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Study Information
Study Attribute Value
CEBS Accession Number: 002-01432-0002-0000-4
Chemical Name: 3-Chloro-2-methylpropene
CASRN: 563-47-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 087115_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive* 
No Activation  2  Weakly Positive* 
Induced Rat Liver S9  2_S9  Negative 
No Activation  3  Positive 

Trial #:1   Activation: No Activation    Date: 06/21/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3-Chloro-2-methylpropene 
10         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
25         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
50         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
100         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
200         
 100 9 0.090 8.0 0 0.000 0.0 9 0.090 8.0 0 0.000 0.0 
300         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 28 0.280 22.0 13 0.130 10.0 15 0.150 15.0 0 0.000 0.0
Mitomycin-C 
1         
 50 43 0.860 50.0 26 0.520 34.0 17 0.340 28.0 0 0.000 0.0
Trend: 2.594 -1.516 3.604  
Probability: 0.005 0.935 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 06/21/1984    Harvest Time: 12.8 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3-Chloro-2-methylpropene 
25         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
50         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
100         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
200         
 100 70 0.700 70.0 0 0.000 0.0 0 0.000 0.0 70 0.700 70.0 
300         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 26 0.260 25.0 13 0.130 12.0 13 0.130 13.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 54 1.080 32.0 21 0.420 32.0 13 0.260 26.0 20 0.400 2.0
Trend: 12.608 0.708 -0.030  
Probability: 0.000 0.240 0.512  


Trial #:2   Activation: No Activation    Date: 05/23/1984    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3-Chloro-2-methylpropene 
5         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
16         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
50         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
160         
 100 11 0.110 11.0 5 0.050 5.0 5 0.050 5.0 1 0.010 1.0 
500         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 53 0.530 40.0 38 0.380 31.0 15 0.150 13.0 0 0.000 0.0
Mitomycin-C 
1         
 100 72 0.720 48.0 57 0.570 43.0 15 0.150 12.0 0 0.000 0.0
Trend: 3.978 3.181 2.155  
Probability: 0.000 0.001 0.016  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 05/22/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3-Chloro-2-methylpropene 
5         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
16         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
50         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
160         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
500         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 55 0.550 40.0 31 0.310 25.0 24 0.240 21.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 72 1.440 68.0 34 0.680 48.0 28 0.560 36.0 10 0.200 1.0
Trend: 2.861 1.501 2.459  
Probability: 0.002 0.067 0.007  


Trial #:3   Activation: No Activation    Date: 01/31/1985    Harvest Time: 13.3 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 3-Chloro-2-methylpropene 
120         
 100 7 0.070 7.0 2 0.020 2.0 5 0.050 5.0 0 0.000 0.0 
160         
 100 8 0.080 8.0 6 0.060 6.0 2 0.020 2.0 0 0.000 0.0 
200         
 100 13 0.130 12.0 4 0.040 4.0 9 0.090 9.0 0 0.000 0.0 
240         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 26 0.260 23.0 13 0.130 13.0 12 0.120 11.0 1 0.010 1.0
Mitomycin-C 
 0.25      
 50 21 0.420 28.0 12 0.240 16.0 9 0.180 18.0 0 0.000 0.0
Trend: 3.259 2.154 2.633  
Probability: 0.001 0.016 0.004