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Study Information
Study Attribute Value
CEBS Accession Number: 002-01425-0006-0000-0
Chemical Name: 3-Aminotriazole
CASRN: 61-82-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 074450
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 37r 65 135 121 66
    59 98 128 73
    44r 74 132 101
    57 102 101 59
Test Chemical 3-Aminotriazole 
312.5       
 56 84 116 69 75
    70 87 168 80
  
625         
 66# 94 117 59 62
    72 87 140 65
  
1250         
 58 97 139 80 73
    69 84 135 65
  
2500         
 61 95 150 82 77
    51# 76 111 73
  
5000         
 64 84 153 79 84
    54 75 144 89
Positive Control Ethyl Methane Sulfonate 
250         
 53# 55 672 424 461*
    40 52 594 497

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 79 89 50 21 24
    98 106 69 24
    85 97 66 26
    104 108 77 25
Test Chemical 3-Aminotriazole 
1000         
 72 95 55 26 18
    57 92 18 11
    71 91 38 18
  
2000         
 58 93 58 34 21
    76 121 21 9
    42 51 24 19
  
3000         
 65 98 58 30 28
    60 109 61 34
    58 89 33 19
  
4000         
 65 104 23 12 21
    60 79 45 25
    66 73 52 26
  
5000         
 71 105 53 25 25
    69 120 42 20
    73 105 63 29
Positive Control Ethyl Methane Sulfonate 
250         
 85 75 395 156 141*
    60 54 227 126

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 96 103 145 51 50
    75 104 144 64
    88 88 81 31
    76 106 126 56
Test Chemical 3-Aminotriazole 
312.5       
 66 95 137 70 62
    71 97 118 55
  
625         
 92 98 151 55 45
    94 102 98 35
  
1250         
 93 99 95 34 47
    92 94 163 59
  
2500         
 80 95 94 39 37
    77 104 82 36
  
5000         
 85 89 116 45 37
    83 79 73 29
Positive Control 3-Methylcholanthrene 
2.5       
 66 73 235 119 131*
    58 102 249 143

Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 69 90 207 100 78
    65 111 116 59
    75 89 204 91
    83 110 152 61
Test Chemical 3-Aminotriazole 
312.5       
 71 88 183 86 71
    77 103 130 56
  
625         
 68 101 218 108 84
    85 134 152 60
  
1250         
 70 85 164 78 68
    79 115 135 57
  
2500         
 81 115 183 75 91
    68 94 217 106
  
5000         
 100 124 246 82 108
    57 66 229 135
Positive Control 3-Methylcholanthrene 
2.5       
 82 110 157 64 82
    85 91 253 100

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 72 100 121 56 58
    80 110 123 51
    80 79 158 66
    94 111 167 59
Test Chemical 3-Aminotriazole 
312.5       
 79 100 131 56 55
    93 129 149 54
  
625         
 96 115 158 55 53
    92 104 140 51
  
1250         
 78 104 110 47 51
    74 104 123 55
  
2500         
 73 98 110 50 45
    83 125 100 40
  
5000         
 88 105 130 49 53
    89 115 152 57
Positive Control 3-Methylcholanthrene 
2.5       
 34 10 564 556 601*
    30 9 581 646

Induced S9 Trial: 3 Experiment Call:
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 68 103 75 37 39
    69 105 93 45
    71 101 92 43
    77 92 75 32
Test Chemical 3-Aminotriazole 
312.5       
 87 86 173 66 69*
    71 84 155 72
  
625         
 60 70 97 54 61
    72 101 145 68
  
1250         
 81 97 155 64 63*
    74 109 136 62
  
2500         
 64 107 131 68 70*
    63 101 136 72
  
5000         
 75 117 129 57 68*
    67 92 158 78
Positive Control 3-Methylcholanthrene 
2.5       
 38 11 899 796 767*
    33 10 732 739

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination