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Study Information
Study Attribute Value
CEBS Accession Number: 002-01435-0007-0000-2
Chemical Name: 3-Chloro-p-toluidine
CASRN: 95-74-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 836474
Study Result: Equivocal


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 105 108 99 31 30
    111# 120 78 23
    105 83 88 28
    105 89 112 35
Test Chemical 3-Chloro-p-toluidine 
25         
 107 65 123 38 29
    107 99 78 24
    103 52 73 24
  
50         
 111 52 122 37 35
    107 74 106 33
    101 36 107 35
  
75         
 111 37 143 43 43
    108 27 140 43
    110 20 135 41
  
100         
 94 12 112 40 32
    110 24 92 28
    105 13 92 29
  
150         
 113 51 103 30 43
    60 5 88 49
    72 4 106 49
  
200         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 56# 33 1049 623 433*
    72 30 645 297
    78 47 885 381

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 85 96 137 54 58
    74 87 177 80
    126r 150 185 49
    116 117 136 39
Test Chemical 3-Chloro-p-toluidine 
50         
 104 99 121 39 33
    110 111 107 32
    91 90 78 29
  
75         
 87 71 123 47 57
    63 58 82 44
    50 37 122 81
  
100         
 59 48 107 60 49
    73 58 88 40
    83 82 116 47
  
150         
 84 38 118 47 48
    67 50 119 59
    73 40 82 37
  
200         
 91 35 183 67 63
    98 24 190 65
    90 30 157 58
Positive Control Methyl Methane Sulfonate 
5         
 69 53 955 461 362*
    98 79 741 252
    74 52 825 373

Nonactivation Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 126r 86 102 27 38
    107# 102 86 27
    81 108 118 49
    87 90 99 38
Test Chemical 3-Chloro-p-toluidine 
100         
 68 39 62 30 33
    77 57 65 28
    74 46 91 41
  
125         
 77 38 89 39 47
    68 21 130 64
    73 35 82 37
  
150         
 92 21 126 46 44
    99 37 149 50
    100 31 103 34
  
175         
 96 17 142 50 49
    78 15 106 46
    84 27 129 51
  
200         
 103 21 123 40 50
    83 15 167 67
    104 20 134 43
  
250         
 70*
    81 5 183 75
    78 7 150 64
  
300         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 110 73 522 159 178*
    101 64 583 192
    103 77 565 183

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 117 87 159 45 55
    95 101 184 65
    104 96 173 56
    115 115 185 54
Test Chemical 3-Chloro-p-toluidine 
5         
 92 41 233 85 91*
    71 41 172 81
    59 34 191 108
  
10         
 79 25 214 90 86*
    61# 28 175 95
    109 45 236 72
  
20         
 61 22 190 104 109*
    57 24 146 85
    61 24 250 137
  
40         
 92 8 193 70 77
    86 17 178 69
    83 16 231 93
  
50         
 74
    99 11 215 72
    113 6 256 76
  
60         
 126r 7 338 90
    95 8 227 80
   
  
80         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 68# 39 532 260 216*
    83 39 516 207
    55 28 301 181

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 103 105 65 21 40
    68 95 74 36
    68 96 122 60
    69 104 89 43
Test Chemical 3-Chloro-p-toluidine 
10         
 34# 29 130 126 79*
    59 57 108 61
    79 64 117 49
  
20         
 54 30 122 76 61*
    91 56 113 41
    54 43 107 66
  
30         
 79 37 145 61 65*
    61 19 150 83
    76 26 116 51
  
40         
 110 20 229 69 96*
    61 30 211 115
    53 19 164 103
  
50         
 87 17 281 108 100*
    59 9 208 118
    92 28 202 74
  
60         
 106 33 210 66 66*
    94 22 238 84
    77 14 111 48
Positive Control 3-Methylcholanthrene 
2.5       
 113 78 330 98 139*
    66 72 291 148
    65# 61 337 172

Induced S9 Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 111 72 304 91 76
    93 127 228 81
    91 136 224 83
    93 64 142 51
Test Chemical 3-Chloro-p-toluidine 
10         
 107 89 227 71 87
    82 77 225 92
    93 94 276 99
  
20         
 93 84 243 87 100
    78 67 288 124
    96 79 254 89
  
30         
 118 67 288 82 98
    94 82 223 79
    108 50 429 132
  
40         
 137r 56 413 100 129*
    105 49 477 151
    104 49 334 107
  
50         
 147r 52 321 73
    123r 45 313 85
    104 27 306 98
  
60         
    121r 21 407 112
    106 23 266 84
  
80         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 68 57 829 409 352*
    100 51 931 311
    90 52 900 335

Induced S9 Trial: 4 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 69# 98 284 137 124
    73 103 270 123
    73 100 348 159
    69 99 163 79
Test Chemical 3-Chloro-p-toluidine 
10         
 102 72 397 130 140
    103 69 486 158
    98 76 386 131
  
20         
 97 35 571 196 155
    108 37 385 119
    94 38 425 152
  
30         
 85 21 466 184 177
    71 17 373 175
    75 16 388 173
  
40         
 92 12 651 237 197*
    108 16 574 177
    74 13 394 177
  
50         
 93 18 404 144 154
    96 11 408 141
    88 12 464 175
  
60         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 74 33 1147 517 568*
    50 21 918 610
    57 27 986 577

Induced S9 Trial: 5 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 79 101 186 78 85
    71# 105 189 89
    81 93 217 89
Test Chemical 3-Chloro-p-toluidine 
10         
 68 47 199 97 114
    83 45 287 115
    84 40 327 130
  
20         
 106 30 438 138 125*
    82 24 263 107
    66 27 258 131
  
30         
 83 20 480 193 165*
    92 20 372 135
    66 16 331 168
  
40         
 77 9 400 173 155*
    74 11 275 124
    86 9 431 168
  
50         
 110 18 326 99 108
    104# 20 317 102
    92 13 342 124
  
60         
 86 15 343 133 137*
    76 7 316 138
    64 7 266 139
  
80         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 77# 51 488 212 268*
    72 54 650 302
    89 73 773 291

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination