Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01432-0013-0000-6
Chemical Name:	3-Chloro-2-methylpropene
CASRN:	563-47-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	563589
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96	119	136	47	60
			96#	98	136.5	47
			78#	89	208.5	89
			98	95	166	57
Test Chemical	3-Chloro-2-methylpropene	3.13	100#	100	190.5	64	58
			91#	92	142.5	52	58
		6.25	94	110	115	41	47
			99	106	160	54	47
		12.5	101#	85	211.5	70	66
			107	82	161	50
			94#	79	220.5	78
		25	79#	39	247.5	104	101*
			79	45	212	89
			85#	38	278	109
		50	63#	7	522	278	323*
			62	6	595	321
			59	5	650	369
		100


Positive Control	Ethyl Methane Sulfonate	250	52#	33	972	629	568*
			61#	36	949.5	519
			60#	32	1006	557

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96	119	44	15	17
			96#	98	33	11
			78#	89	63	27
			98	95	46	16
Test Chemical	3-Chloro-2-methylpropene	3.13	100#	100	48	16	14
			91#	92	33	12	14
		6.25	94	110	32	11	12
			99	106	40	13	12
		12.5	101#	85	64.5	21	19
			107	82	50	16
			94#	79	57	20
		25	79	45	60	25	28*
			85#	38	77	30
			79	39	67	28
		50	63#	7	67.5	36	51*
			62	6	92	50
			59	5	120	68
		100


Positive Control	Ethyl Methane Sulfonate	250	52#	33	223.5	145	127*
			61#	36	238.5	130
			60#	32	194	107

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	88	89	134	51	46
			67#	73	136	68
			95	123	92	32
			77	115	80	35
Test Chemical	3-Chloro-2-methylpropene	20	16r	15	0	0	14
			95	159	54	19
			18	26	5	10
		30	97	121	111	38	37
			130r	104	163	42
			102	105	113	37
		40	78	67	159	68	63
			79	74	168	71
			110	107	160	49
		50	90	43	194	72	77*
			98#	60	218	75
			95	55	239	84
		60	123r	81	273	74	75*
			103	66	241	78
			117	78	251	72
		80	68	16	369	182	258*
			61	9	467	257
			54	10	545	335
		100


Positive Control	Ethyl Methane Sulfonate	250	63	46	789	417	410*
			68	44	871	428
			87	56	1000	384

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination