110	47	51
			74	104	123	55	51
		2500	73	98	110	50	45
			83	125	100	40	45
		5000	88	105	130	49	53
			89	115	152	57	53
Positive Control	3-Methylcholanthrene	2.5	34	10	564	556	601*
Positive Control			30	9	581	646	601*

Induced S9 Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	103	75	37	39
			69	105	93	45
			71	101	92	43
			77	92	75	32
Test Chemical	3-Aminotriazole	312.5	87	86	173	66	69*
			71	84	155	72	69*
		625	60	70	97	54	61
			72	101	145	68	61
		1250	81	97	155	64	63*
			74	109	136	62	63*
		2500	64	107	131	68	70*
			63	101	136	72	70*
		5000	75	117	129	57	68*
			67	92	158	78	68*
Positive Control	3-Methylcholanthrene	2.5	38	11	899	796	767*
Positive Control			33	10	732	739	767*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination