Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01435-0007-0000-2
Chemical Name:	3-Chloro-p-toluidine
CASRN:	95-74-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	836474
Study Result:	Equivocal

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	105	108	99	31	30
			111#	120	78	23
			105	83	88	28
			105	89	112	35
Test Chemical	3-Chloro-p-toluidine	25	107	65	123	38	29
			107	99	78	24
			103	52	73	24
		50	111	52	122	37	35
			107	74	106	33
			101	36	107	35
		75	111	37	143	43	43
			108	27	140	43
			110	20	135	41
		100	94	12	112	40	32
			110	24	92	28
			105	13	92	29
		150	113	51	103	30	43
			60	5	88	49
			72	4	106	49
		200


Positive Control	Methyl Methane Sulfonate	5	56#	33	1049	623	433*
			72	30	645	297
			78	47	885	381

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	85	96	137	54	58
			74	87	177	80
			126r	150	185	49
			116	117	136	39
Test Chemical	3-Chloro-p-toluidine	50	104	99	121	39	33
			110	111	107	32
			91	90	78	29
		75	87	71	123	47	57
			63	58	82	44
			50	37	122	81
		100	59	48	107	60	49
			73	58	88	40
			83	82	116	47
		150	84	38	118	47	48
			67	50	119	59
			73	40	82	37
		200	91	35	183	67	63
			98	24	190	65
			90	30	157	58
Positive Control	Methyl Methane Sulfonate	5	69	53	955	461	362*
			98	79	741	252
			74	52	825	373

Nonactivation Trial: 3 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	126r	86	102	27	38
			107#	102	86	27
			81	108	118	49
			87	90	99	38
Test Chemical	3-Chloro-p-toluidine	100	68	39	62	30	33
			77	57	65	28
			74	46	91	41
		125	77	38	89	39	47
			68	21	130	64
			73	35	82	37
		150	92	21	126	46	44
			99	37	149	50
			100	31	103	34
		175	96	17	142	50	49
			78	15	106	46
			84	27	129	51
		200	103	21	123	40	50
			83	15	167	67
			104	20	134	43
		250					70*
			81	5	183	75
			78	7	150	64
		300


Positive Control	Methyl Methane Sulfonate	5	110	73	522	159	178*
			101	64	583	192
			103	77	565	183

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	117	87	159	45	55
			95	101	184	65
			104	96	173	56
			115	115	185	54
Test Chemical	3-Chloro-p-toluidine	5	92	41	233	85	91*
			71	41	172	81
			59	34	191	108
		10	79	25	214	90	86*
			61#	28	175	95
			109	45	236	72
		20	61	22	190	104	109*
			57	24	146	85
			61	24	250	137
		40	92	8	193	70	77
			86	17	178	69
			83	16	231	93
		50					74
			99	11	215	72
			113	6	256	76
		60	126r	7	338	90
			95	8	227	80

		80


Positive Control	3-Methylcholanthrene	2.5	68#	39	532	260	216*
			83	39	516	207
			55	28	301	181

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	103	105	65	21	40
			68	95	74	36
			68	96	122	60
			69	104	89	43
Test Chemical	3-Chloro-p-toluidine	10	34#	29	130	126	79*
			59	57	108	61
			79	64	117	49
		20	54	30	122	76	61*
			91	56	113	41
			54	43	107	66
		30	79	37	145	61	65*
			61	19	150	83
			76	26	116	51
		40	110	20	229	69	96*
			61	30	211	115
			53	19	164	103
		50	87	17	281	108	100*
			59	9	208	118
			92	28	202	74
		60	106	33	210	66	66*
			94	22	238	84
			77	14	111	48
Positive Control	3-Methylcholanthrene	2.5	113	78	330	98	139*
			66	72	291	148
			65#	61	337	172

Induced S9 Trial: 3 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	111	72	304	91	76
			93	127	228	81
			91	136	224	83
			93	64	142	51
Test Chemical	3-Chloro-p-toluidine	10	107	89	227	71	87
			82	77	225	92
			93	94	276	99
		20	93	84	243	87	100
			78	67	288	124
			96	79	254	89
		30	118	67	288	82	98
			94	82	223	79
			108	50	429	132
		40	137r	56	413	100	129*
			105	49	477	151
			104	49	334	107
		50	147r	52	321	73
			123r	45	313	85
			104	27	306	98
		60
			121r	21	407	112
			106	23	266	84
		80


Positive Control	3-Methylcholanthrene	2.5	68	57	829	409	352*
			100	51	931	311
			90	52	900	335

Induced S9 Trial: 4 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	69#	98	284	137	124
			73	103	270	123
			73	100	348	159
			69	99	163	79
Test Chemical	3-Chloro-p-toluidine	10	102	72	397	130	140
			103	69	486	158
			98	76	386	131
		20	97	35	571	196	155
			108	37	385	119
			94	38	425	152
		30	85	21	466	184	177
			71	17	373	175
			75	16	388	173
		40	92	12	651	237	197*
			108	16	574	177
			74	13	394	177
		50	93	18	404	144	154
			96	11	408	141
			88	12	464	175
		60


Positive Control	3-Methylcholanthrene	2.5	74	33	1147	517	568*
			50	21	918	610
			57	27	986	577

Induced S9 Trial: 5 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	79	101	186	78	85
			71#	105	189	89
			81	93	217	89
Test Chemical	3-Chloro-p-toluidine	10	68	47	199	97	114
			83	45	287	115
			84	40	327	130
		20	106	30	438	138	125*
			82	24	263	107
			66	27	258	131
		30	83	20	480	193	165*
			92	20	372	135
			66	16	331	168
		40	77	9	400	173	155*
			74	11	275	124
			86	9	431	168
		50	110	18	326	99	108
			104#	20	317	102
			92	13	342	124
		60	86	15	343	133	137*
			76	7	316	138
			64	7	266	139
		80


Positive Control	3-Methylcholanthrene	2.5	77#	51	488	212	268*
			72	54	650	302
			89	73	773	291

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination