Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01425-0008-0000-2
Chemical Name:	3-Aminotriazole
CASRN:	61-82-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G60217
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	102	155	76	63
Vehicle Control			71	54	106	50	63
Test Chemical	3-Aminotriazole	313	71	107	108	51	54
			73	87	124	57	54
		625	83	108	158	63	68
			81	119	177	73	68
		1250	73	89	119	54	52
			64	101	96	50	52
		2500	71	94	105	49	61
			76#	107	165	72	61
		5000	81	99	163	67	67
			73	90	146	67	67
Positive Control	Ethyl Methane Sulfonate	500	31	32	888	955	926*
Positive Control			34	33	906	897	926*
Negative Control	Negative (Not Specified)	0	92	123	155	56	48*
Negative Control			92	121	110	40	48*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	112	107	78	23	29
Vehicle Control			78#	77	79.5	34	29
Test Chemical	3-Aminotriazole	1250	86	89	66	26	26
			92#	96	70.5	25	26
		2500	104	92	60	19	26
			83	89	81	33	26
		5000	105	79	118	38	37
			98	87	106	36	37
		7500	107	84	128	40	37
			115	86	119	35	37
		9999.9	95	59	130	46	40
			99#	72	101	34	40
Positive Control	Ethyl Methane Sulfonate	500	49	21	827	566	611*
Positive Control			40	17	790	656	611*
Negative Control	Negative (Not Specified)	0	114	110	74	22	20*
Negative Control			111	107	63	19	20*

Non-Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	55	88	164	100	95
Vehicle Control			58	107	156	89	95
Test Chemical	3-Aminotriazole	313	51	143	67	44	50
			69	120	115	56	50
		625	70	163	94	45	57
			57	133	120	70	57
		1250	52	115	99	64	57
			55	118	85	51	57
		2500	54	139	97	60	57
			44#	98	72	55	57
		5000	61#	126	78	43	41
			62	148	73	39	41
Positive Control	3-Methylcholanthrene	7	52#	23	675	429	482*
Positive Control			48	14	767	536	482*
Negative Control	Negative (Not Specified)	0	63	102	214	113	103*
Negative Control			63#	104	175	93	103*

Non-Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	104	119	191	61	57
			98	89	200	68
			97	91	155	53
			96	101	132	46
Test Chemical	3-Aminotriazole	156	99	123	126	43	46
			93	96	139	50	46
		313	81	97	111	46	45
			92	102	119	43	45
		625	75	90	106	47	42
			87	88	97	37	42
		1250	91	74	156	57	59
			71#	89	129	61	59
		2500	75	71	109	48	62
			84	69	190	75	62
		5000	93	87	137	49	49
			87	86	127	49	49
Positive Control	3-Methylcholanthrene	7	68	36	609	297	283*
Positive Control			75	27	606	269	283*

Non-Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	63	82	65	35	30
			87	116	60	23
			84	119	66	26
			92	83	94	34
Test Chemical	3-Aminotriazole	156	104	112	104	33	34
			88	118	91	34	34
		313	113	133	46	14	21
			96	109	82	29	21
		625	86	101	61	24	23
			119	117	77	22	23
		1250	103	135	38	12
			139r	110	67	16
		2500	108	121	51	16	19
			82	106	56	23	19
		5000	104	136	54	17	21
			107	130	76	24	21
Positive Control	3-Methylcholanthrene	7	70	48	415	197	168*
			80	51	360	150
			86	54	401	156

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	74#	43	144	65	52
Vehicle Control			64	141	76	40	52
Test Chemical	3-Aminotriazole	1250	76	137	68	30	34
			76	128	87	38	34
		2500	78	110	78	33	36
			95	139	108	38	36
		5000	72	106	106	49	44
			66#	99	76.5	39	44
		7500	98	144	117	40	48
			61	99	103	56	48
		9999.9	71	112	98	46	47
			73	20	105	48	47
Positive Control	3-Methylcholanthrene	5	47	25	543	382	340*
Positive Control			55	47	491	297	340*
Negative Control	Negative (Not Specified)	0	66	80	153	77	63*
Negative Control			86	136	125	48	63*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	102	107	203	66	80
			97	110	216	75
			84	97	213	85
			75	86	216	96
Test Chemical	3-Aminotriazole	156	103	142	172	56	62
			98	114	199	68	62
		313	85	127	146	57	52
			93	165	130	47	52
		625	96	125	174	61	53
			111	148	149	45	53
		1250	97	172	121	42	43
			107	168	139	43	43
		2500	95	165	150	53	56
			90	142	157	58	56
		5000	90	159	157	58	59
			89	113	157	59	59
Positive Control	3-Methylcholanthrene	5	35	8	568	546	552*
Positive Control			33	10	553	559	552*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination