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Study Information
Study Attribute Value
CEBS Accession Number: 002-01425-0007-0000-1
Chemical Name: 3-Aminotriazole
CASRN: 61-82-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G13828
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 94 114 74 26 26
    94 86 73 26
Test Chemical 3-Aminotriazole 
1966         
 112 137 106 31
  
2458         
 94 103 130 46 43
    108 113 129 40
  
3072         
 96 101 83 29
  
3840         
 122r 128 119 33
    84 85 113 45
  
4800         
 91 92 87 32
Positive Control Ethyl Methane Sulfonate 
500         
 46 36 786 565 538*
    52 38 799 511

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 77 99 76 33 28
    75 101 50 22
Test Chemical 3-Aminotriazole 
1573         
 86 148 79 31 39
    57 94 80 47
  
1966         
 74 121 99 45 43
    82 139 103 42
  
2458         
 84 135 128 51 49*
    77 140 108 47
  
3072         
 71 78 110 52 52*
    66 80 103 52
  
3840         
 87 91 131 50 63*
    72 72 164 76
  
6000         
 81 77 74 31
Positive Control Ethyl Methane Sulfonate 
500         
 39 25 950 805 823*
    34 25 862 841

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 83 95 98 39 27
    85 112 71 28
    98 95 60 21
    85 98 53 21
Test Chemical 3-Aminotriazole 
1258         
 105 116 102 32 35
    105 132 121 39
  
1573         
 100 132 86 29 33
    97 111 94 32
    113 143 124 37
  
1966         
 75 86 88 39 33
    112 137 121 36
    106 141 75 24
  
2458         
 107 124 109 34 32
    99 124 105 35
    106 125 87 27
  
3072         
 120 138 95 26 30
    115 141 79 23
    101 85 120 40
  
3840         
 105 80 103 33 34
    102 110 107 35
    100 125 108 36
  
4800         
 108 122 82 25 31
    109 110 129 39
    98 103 82 28
  
6000         
 95 106 91 32 36
    93 86 111 40
Positive Control Ethyl Methane Sulfonate 
500         
 58 54 805 467 476*
    58 48 934 532
    73 65 940 428

Nonactivation Trial: 4 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 99 69 123 41 48
    99 103 187 63
    114 118 125 37
    106 110 160 50
Test Chemical 3-Aminotriazole 
1560         
 124r 111 126 34
    128r 104 198 52
    120 93 188 52
  
1894         
 120 117 159 44 44
    114 107 148 43
    110 109 149 45
  
2300         
 201r 169 183 30 37
    100 105 114 38
    115 102 124 36
  
2792         
 118 96 119 34 33
    114# 101 112.5 33
  
3391         
 98 87 145 49 49
    97 82 149 51
    111 105 158 47
  
4117         
 106 94 148 47 43
    112 101 137 41
    115 110 144 42
  
5000         
 103 93 147 48 53
    97 71 170 58
Positive Control Ethyl Methane Sulfonate 
500         
 59 35 1432 809 782*
    56 32 1376 826
    64 33 1358 709

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 81 102 149 61 61
    81 79 144 59
    88 111 143 54
    86 108 174 67
Test Chemical 3-Aminotriazole 
1048         
 68 79 139 68
  
1311         
 83 92 184 74 65
    90# 105 150 56
  
1638         
 87# 84 186 71 62
    87 85 162 62
    89 88 137 51
  
2048         
 90 93 102 38 47
    74# 70 112.5 51
    102# 111 160 52
  
2560         
 85 85 148 58 65
    69 65 144 70
    92# 85 184.5 67
  
3200         
 91 82 131 48 51
    80 68 124 52
    81 75 130 53
  
4000         
 74 56 113 51 57
    81 60 168 69
    80 66 124 51
  
5000         
 72# 47 136.5 63 59
    73 42 115 53
    84# 51 153 61
Positive Control 3-Methylcholanthrene 
7         
 81 53 489 202 224*
    83 52 540 218
    62 43 467 252

Non-Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 103 97 188 61 60
    101 95 196 65
    105 99 163 52
    116 109 222 64
Test Chemical 3-Aminotriazole 
1048         
 103 100 137 44 48
    125r 112 149 40
    110 95 168 51
  
1311         
 105 99 207 66 57
    109 94 207 63
    108 94 132 41
  
1638         
 104 90 174 56 60
    104 90 179 58
    101 87 201 67
  
2048         
 106 91 158 50 49
    110 95 172 52
    100 91 139 47
  
2560         
 102 88 162 53 52
    100 76 137 46
    105 86 180 57
  
3200         
 116 95 128 37 42
    112 89 132 39
    101 77 146 48
  
4000         
 117 95 138 39 45
    105 85 169 53
    102 79 133 43
  
5000         
 104 68 195 63 48
    108 74 137 42
    116 79 139 40
Positive Control 3-Methylcholanthrene 
7         
 75 49 620 277 279*
    70 43 532 255
    78 47 720 306

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 106 113 53 56
    65 94 115 59
Test Chemical 3-Aminotriazole 
1966         
 76 118 71 31 40
    71 119 103 48
  
2458         
 82 131 121 49 44
    75 123 87 39
  
3072         
 75 123 69 31 31
    81 129 77 32
  
3840         
 80 117 120 50 46
    74 103 94 42
  
4800         
 69 98 117 57 49
    75 108 93 41
  
6000         
 78 116 94 40 41
    79 132 100 42
Positive Control 3-Methylcholanthrene 
5         
 44 29 379 290 270*
    46 31 345 250

Induced S9 Trial: 2 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 82 111 316 128 133
    72# 89 298 137
Test Chemical 3-Aminotriazole 
1573         
 94 115 108 38 44
    91 113 137 50
  
1966         
 103 126 105 34 39
    99 121 128 43
  
2458         
 86 100 106 41 42
    94 109 122 43
  
3072         
 103 119 135 44 44
    92 106 121 44
  
3840         
 96 114 122 43 47
    90 107 137 51
  
4800         
 92 101 134 48 58
    78# 86 158 68
  
6000         
 82 84 124 51 46
    96# 105 119 42
Positive Control 3-Methylcholanthrene 
5         
 37 19 525 469 469*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 88 99 132 50 55
    92 104 172 63
    82 97 127 52
Test Chemical 3-Aminotriazole 
839         
 111 126 140 42 46
    109 113 161 49
  
1048         
 103 127 112 36 38
    107 110 125 39
  
1311         
 104 122 127 41 38
    98 111 104 35
  
1638         
 103 118 154 50 44
    103 104 118 38
  
2048         
 124r 148 165 44
    102 103 161 53
  
2560         
 96 110 116 40 45
    90 94 137 51
  
3200         
 157r 164 187 40
    99 105 129 43
  
4000         
 120 124 137 38
    160r 159 185 39
  
5000         
 114 123 108 32
Positive Control 3-Methylcholanthrene 
5         
 58 29 610 350 334*
    57 26 559 329
    68 35 665 324

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination