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Study Information
Study Attribute Value
CEBS Accession Number: 002-01432-0001-0000-3
Chemical Name: 3-Chloro-2-methylpropene
CASRN: 563-47-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 087115_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Weakly Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 05/24/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1049 406 0.387 8.12 26.0 -11.992
Dimethyl Sulfoxide 
0         
 50 1046 460 0.440 9.20 26.0 0.000
Test Chemical 3-Chloro-2-methylpropene 
5         
 50 1050 548 0.522 10.96 26.0 18.677 
16         
 50 1049 598 0.570 11.96 26.0 29.628 
50         
 50 1047 610 0.583 12.20 26.0 32.482 
160         
 50 1051 1091 1.038 21.82 26.0 136.046 
500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1051 1317 1.253 26.34 26.0 184.942
Mitomycin-C 
 0.01      
 10 209 619 2.962 61.90 26.0 573.470
Trend: 16.239        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 06/19/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 450 0.430 9.00 26.5 0.000
Negative (Not Specified) 
0         
 50 1049 424 0.404 8.48 26.5 -5.957
Test Chemical 3-Chloro-2-methylpropene 
5         
 50 1051 505 0.480 10.10 26.5 11.795 
10         
 50 1048 482 0.460 9.64 26.5 7.009 
25         
 50 1050 528 0.503 10.56 26.5 16.998 
50         
 50 1050 650 0.619 13.00 26.5 44.032 
100         
 50 1046 894 0.855 17.88 26.5 98.857 
200         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.005     
 50 1049 2115 2.016 42.30 26.5 369.104
Mitomycin-C 
 0.01      
 10 210 648 3.086 64.80 26.5 617.943
Trend: 13.240        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 05/24/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1037 395 0.381 7.90 26.0 -8.124
Dimethyl Sulfoxide 
0         
 50 1042 432 0.415 8.64 26.0 0.000
Test Chemical 3-Chloro-2-methylpropene 
5         
 50 1044 465 0.445 9.30 26.0 7.433 
16         
 50 1048 501 0.478 10.02 26.0 15.308 
50         
 50 1046 563 0.538 11.26 26.0 29.826 
160         
 50 1035 778 0.752 15.56 26.0 81.311 
500         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1040 701 0.674 14.02 26.0 62.581
Cyclophosphamide 
2         
 10 209 339 1.622 33.90 26.0 291.235
Trend: 10.779        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 06/19/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1042 461 0.442 9.22 26.0 0.000
Negative (Not Specified) 
0         
 50 1046 483 0.462 9.66 26.0 4.372
Test Chemical 3-Chloro-2-methylpropene 
10         
 50 1048 462 0.441 9.24 26.0 -0.357 
25         
 18 374 198 0.529 11.00 26.0 19.663 
50         
 50 1045 535 0.512 10.70 26.0 15.719 
100         
 50 1045 589 0.564 11.78 26.0 27.399 
200         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1049 712 0.679 14.24 26.0 53.416
Cyclophosphamide 
2         
 10 209 394 1.885 39.40 26.0 326.105
Trend: 4.585        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 07/10/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 418 0.398 8.36 27.0 0.000
Negative (Not Specified) 
0         
 50 1052 417 0.396 8.34 27.0 -0.429
Test Chemical 3-Chloro-2-methylpropene 
100         
 50 1044 648 0.621 12.96 26.0 55.915 
125         
 50 1027 649 0.632 12.98 26.0 58.740 
150         
 50 1044 958 0.918 19.16 31.0 130.504 
175         
 0 0 0 0.000 0.00 0.0 N/A 
200         
 50 1045 840 0.804 16.80 31.0 101.918
Positive Control Cyclophosphamide 
 0.3       
 50 1052 765 0.727 15.30 27.0 82.666
Cyclophosphamide 
2         
 10 210 493 2.348 49.30 27.0 489.713
Trend: 13.163        
Probability: 0.000