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Study Information
Study Attribute Value
CEBS Accession Number: 002-01432-0013-0000-6
Chemical Name: 3-Chloro-2-methylpropene
CASRN: 563-47-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 563589
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96 119 136 47 60
    96# 98 136.5 47
    78# 89 208.5 89
    98 95 166 57
Test Chemical 3-Chloro-2-methylpropene 
3.13      
 100# 100 190.5 64 58
    91# 92 142.5 52
  
6.25      
 94 110 115 41 47
    99 106 160 54
  
12.5       
 101# 85 211.5 70 66
    107 82 161 50
    94# 79 220.5 78
  
25         
 79# 39 247.5 104 101*
    79 45 212 89
    85# 38 278 109
  
50         
 63# 7 522 278 323*
    62 6 595 321
    59 5 650 369
  
100         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 52# 33 972 629 568*
    61# 36 949.5 519
    60# 32 1006 557

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96 119 44 15 17
    96# 98 33 11
    78# 89 63 27
    98 95 46 16
Test Chemical 3-Chloro-2-methylpropene 
3.13      
 100# 100 48 16 14
    91# 92 33 12
  
6.25      
 94 110 32 11 12
    99 106 40 13
  
12.5       
 101# 85 64.5 21 19
    107 82 50 16
    94# 79 57 20
  
25         
 79 45 60 25 28*
    85# 38 77 30
    79 39 67 28
  
50         
 63# 7 67.5 36 51*
    62 6 92 50
    59 5 120 68
  
100         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 52# 33 223.5 145 127*
    61# 36 238.5 130
    60# 32 194 107

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 88 89 134 51 46
    67# 73 136 68
    95 123 92 32
    77 115 80 35
Test Chemical 3-Chloro-2-methylpropene 
20         
 16r 15 0 0 14
    95 159 54 19
    18 26 5 10
  
30         
 97 121 111 38 37
    130r 104 163 42
    102 105 113 37
  
40         
 78 67 159 68 63
    79 74 168 71
    110 107 160 49
  
50         
 90 43 194 72 77*
    98# 60 218 75
    95 55 239 84
  
60         
 123r 81 273 74 75*
    103 66 241 78
    117 78 251 72
  
80         
 68 16 369 182 258*
    61 9 467 257
    54 10 545 335
  
100         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 63 46 789 417 410*
    68 44 871 428
    87 56 1000 384

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination